Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment
In this study, the by-products of Chinese softshell turtle processing were used as raw materials, which provided the possibility to improve the extraction efficiency of Chinese softshell turtle oil and make it high-value utilization to provide a new source of profit for production enterprises. The e...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-11-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030035 |
| _version_ | 1811308350008197120 |
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| author | Qianyi SHAN Yueming SHEN Mingxing ZHANG Jiacheng TANG Yanjie CHEN Jianqiang BAO |
| author_facet | Qianyi SHAN Yueming SHEN Mingxing ZHANG Jiacheng TANG Yanjie CHEN Jianqiang BAO |
| author_sort | Qianyi SHAN |
| collection | DOAJ |
| description | In this study, the by-products of Chinese softshell turtle processing were used as raw materials, which provided the possibility to improve the extraction efficiency of Chinese softshell turtle oil and make it high-value utilization to provide a new source of profit for production enterprises. The extraction experiment of turtle oil was carried out by using ultrasonic combined with complex enzymatic hydrolysis (The pH of enzymatic hydrolysis was controlled at 7.5~8.5), and the extraction parameters were optimized by single factor experiments combined with orthogonal experiment, and the optimal extraction process conditions of ultrasonic-complex enzymatic hydrolysis method were obtained: The ratio of solid to liquid was 1:1.5 (g/mL), the amount of protease was 2.4%, the ratio of enzyme was 1:1.5 (neutral protease:alkaline protease), the enzymatic hydrolysis time was 2 h, the enzymatic hydrolysis temperature was 65 ℃, the ultrasonic power was 180 W, and the ultrasonic time was 30 min. The extraction rate of Chinese softshell turtle oil was 81.65%±0.62%. After the Chinese softshell turtle oil was refined, its physical and chemical indicators were all in line with the national aquatic industry standard (SC/T 3502-2016) refined fish oil first-class standard. At the same time, fatty acid composition analysis showed that the refined Chinese softshell turtle oil contained a total of 28 kinds of fatty acids, of which saturated fatty acids accounted for 23.81% of the total, monounsaturated fatty acids accounted for 46.94% of the total, polyunsaturated fatty acids accounted for 26.38% of the total, DHA and EPA content accounted for 10.05% of the total. |
| first_indexed | 2024-04-13T09:21:38Z |
| format | Article |
| id | doaj.art-e6723780a8ff421dbaa562ded32f4ed0 |
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| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:21:38Z |
| publishDate | 2022-11-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-e6723780a8ff421dbaa562ded32f4ed02022-12-22T02:52:36ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-11-01432225626410.13386/j.issn1002-0306.20220300352022030035-22Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal ExperimentQianyi SHAN0Yueming SHEN1Mingxing ZHANG2Jiacheng TANG3Yanjie CHEN4Jianqiang BAO5College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaNingbo Mingfeng Fishery Co., Ltd., Ningbo 315464, ChinaNingbo Mingfeng Fishery Co., Ltd., Ningbo 315464, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaIn this study, the by-products of Chinese softshell turtle processing were used as raw materials, which provided the possibility to improve the extraction efficiency of Chinese softshell turtle oil and make it high-value utilization to provide a new source of profit for production enterprises. The extraction experiment of turtle oil was carried out by using ultrasonic combined with complex enzymatic hydrolysis (The pH of enzymatic hydrolysis was controlled at 7.5~8.5), and the extraction parameters were optimized by single factor experiments combined with orthogonal experiment, and the optimal extraction process conditions of ultrasonic-complex enzymatic hydrolysis method were obtained: The ratio of solid to liquid was 1:1.5 (g/mL), the amount of protease was 2.4%, the ratio of enzyme was 1:1.5 (neutral protease:alkaline protease), the enzymatic hydrolysis time was 2 h, the enzymatic hydrolysis temperature was 65 ℃, the ultrasonic power was 180 W, and the ultrasonic time was 30 min. The extraction rate of Chinese softshell turtle oil was 81.65%±0.62%. After the Chinese softshell turtle oil was refined, its physical and chemical indicators were all in line with the national aquatic industry standard (SC/T 3502-2016) refined fish oil first-class standard. At the same time, fatty acid composition analysis showed that the refined Chinese softshell turtle oil contained a total of 28 kinds of fatty acids, of which saturated fatty acids accounted for 23.81% of the total, monounsaturated fatty acids accounted for 46.94% of the total, polyunsaturated fatty acids accounted for 26.38% of the total, DHA and EPA content accounted for 10.05% of the total.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030035by-products of chinese softshell turtle processingultrasonic wavecompound enzymeoil extractionprocess optimizationfatty acids |
| spellingShingle | Qianyi SHAN Yueming SHEN Mingxing ZHANG Jiacheng TANG Yanjie CHEN Jianqiang BAO Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment Shipin gongye ke-ji by-products of chinese softshell turtle processing ultrasonic wave compound enzyme oil extraction process optimization fatty acids |
| title | Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment |
| title_full | Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment |
| title_fullStr | Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment |
| title_full_unstemmed | Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment |
| title_short | Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment |
| title_sort | optimization of ultrasonic compound enzyme hydrolysis method for extracting chinese softshell turtle oil by orthogonal experiment |
| topic | by-products of chinese softshell turtle processing ultrasonic wave compound enzyme oil extraction process optimization fatty acids |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030035 |
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