Saint Peter and Saint Paul Archipelago barcoded: Fish diversity in the remoteness and DNA barcodes reference library for metabarcoding monitoring
Abstract In order to monitor the effects of anthropogenic pressures in ecosystems, molecular techniques can be used to characterize species composition. Among molecular markers capable of identifying species, the cytochrome c oxidase I (COI) is the most used. However, new possibilities of biodiversi...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2022-10-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000300201&lng=en&tlng=en |
| _version_ | 1817980667148894208 |
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| author | Marcelo Merten Cruz Lilian Sander Hoffmann Thales R. O. de Freitas |
| author_facet | Marcelo Merten Cruz Lilian Sander Hoffmann Thales R. O. de Freitas |
| author_sort | Marcelo Merten Cruz |
| collection | DOAJ |
| description | Abstract In order to monitor the effects of anthropogenic pressures in ecosystems, molecular techniques can be used to characterize species composition. Among molecular markers capable of identifying species, the cytochrome c oxidase I (COI) is the most used. However, new possibilities of biodiversity profiling have become possible, in which molecular fragments of medium and short-length can now be analyzed in metabarcoding studies. Here, a survey of fishes from the Saint Peter and Saint Paul Archipelago was barcoded using the COI marker, which allowed the identification of 21 species. This paved the way to further investigate the fish biodiversity of the archipelago, transitioning from barcoding to metabarcoding analysis. As preparatory steps for future metabarcoding studies, the first extensive COI library of fishes listed for these islands was constructed and includes new data generated in this survey as well as previously available data, resulting in a final database with 9,183 sequences from 169 species and 63 families of fish. A new primer specifically designed for those fishes was tested in silico to amplify a region of 262 bp. The new approach should guarantee a reliable surveillance of the archipelago and can be used to generate policies that will enhance the archipelago’s protection. |
| first_indexed | 2024-04-13T22:56:17Z |
| format | Article |
| id | doaj.art-e67468f9c564413a8dfca46e7df4acc2 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-04-13T22:56:17Z |
| publishDate | 2022-10-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-e67468f9c564413a8dfca46e7df4acc22022-12-22T02:26:00ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852022-10-0145310.1590/1678-4685-gmb-2021-0349Saint Peter and Saint Paul Archipelago barcoded: Fish diversity in the remoteness and DNA barcodes reference library for metabarcoding monitoringMarcelo Merten Cruzhttps://orcid.org/0000-0001-8374-5144Lilian Sander HoffmannThales R. O. de Freitashttps://orcid.org/0000-0002-1019-9303Abstract In order to monitor the effects of anthropogenic pressures in ecosystems, molecular techniques can be used to characterize species composition. Among molecular markers capable of identifying species, the cytochrome c oxidase I (COI) is the most used. However, new possibilities of biodiversity profiling have become possible, in which molecular fragments of medium and short-length can now be analyzed in metabarcoding studies. Here, a survey of fishes from the Saint Peter and Saint Paul Archipelago was barcoded using the COI marker, which allowed the identification of 21 species. This paved the way to further investigate the fish biodiversity of the archipelago, transitioning from barcoding to metabarcoding analysis. As preparatory steps for future metabarcoding studies, the first extensive COI library of fishes listed for these islands was constructed and includes new data generated in this survey as well as previously available data, resulting in a final database with 9,183 sequences from 169 species and 63 families of fish. A new primer specifically designed for those fishes was tested in silico to amplify a region of 262 bp. The new approach should guarantee a reliable surveillance of the archipelago and can be used to generate policies that will enhance the archipelago’s protection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000300201&lng=en&tlng=enBiodiversityconservationDNA barcodingislandprimer |
| spellingShingle | Marcelo Merten Cruz Lilian Sander Hoffmann Thales R. O. de Freitas Saint Peter and Saint Paul Archipelago barcoded: Fish diversity in the remoteness and DNA barcodes reference library for metabarcoding monitoring Genetics and Molecular Biology Biodiversity conservation DNA barcoding island primer |
| title | Saint Peter and Saint Paul Archipelago barcoded: Fish diversity in the remoteness and DNA barcodes reference library for metabarcoding monitoring |
| title_full | Saint Peter and Saint Paul Archipelago barcoded: Fish diversity in the remoteness and DNA barcodes reference library for metabarcoding monitoring |
| title_fullStr | Saint Peter and Saint Paul Archipelago barcoded: Fish diversity in the remoteness and DNA barcodes reference library for metabarcoding monitoring |
| title_full_unstemmed | Saint Peter and Saint Paul Archipelago barcoded: Fish diversity in the remoteness and DNA barcodes reference library for metabarcoding monitoring |
| title_short | Saint Peter and Saint Paul Archipelago barcoded: Fish diversity in the remoteness and DNA barcodes reference library for metabarcoding monitoring |
| title_sort | saint peter and saint paul archipelago barcoded fish diversity in the remoteness and dna barcodes reference library for metabarcoding monitoring |
| topic | Biodiversity conservation DNA barcoding island primer |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000300201&lng=en&tlng=en |
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