Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and Outcomes
Cartilage injury defects in animals and humans result in the development of osteoarthritis and the progression of joint deterioration. Cell isolation from equine hyaline cartilage and evaluation of their ability to repair equine joint cartilage injuries establish a new experimental protocol for an a...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/6/1602 |
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| author | Fernando Canonici Cristiano Cocumelli Antonella Cersini Daniele Marcoccia Alessia Zepparoni Annalisa Altigeri Daniela Caciolo Cristina Roncoroni Valentina Monteleone Elisa Innocenzi Cristian Alimonti Paola Ghisellini Cristina Rando Eugenia Pechkova Roberto Eggenhöffner Maria Teresa Scicluna Katia Barbaro |
| author_facet | Fernando Canonici Cristiano Cocumelli Antonella Cersini Daniele Marcoccia Alessia Zepparoni Annalisa Altigeri Daniela Caciolo Cristina Roncoroni Valentina Monteleone Elisa Innocenzi Cristian Alimonti Paola Ghisellini Cristina Rando Eugenia Pechkova Roberto Eggenhöffner Maria Teresa Scicluna Katia Barbaro |
| author_sort | Fernando Canonici |
| collection | DOAJ |
| description | Cartilage injury defects in animals and humans result in the development of osteoarthritis and the progression of joint deterioration. Cell isolation from equine hyaline cartilage and evaluation of their ability to repair equine joint cartilage injuries establish a new experimental protocol for an alternative approach to osteochondral lesions treatment. Chondrocytes (CCs), isolated from the autologous cartilage of the trachea, grown in the laboratory, and subsequently arthroscopically implanted into the lesion site, were used to regenerate a chondral lesion of the carpal joint of a horse. Biopsies of the treated cartilage taken after 8 and 13 months of implantation for histological and immunohistochemical evaluation of the tissue demonstrate that the tissue was still immature 8 months after implantation, while at 13 months it was organized almost similarly to the original hyaline cartilage. Finally, a tissue perfectly comparable to native articular cartilage was detected 24 months after implantation. Histological investigations demonstrate the progressive maturation of the hyaline cartilage at the site of the lesion. The hyaline type of tracheal cartilage, used as a source of CCs, allows for the repair of joint cartilage injuries through the neosynthesis of hyaline cartilage that presents characteristics identical to the articular cartilage of the original tissue. |
| first_indexed | 2024-03-11T02:45:14Z |
| format | Article |
| id | doaj.art-e6714869149e4e6c869a7f1c7c4b0561 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T02:45:14Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-e6714869149e4e6c869a7f1c7c4b05612023-11-18T09:25:37ZengMDPI AGBiomedicines2227-90592023-05-01116160210.3390/biomedicines11061602Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and OutcomesFernando Canonici0Cristiano Cocumelli1Antonella Cersini2Daniele Marcoccia3Alessia Zepparoni4Annalisa Altigeri5Daniela Caciolo6Cristina Roncoroni7Valentina Monteleone8Elisa Innocenzi9Cristian Alimonti10Paola Ghisellini11Cristina Rando12Eugenia Pechkova13Roberto Eggenhöffner14Maria Teresa Scicluna15Katia Barbaro16Equine Practice s.r.l., Campagnano, Strada Valle del Baccano 80, 00063 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyDepartment of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, 16132 Genoa, ItalyDepartment of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, 16132 Genoa, ItalyConsorzio Interuniversitario INBB, Viale delle Medaglie d’Oro 305, 00136 Rome, ItalyDepartment of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, 16132 Genoa, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyCartilage injury defects in animals and humans result in the development of osteoarthritis and the progression of joint deterioration. Cell isolation from equine hyaline cartilage and evaluation of their ability to repair equine joint cartilage injuries establish a new experimental protocol for an alternative approach to osteochondral lesions treatment. Chondrocytes (CCs), isolated from the autologous cartilage of the trachea, grown in the laboratory, and subsequently arthroscopically implanted into the lesion site, were used to regenerate a chondral lesion of the carpal joint of a horse. Biopsies of the treated cartilage taken after 8 and 13 months of implantation for histological and immunohistochemical evaluation of the tissue demonstrate that the tissue was still immature 8 months after implantation, while at 13 months it was organized almost similarly to the original hyaline cartilage. Finally, a tissue perfectly comparable to native articular cartilage was detected 24 months after implantation. Histological investigations demonstrate the progressive maturation of the hyaline cartilage at the site of the lesion. The hyaline type of tracheal cartilage, used as a source of CCs, allows for the repair of joint cartilage injuries through the neosynthesis of hyaline cartilage that presents characteristics identical to the articular cartilage of the original tissue.https://www.mdpi.com/2227-9059/11/6/1602articular cartilage repairregenerative medicinehorsehyaline cartilage |
| spellingShingle | Fernando Canonici Cristiano Cocumelli Antonella Cersini Daniele Marcoccia Alessia Zepparoni Annalisa Altigeri Daniela Caciolo Cristina Roncoroni Valentina Monteleone Elisa Innocenzi Cristian Alimonti Paola Ghisellini Cristina Rando Eugenia Pechkova Roberto Eggenhöffner Maria Teresa Scicluna Katia Barbaro Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and Outcomes Biomedicines articular cartilage repair regenerative medicine horse hyaline cartilage |
| title | Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and Outcomes |
| title_full | Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and Outcomes |
| title_fullStr | Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and Outcomes |
| title_full_unstemmed | Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and Outcomes |
| title_short | Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and Outcomes |
| title_sort | articular cartilage regeneration by hyaline chondrocytes a case study in equine model and outcomes |
| topic | articular cartilage repair regenerative medicine horse hyaline cartilage |
| url | https://www.mdpi.com/2227-9059/11/6/1602 |
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