A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans
Based on serial sectioning, focused ion beam scanning electron microscopy (FIB/SEM), and electron tomography, we depict in detail the highly unusual anatomy of the marine hyperthermophilic crenarchaeon, Ignicoccus hospitalis. Our data support a complex and dynamic endomembrane system consisting of c...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01072/full |
| _version_ | 1819157528988090368 |
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| author | Thomas Heimerl Jennifer Flechsler Carolin Pickl Veronika Heinz Benjamin Salecker Josef Zweck Gerhard Wanner Stefan Geimer Rachel Y. Samson Stephen D. Bell Harald Huber Reinhard Wirth Louie Wurch Louie Wurch Mircea Podar Mircea Podar Reinhard Rachel |
| author_facet | Thomas Heimerl Jennifer Flechsler Carolin Pickl Veronika Heinz Benjamin Salecker Josef Zweck Gerhard Wanner Stefan Geimer Rachel Y. Samson Stephen D. Bell Harald Huber Reinhard Wirth Louie Wurch Louie Wurch Mircea Podar Mircea Podar Reinhard Rachel |
| author_sort | Thomas Heimerl |
| collection | DOAJ |
| description | Based on serial sectioning, focused ion beam scanning electron microscopy (FIB/SEM), and electron tomography, we depict in detail the highly unusual anatomy of the marine hyperthermophilic crenarchaeon, Ignicoccus hospitalis. Our data support a complex and dynamic endomembrane system consisting of cytoplasmic protrusions, and with secretory function. Moreover, we reveal that the cytoplasm of the putative archaeal ectoparasite Nanoarchaeum equitans can get in direct contact with this endomembrane system, complementing and explaining recent proteomic, transcriptomic and metabolomic data on this inter-archaeal relationship. In addition, we identified a matrix of filamentous structures and/or tethers in the voluminous inter-membrane compartment (IMC) of I. hospitalis, which might be responsible for membrane dynamics. Overall, this unusual cellular compartmentalization, ultrastructure and dynamics in an archaeon that belongs to the recently proposed TACK superphylum prompts speculation that the eukaryotic endomembrane system might originate from Archaea. |
| first_indexed | 2024-12-22T16:10:12Z |
| format | Article |
| id | doaj.art-e66308ba27d147eaadbd019c4688c1ba |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T16:10:12Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e66308ba27d147eaadbd019c4688c1ba2022-12-21T18:20:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-06-01810.3389/fmicb.2017.01072256456A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitansThomas Heimerl0Jennifer Flechsler1Carolin Pickl2Veronika Heinz3Benjamin Salecker4Josef Zweck5Gerhard Wanner6Stefan Geimer7Rachel Y. Samson8Stephen D. Bell9Harald Huber10Reinhard Wirth11Louie Wurch12Louie Wurch13Mircea Podar14Mircea Podar15Reinhard Rachel16LOEWE Research Center for Synthetic Microbiology (SYNMIKRO), Philipps University of MarburgMarburg, GermanyPlant Development and Electron Microscopy, Biocenter LMUMunich, GermanyPlant Development and Electron Microscopy, Biocenter LMUMunich, GermanyCenter for Electron Microscopy, University of RegensburgRegensburg, GermanyCenter for Electron Microscopy, University of RegensburgRegensburg, GermanyInstitute of Experimental and Applied Physics, University of RegensburgRegensburg, GermanyPlant Development and Electron Microscopy, Biocenter LMUMunich, GermanyCell Biology and Electron Microscopy, University of BayreuthBayreuth, GermanyMolecular and Cellular Biochemistry Department, Indiana UniversityBloomington, IN, United StatesMolecular and Cellular Biochemistry Department, Indiana UniversityBloomington, IN, United StatesMicrobiology Department, University of RegensburgRegensburg, GermanyMicrobiology Department, University of RegensburgRegensburg, GermanyBiosciences Division, Oak Ridge National LaboratoryOak Ridge, TN, United StatesMicrobiology Department, University of TennesseeKnoxville, TN, United StatesBiosciences Division, Oak Ridge National LaboratoryOak Ridge, TN, United StatesMicrobiology Department, University of TennesseeKnoxville, TN, United StatesCenter for Electron Microscopy, University of RegensburgRegensburg, GermanyBased on serial sectioning, focused ion beam scanning electron microscopy (FIB/SEM), and electron tomography, we depict in detail the highly unusual anatomy of the marine hyperthermophilic crenarchaeon, Ignicoccus hospitalis. Our data support a complex and dynamic endomembrane system consisting of cytoplasmic protrusions, and with secretory function. Moreover, we reveal that the cytoplasm of the putative archaeal ectoparasite Nanoarchaeum equitans can get in direct contact with this endomembrane system, complementing and explaining recent proteomic, transcriptomic and metabolomic data on this inter-archaeal relationship. In addition, we identified a matrix of filamentous structures and/or tethers in the voluminous inter-membrane compartment (IMC) of I. hospitalis, which might be responsible for membrane dynamics. Overall, this unusual cellular compartmentalization, ultrastructure and dynamics in an archaeon that belongs to the recently proposed TACK superphylum prompts speculation that the eukaryotic endomembrane system might originate from Archaea.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01072/fullArchaeaultrastructure3DeukaryogenesisFIB/SEMelectron tomography |
| spellingShingle | Thomas Heimerl Jennifer Flechsler Carolin Pickl Veronika Heinz Benjamin Salecker Josef Zweck Gerhard Wanner Stefan Geimer Rachel Y. Samson Stephen D. Bell Harald Huber Reinhard Wirth Louie Wurch Louie Wurch Mircea Podar Mircea Podar Reinhard Rachel A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans Frontiers in Microbiology Archaea ultrastructure 3D eukaryogenesis FIB/SEM electron tomography |
| title | A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans |
| title_full | A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans |
| title_fullStr | A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans |
| title_full_unstemmed | A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans |
| title_short | A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans |
| title_sort | complex endomembrane system in the archaeon ignicoccus hospitalis tapped by nanoarchaeum equitans |
| topic | Archaea ultrastructure 3D eukaryogenesis FIB/SEM electron tomography |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01072/full |
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