Anti-cancer activity and apoptosis inducing effect of methanolic extract of <i>Cordia dichotoma</i> against human cancer cell line
MTT assay and DAPI staining test were performed to evaluate anticancer potential and to assess apoptosis inducing effect of methanolic extract of Cordia dichotoma leaves (MECD) against human cervical cancer cell line (HeLa). Changes in MMP and intracellular ROS level were also assessed by JC-1 and D...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2015-01-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/20883 |
| _version_ | 1818060397148635136 |
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| author | Md. Azizur Rahman Arshad Hussain |
| author_facet | Md. Azizur Rahman Arshad Hussain |
| author_sort | Md. Azizur Rahman |
| collection | DOAJ |
| description | MTT assay and DAPI staining test were performed to evaluate anticancer potential and to assess apoptosis inducing effect of methanolic extract of Cordia dichotoma leaves (MECD) against human cervical cancer cell line (HeLa). Changes in MMP and intracellular ROS level were also assessed by JC-1 and DCFH-DA staining. Total phenolic contents were determined by colorimetric principle. Levels of statistical significance were determined by one-way analysis of variance followed by Dunnetts posttest. Results showed that MECD with obtained IC50 of 202 µg/mL inhibited in vitro proliferation of human cervical cancer cells and induced apoptosis indicating its promising anticancer activity as compared to the standard tamoxifen with obtained IC50 of 48 µg/mL. Total phenolic contents was found to be 176.5 mg GAE/g dried extract. It was concluded that MECD possess promising anticancer activity and induce apoptosis. |
| first_indexed | 2024-12-10T13:31:46Z |
| format | Article |
| id | doaj.art-e66f8b5d8b0e4d6ca83f850d75858bff |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-10T13:31:46Z |
| publishDate | 2015-01-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-e66f8b5d8b0e4d6ca83f850d75858bff2022-12-22T01:46:56ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882015-01-01101Anti-cancer activity and apoptosis inducing effect of methanolic extract of <i>Cordia dichotoma</i> against human cancer cell lineMd. Azizur Rahman0Arshad Hussain1UP-CST Sponsored Project Lab, Faculty of Pharmacy, Integral University, Lucknow 226026, Uttar PradeshUP-CST Sponsored Project Lab, Faculty of Pharmacy, Integral University, Lucknow 226026, Uttar PradeshMTT assay and DAPI staining test were performed to evaluate anticancer potential and to assess apoptosis inducing effect of methanolic extract of Cordia dichotoma leaves (MECD) against human cervical cancer cell line (HeLa). Changes in MMP and intracellular ROS level were also assessed by JC-1 and DCFH-DA staining. Total phenolic contents were determined by colorimetric principle. Levels of statistical significance were determined by one-way analysis of variance followed by Dunnetts posttest. Results showed that MECD with obtained IC50 of 202 µg/mL inhibited in vitro proliferation of human cervical cancer cells and induced apoptosis indicating its promising anticancer activity as compared to the standard tamoxifen with obtained IC50 of 48 µg/mL. Total phenolic contents was found to be 176.5 mg GAE/g dried extract. It was concluded that MECD possess promising anticancer activity and induce apoptosis.https://www.banglajol.info/index.php/BJP/article/view/20883Anticancer activityApoptosisCordia dichotomaMedicinal plantMTT assay |
| spellingShingle | Md. Azizur Rahman Arshad Hussain Anti-cancer activity and apoptosis inducing effect of methanolic extract of <i>Cordia dichotoma</i> against human cancer cell line Bangladesh Journal of Pharmacology Anticancer activity Apoptosis Cordia dichotoma Medicinal plant MTT assay |
| title | Anti-cancer activity and apoptosis inducing effect of methanolic extract of <i>Cordia dichotoma</i> against human cancer cell line |
| title_full | Anti-cancer activity and apoptosis inducing effect of methanolic extract of <i>Cordia dichotoma</i> against human cancer cell line |
| title_fullStr | Anti-cancer activity and apoptosis inducing effect of methanolic extract of <i>Cordia dichotoma</i> against human cancer cell line |
| title_full_unstemmed | Anti-cancer activity and apoptosis inducing effect of methanolic extract of <i>Cordia dichotoma</i> against human cancer cell line |
| title_short | Anti-cancer activity and apoptosis inducing effect of methanolic extract of <i>Cordia dichotoma</i> against human cancer cell line |
| title_sort | anti cancer activity and apoptosis inducing effect of methanolic extract of i cordia dichotoma i against human cancer cell line |
| topic | Anticancer activity Apoptosis Cordia dichotoma Medicinal plant MTT assay |
| url | https://www.banglajol.info/index.php/BJP/article/view/20883 |
| work_keys_str_mv | AT mdazizurrahman anticanceractivityandapoptosisinducingeffectofmethanolicextractoficordiadichotomaiagainsthumancancercellline AT arshadhussain anticanceractivityandapoptosisinducingeffectofmethanolicextractoficordiadichotomaiagainsthumancancercellline |