The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates
Ascidians are the closest living relatives of vertebrates, and their study is important for understanding the evolutionary processes of oocyte maturation and ovulation. In this study, we first examined the ovulation of Ciona intestinalis Type A by monitoring follicle rupture in vitro, identifying a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/49062 |
| _version_ | 1811201361568595968 |
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| author | Shin Matsubara Akira Shiraishi Tomohiro Osugi Tsuyoshi Kawada Honoo Satake |
| author_facet | Shin Matsubara Akira Shiraishi Tomohiro Osugi Tsuyoshi Kawada Honoo Satake |
| author_sort | Shin Matsubara |
| collection | DOAJ |
| description | Ascidians are the closest living relatives of vertebrates, and their study is important for understanding the evolutionary processes of oocyte maturation and ovulation. In this study, we first examined the ovulation of Ciona intestinalis Type A by monitoring follicle rupture in vitro, identifying a novel mechanism of neuropeptidergic regulation of oocyte maturation and ovulation. Ciona vasopressin family peptide (CiVP) directly upregulated the phosphorylation of extracellular signal–regulated kinase (CiErk1/2) via its receptor. CiVP ultimately activated a maturation-promoting factor, leading to oocyte maturation via germinal vesicle breakdown. CiErk1/2 also induced expression of matrix metalloproteinase (CiMMP2/9/13) in the oocyte, resulting in collagen degradation in the outer follicular cell layer and liberation of fertile oocytes from the ovary. This is the first demonstration of essential pathways regulating oocyte maturation and ovulation in ascidians and will facilitate investigations of the evolutionary process of peptidergic regulation of oocyte maturation and ovulation throughout the phylum Chordata. |
| first_indexed | 2024-04-12T02:20:36Z |
| format | Article |
| id | doaj.art-e65d0aef59354dcaab66c9d53f66235d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:20:36Z |
| publishDate | 2019-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e65d0aef59354dcaab66c9d53f66235d2022-12-22T03:52:08ZengeLife Sciences Publications LtdeLife2050-084X2019-10-01810.7554/eLife.49062The regulation of oocyte maturation and ovulation in the closest sister group of vertebratesShin Matsubara0https://orcid.org/0000-0002-7794-534XAkira Shiraishi1https://orcid.org/0000-0003-3456-3074Tomohiro Osugi2https://orcid.org/0000-0001-6987-9576Tsuyoshi Kawada3Honoo Satake4https://orcid.org/0000-0003-1165-3624Bioorganic Research Institute, Suntory Foundation for Life Sciences, Kyoto, Japan; Research Fellow of Japan Society for the Promotion of Science, Tokyo, JapanBioorganic Research Institute, Suntory Foundation for Life Sciences, Kyoto, JapanBioorganic Research Institute, Suntory Foundation for Life Sciences, Kyoto, JapanBioorganic Research Institute, Suntory Foundation for Life Sciences, Kyoto, JapanBioorganic Research Institute, Suntory Foundation for Life Sciences, Kyoto, JapanAscidians are the closest living relatives of vertebrates, and their study is important for understanding the evolutionary processes of oocyte maturation and ovulation. In this study, we first examined the ovulation of Ciona intestinalis Type A by monitoring follicle rupture in vitro, identifying a novel mechanism of neuropeptidergic regulation of oocyte maturation and ovulation. Ciona vasopressin family peptide (CiVP) directly upregulated the phosphorylation of extracellular signal–regulated kinase (CiErk1/2) via its receptor. CiVP ultimately activated a maturation-promoting factor, leading to oocyte maturation via germinal vesicle breakdown. CiErk1/2 also induced expression of matrix metalloproteinase (CiMMP2/9/13) in the oocyte, resulting in collagen degradation in the outer follicular cell layer and liberation of fertile oocytes from the ovary. This is the first demonstration of essential pathways regulating oocyte maturation and ovulation in ascidians and will facilitate investigations of the evolutionary process of peptidergic regulation of oocyte maturation and ovulation throughout the phylum Chordata.https://elifesciences.org/articles/49062ovulationfolliclematurationvasopressin |
| spellingShingle | Shin Matsubara Akira Shiraishi Tomohiro Osugi Tsuyoshi Kawada Honoo Satake The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates eLife ovulation follicle maturation vasopressin |
| title | The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates |
| title_full | The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates |
| title_fullStr | The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates |
| title_full_unstemmed | The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates |
| title_short | The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates |
| title_sort | regulation of oocyte maturation and ovulation in the closest sister group of vertebrates |
| topic | ovulation follicle maturation vasopressin |
| url | https://elifesciences.org/articles/49062 |
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