| Summary: | Transient receptor potential mucolipin (TRPML) protein in flies plays a pivotal role in Ca<sup>2+</sup> ions release, resulting in membrane trafficking, autophagy and ion homeostasis. However, to date, the characterization of TRPML in agricultural pests remains unknown. Here, we firstly reported the TRPML of a destructive pest of gramineous crops, <i>Laodelphax striatellus</i>. The <i>L</i>. <i>striatellus</i> TRPML (Ls-TRPML) has a 1818 bp open reading frame, encoding 605 amino acid. TRPML in agricultural pests is evolutionarily conserved, and the expression of Ls-TRPML is predominately higher in the ovary than in other organs of <i>L</i>. <i>striatellus</i> at the transcript and protein level. The Bac–Bac system showed that Ls-TRPML localized in the plasma membrane, nuclear membrane and nucleus and co-localized with lysosome in <i>Spodoptera frugiperda</i> cells. The immunofluorescence microscopy analysis showed that Ls-TRPML localized in the cytoplasm and around the nuclei of the intestine cells or ovary follicular cells of <i>L</i>. <i>striatellus</i>. The results from the lipid-binding assay revealed that Ls-TRPML strongly bound to phosphatidylinositol-3,5-bisphosphate, as compared with other phosphoinositides. Overall, our results helped is identify and characterize the TRPML protein of <i>L</i>. <i>striatellus</i>, shedding light on the function of TRPML in multiple cellular processes in agricultural pests.
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