A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation
Abstract Subcutaneous islet transplantation is a promising treatment for severe diabetes; however, poor engraftment hinders its prevalence. We previously reported that a recombinant peptide (RCP) enhances subcutaneous islet engraftment. However, it is impractical for clinical use because RCP must be...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39212-4 |
| _version_ | 1797769607660961792 |
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| author | Norifumi Kanai Akiko Inagaki Yasuhiro Nakamura Takehiro Imura Hiroaki Mitsugashira Ryusuke Saito Shigehito Miyagi Kimiko Watanabe Takashi Kamei Michiaki Unno Yasuhiko Tabata Masafumi Goto |
| author_facet | Norifumi Kanai Akiko Inagaki Yasuhiro Nakamura Takehiro Imura Hiroaki Mitsugashira Ryusuke Saito Shigehito Miyagi Kimiko Watanabe Takashi Kamei Michiaki Unno Yasuhiko Tabata Masafumi Goto |
| author_sort | Norifumi Kanai |
| collection | DOAJ |
| description | Abstract Subcutaneous islet transplantation is a promising treatment for severe diabetes; however, poor engraftment hinders its prevalence. We previously reported that a recombinant peptide (RCP) enhances subcutaneous islet engraftment. However, it is impractical for clinical use because RCP must be removed when transplanting islets. We herein investigated whether a novel bioabsorbable gelatin hydrogel nonwoven fabric (GHNF) could improve subcutaneous islet engraftment. A silicon spacer with or without GHNF was implanted into the subcutaneous space of diabetic mice. Syngeneic islets were transplanted into the pretreated space or intraportally (Ipo group). Blood glucose, intraperitoneal glucose tolerance, immunohistochemistry, CT angiography and gene expression were evaluated. The cure rate and glucose tolerance of the GHNF group were significantly better than in the control and Ipo groups (p < 0.01, p < 0.05, respectively). In the GHNF group, a limited increase of vWF-positive vessels was detected in the islet capsule, whereas laminin (p < 0.05), collagen III and IV were considerably enhanced. TaqMan arrays revealed a significant upregulation of 19 target genes (including insulin-like growth factor-2) in the pretreated space. GHNF markedly improved the subcutaneous islet transplantation outcomes, likely due to ECM compensation and protection of islet function by various growth factors, rather than enhanced neovascularization. |
| first_indexed | 2024-03-12T21:10:27Z |
| format | Article |
| id | doaj.art-e6628cebd77a4e26bd5fd1d7e4df1d27 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T21:10:27Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e6628cebd77a4e26bd5fd1d7e4df1d272023-07-30T11:12:34ZengNature PortfolioScientific Reports2045-23222023-07-0113111210.1038/s41598-023-39212-4A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantationNorifumi Kanai0Akiko Inagaki1Yasuhiro Nakamura2Takehiro Imura3Hiroaki Mitsugashira4Ryusuke Saito5Shigehito Miyagi6Kimiko Watanabe7Takashi Kamei8Michiaki Unno9Yasuhiko Tabata10Masafumi Goto11Department of Surgery, Tohoku University Graduate School of MedicineDivision of Transplantation and Regenerative Medicine, Tohoku University Graduate School of MedicineDivision of Pathology, Graduate School of Medicine, Tohoku Medical and Pharmaceutical UniversityDivision of Transplantation and Regenerative Medicine, Tohoku University Graduate School of MedicineDepartment of Surgery, Tohoku University Graduate School of MedicineDepartment of Surgery, Tohoku University Graduate School of MedicineDepartment of Surgery, Tohoku University Graduate School of MedicineDivision of Transplantation and Regenerative Medicine, Tohoku University Graduate School of MedicineDepartment of Surgery, Tohoku University Graduate School of MedicineDepartment of Surgery, Tohoku University Graduate School of MedicineLaboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Life and Medical Sciences (LiMe), Kyoto UniversityDepartment of Surgery, Tohoku University Graduate School of MedicineAbstract Subcutaneous islet transplantation is a promising treatment for severe diabetes; however, poor engraftment hinders its prevalence. We previously reported that a recombinant peptide (RCP) enhances subcutaneous islet engraftment. However, it is impractical for clinical use because RCP must be removed when transplanting islets. We herein investigated whether a novel bioabsorbable gelatin hydrogel nonwoven fabric (GHNF) could improve subcutaneous islet engraftment. A silicon spacer with or without GHNF was implanted into the subcutaneous space of diabetic mice. Syngeneic islets were transplanted into the pretreated space or intraportally (Ipo group). Blood glucose, intraperitoneal glucose tolerance, immunohistochemistry, CT angiography and gene expression were evaluated. The cure rate and glucose tolerance of the GHNF group were significantly better than in the control and Ipo groups (p < 0.01, p < 0.05, respectively). In the GHNF group, a limited increase of vWF-positive vessels was detected in the islet capsule, whereas laminin (p < 0.05), collagen III and IV were considerably enhanced. TaqMan arrays revealed a significant upregulation of 19 target genes (including insulin-like growth factor-2) in the pretreated space. GHNF markedly improved the subcutaneous islet transplantation outcomes, likely due to ECM compensation and protection of islet function by various growth factors, rather than enhanced neovascularization.https://doi.org/10.1038/s41598-023-39212-4 |
| spellingShingle | Norifumi Kanai Akiko Inagaki Yasuhiro Nakamura Takehiro Imura Hiroaki Mitsugashira Ryusuke Saito Shigehito Miyagi Kimiko Watanabe Takashi Kamei Michiaki Unno Yasuhiko Tabata Masafumi Goto A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation Scientific Reports |
| title | A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation |
| title_full | A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation |
| title_fullStr | A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation |
| title_full_unstemmed | A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation |
| title_short | A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation |
| title_sort | gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation |
| url | https://doi.org/10.1038/s41598-023-39212-4 |
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