Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis
Objective The purpose of this study was to perform a comparative analysis of metabolite levels in serum and milk obtained from cows fed on different concentrate to forage feed ratios. Methods Eight lactating Holstein cows were divided into two groups: a high forage ratio diet (HF; 80% Italian ryegra...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2023-08-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-22-0486.pdf |
| _version_ | 1827908299161337856 |
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| author | Hyun Sang Kim Jun Sik Eom Shin Ja Lee Youyoung Choi Seong Uk Jo Sang Suk Lee Eun Tae Kim Sung Sill Lee |
| author_facet | Hyun Sang Kim Jun Sik Eom Shin Ja Lee Youyoung Choi Seong Uk Jo Sang Suk Lee Eun Tae Kim Sung Sill Lee |
| author_sort | Hyun Sang Kim |
| collection | DOAJ |
| description | Objective The purpose of this study was to perform a comparative analysis of metabolite levels in serum and milk obtained from cows fed on different concentrate to forage feed ratios. Methods Eight lactating Holstein cows were divided into two groups: a high forage ratio diet (HF; 80% Italian ryegrass and 20% concentrate of daily intake of dry matter) group and a high concentrate diet (HC; 20% Italian ryegrass and 80% concentrate) group. Blood was collected from the jugular vein, and milk was sampled using a milking machine. Metabolite levels in serum and milk were estimated using proton nuclear magnetic resonance and subjected to qualitative and quantitative analyses performed using Chenomx 8.4. For statistical analysis, Student’s t-test and multivariate analysis were performed using Metaboanalyst 4.0. Results In the principal component analysis, a clear distinction between the two groups regarding milk metabolites while serum metabolites were shown in similar. In serum, 95 metabolites were identified, and 13 metabolites (include leucine, lactulose, glucose, betaine, etc.) showed significant differences between the two groups. In milk, 122 metabolites were identified, and 20 metabolites (include urea, carnitine, acetate, butyrate, arabinitol, etc.) showed significant differences. Conclusion Our results show that different concentrate to forage feed ratios impact the metabolite levels in the serum and milk of lactating Holstein cows. A higher number of metabolites in milk, including those associated with milk fat synthesis and the presence of Escherichia coli in the rumen, differed between the two groups compared to that in the serum. The results of this study provide a useful insight into the metabolites associated with different concentrate to forge feed ratios in cows and may aid in the search for potential biomarkers for subacute ruminal acidosis. |
| first_indexed | 2024-03-13T01:19:55Z |
| format | Article |
| id | doaj.art-e65ee1cc4c05401b8f14ed60ba65e3c4 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-03-13T01:19:55Z |
| publishDate | 2023-08-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-e65ee1cc4c05401b8f14ed60ba65e3c42023-07-05T04:53:32ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352023-08-013681199120810.5713/ab.22.048625022Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosisHyun Sang Kim0Jun Sik Eom1Shin Ja Lee2Youyoung Choi3Seong Uk Jo4Sang Suk Lee5Eun Tae Kim6Sung Sill Lee7 Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea Division of Applied Life Science (BK21), Gyeongsang National University, Jinju 52828, Korea Division of Applied Life Science (BK21), Gyeongsang National University, Jinju 52828, Korea Ruminant Nutrition and Anaerobe Laboratory, College of Bio-industry Science, Sunchon National University, Suncheon 57922, Korea National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Korea Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaObjective The purpose of this study was to perform a comparative analysis of metabolite levels in serum and milk obtained from cows fed on different concentrate to forage feed ratios. Methods Eight lactating Holstein cows were divided into two groups: a high forage ratio diet (HF; 80% Italian ryegrass and 20% concentrate of daily intake of dry matter) group and a high concentrate diet (HC; 20% Italian ryegrass and 80% concentrate) group. Blood was collected from the jugular vein, and milk was sampled using a milking machine. Metabolite levels in serum and milk were estimated using proton nuclear magnetic resonance and subjected to qualitative and quantitative analyses performed using Chenomx 8.4. For statistical analysis, Student’s t-test and multivariate analysis were performed using Metaboanalyst 4.0. Results In the principal component analysis, a clear distinction between the two groups regarding milk metabolites while serum metabolites were shown in similar. In serum, 95 metabolites were identified, and 13 metabolites (include leucine, lactulose, glucose, betaine, etc.) showed significant differences between the two groups. In milk, 122 metabolites were identified, and 20 metabolites (include urea, carnitine, acetate, butyrate, arabinitol, etc.) showed significant differences. Conclusion Our results show that different concentrate to forage feed ratios impact the metabolite levels in the serum and milk of lactating Holstein cows. A higher number of metabolites in milk, including those associated with milk fat synthesis and the presence of Escherichia coli in the rumen, differed between the two groups compared to that in the serum. The results of this study provide a useful insight into the metabolites associated with different concentrate to forge feed ratios in cows and may aid in the search for potential biomarkers for subacute ruminal acidosis.http://www.animbiosci.org/upload/pdf/ab-22-0486.pdfconcentratedairy cowforagemetaboliteproton nuclear magnetic resonanceruminal acidosis |
| spellingShingle | Hyun Sang Kim Jun Sik Eom Shin Ja Lee Youyoung Choi Seong Uk Jo Sang Suk Lee Eun Tae Kim Sung Sill Lee Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis Animal Bioscience concentrate dairy cow forage metabolite proton nuclear magnetic resonance ruminal acidosis |
| title | Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis |
| title_full | Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis |
| title_fullStr | Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis |
| title_full_unstemmed | Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis |
| title_short | Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis |
| title_sort | blood and milk metabolites of holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis |
| topic | concentrate dairy cow forage metabolite proton nuclear magnetic resonance ruminal acidosis |
| url | http://www.animbiosci.org/upload/pdf/ab-22-0486.pdf |
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