Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells
Abstract This study investigated the repair effects of three Astragalus polysaccharides (APSs) with different molecular weights (Mws) on injured human renal proximal tubular epithelial (HK-2) cells to reveal the effect of Mw of polysaccharide on cell repair. A damage model was established by injurin...
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| Format: | Article |
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Nature Portfolio
2019-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-019-46264-y |
| _version_ | 1819200322147450880 |
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| author | Jin Han Da Guo Xin-Yuan Sun Jian-Min Wang Jian-Ming Ouyang Bao-Song Gui |
| author_facet | Jin Han Da Guo Xin-Yuan Sun Jian-Min Wang Jian-Ming Ouyang Bao-Song Gui |
| author_sort | Jin Han |
| collection | DOAJ |
| description | Abstract This study investigated the repair effects of three Astragalus polysaccharides (APSs) with different molecular weights (Mws) on injured human renal proximal tubular epithelial (HK-2) cells to reveal the effect of Mw of polysaccharide on cell repair. A damage model was established by injuring HK-2 cells with 2.6 mM oxalate, and APS0, APS1, and APS2 with Mw of 11.03, 4.72, and 2.61 KDa were used to repair the damaged cells. After repair by APSs, the morphology of damaged HK-2 cells gradually returned to normal, the destruction of intercellular junctions recovered, intracellular reactive oxygen species production amount decreased, and their mitochondrial membrane potential increased. In addition, the cell cycle progression gradually normalized, lysosome integrity increased, and cell apoptotic rates obviously declined in the repaired cells. All three APSs could promote the expression of Keap1, Nrf2, SOD1, and CAT. In addition, the expression levels of inflammation markers containing MCP-1 and IL-6 decreased after APS repair. We deduced that APSs exert their repair function by activating the Nrf2–Keap1 signaling pathway and inhibiting inflammation. Among the APSs, APS1 with a moderate Mw provided the strongest repair effect. APSs may have a preventive effect on kidney stones. |
| first_indexed | 2024-12-23T03:30:23Z |
| format | Article |
| id | doaj.art-e65ef4efcbb5408ab982403efd15b4d5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T03:30:23Z |
| publishDate | 2019-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e65ef4efcbb5408ab982403efd15b4d52022-12-21T18:01:44ZengNature PortfolioScientific Reports2045-23222019-07-019111510.1038/s41598-019-46264-yRepair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 CellsJin Han0Da Guo1Xin-Yuan Sun2Jian-Min Wang3Jian-Ming Ouyang4Bao-Song Gui5Department of Nephrology, the Second Hospital of Xi’an Jiaotong UniversityInstitute of Biomineralization and Lithiasis Research, Jinan UniversityInstitute of Biomineralization and Lithiasis Research, Jinan UniversityInstitute of Biomineralization and Lithiasis Research, Jinan UniversityInstitute of Biomineralization and Lithiasis Research, Jinan UniversityDepartment of Nephrology, the Second Hospital of Xi’an Jiaotong UniversityAbstract This study investigated the repair effects of three Astragalus polysaccharides (APSs) with different molecular weights (Mws) on injured human renal proximal tubular epithelial (HK-2) cells to reveal the effect of Mw of polysaccharide on cell repair. A damage model was established by injuring HK-2 cells with 2.6 mM oxalate, and APS0, APS1, and APS2 with Mw of 11.03, 4.72, and 2.61 KDa were used to repair the damaged cells. After repair by APSs, the morphology of damaged HK-2 cells gradually returned to normal, the destruction of intercellular junctions recovered, intracellular reactive oxygen species production amount decreased, and their mitochondrial membrane potential increased. In addition, the cell cycle progression gradually normalized, lysosome integrity increased, and cell apoptotic rates obviously declined in the repaired cells. All three APSs could promote the expression of Keap1, Nrf2, SOD1, and CAT. In addition, the expression levels of inflammation markers containing MCP-1 and IL-6 decreased after APS repair. We deduced that APSs exert their repair function by activating the Nrf2–Keap1 signaling pathway and inhibiting inflammation. Among the APSs, APS1 with a moderate Mw provided the strongest repair effect. APSs may have a preventive effect on kidney stones.https://doi.org/10.1038/s41598-019-46264-y |
| spellingShingle | Jin Han Da Guo Xin-Yuan Sun Jian-Min Wang Jian-Ming Ouyang Bao-Song Gui Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells Scientific Reports |
| title | Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells |
| title_full | Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells |
| title_fullStr | Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells |
| title_full_unstemmed | Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells |
| title_short | Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells |
| title_sort | repair effects of astragalus polysaccharides with different molecular weights on oxidatively damaged hk 2 cells |
| url | https://doi.org/10.1038/s41598-019-46264-y |
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