| Summary: | The complete mitogenomes of four species, <i>Neptis thisbe</i>, <i>Neptis obscurior</i>, <i>Athyma zeroca</i>, and <i>Aldania raddei</i>, were sequenced with sizes ranging from 15,172 bp (<i>N. obscurior</i>) to 16,348 bp (<i>Al. raddei</i>). All four mitogenomes display similar nucleotide content and codon usage of protein-coding genes (PCGs). Typical cloverleaf secondary structures are identified in 21 tRNA genes, while <i>trnS1</i> (AGN) lacks the dihydrouridine (DHC) arm. The gene orientation and arrangement of the four mitogenomes are similar to that of other typical mitogenomes of Lepidoptera. The Ka/Ks ratio of 13 PCGs among 58 Limenitidinae species reveals that <i>cox1</i> had the slowest evolutionary rate, while <i>atp8</i> and <i>nad6</i> exhibited a higher evolutionary rate. The phylogenetic analysis reveals that tribe-levels are well-supported monophyletic groups. Additionally, Maximum Likelihood analysis recovered the relationship (Parthenini + ((Chalingini + (Cymothoini + Neptini)) + (Adoliadini + Limenitidini))). However, a Bayesian analysis based on the same dataset recovered the relationship (Parthenini + (Adoliadini + ((Cymothoini + Neptini) + (Chalingini + Limenitidini)))). These results will offer valuable data for the future study of the phylogenetic relationships for Limenitidinae.
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