Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers
RAPD and ISSR markers were used in the present study to determine genetic relationship and distance between some populations of Carum carvi. Amplification of genomic DNA all population using RAPD analysis yielded 126 fragments, where TIBMBA02 and TIBMBA08 had the minimum number of fragments (7) and...
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| Format: | Article |
| Language: | fas |
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Islamic Azad University - Gorgan Branch
2017-12-01
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| Series: | فیزیولوژی محیطی گیاهی |
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| Online Access: | https://ecophysiologi.gorgan.iau.ir/article_606049_98daed4895f5dce5316db185ee586a20.pdf |
| _version_ | 1797808233221455872 |
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| author | leila fahmideh Lila Jani pour Bahman Fazeli-Nasab |
| author_facet | leila fahmideh Lila Jani pour Bahman Fazeli-Nasab |
| author_sort | leila fahmideh |
| collection | DOAJ |
| description | RAPD and ISSR markers were used in the present study to determine genetic relationship and distance between some populations of Carum carvi. Amplification of genomic DNA all population using RAPD analysis yielded 126 fragments, where TIBMBA02 and TIBMBA08 had the minimum number of fragments (7) and TIBMBC05 had the maximum number of fragments, (22). ISSR marker yielded 79 fragments, where UBC112 and UBC809had the minimum (1) and maximum (15) fragments, respectively. The Diversity Index value of RAPD primers ranged from 0.8 (TIBMBA02) to 0.94 (TIBMBC05) and also Shanoon Diversity and Nei diversity were 0.57 and 0.38, respectively. In ISSR primers The Diversity Index value ranged from 0.0 (UBC112) to 0.92 (UBC809) and also Shanoon Diversity and Nei diversity were 0.57 and 0.39 respectively. According to ISSR and RAPD markers in combination, maximum genetic similarity (0.48) was observed between Kerman and Jandagh Carum carvi while the minimum similarity (0.115) was observed between Kerman and Neishabour Carum carvi with average 0.315. Also, cluster analysis segregated all populations into 3 groups according to geographical zone. |
| first_indexed | 2024-03-13T06:34:29Z |
| format | Article |
| id | doaj.art-e6763bbddb0d4a258302128dabe1254e |
| institution | Directory Open Access Journal |
| issn | 2423-7671 2783-4689 |
| language | fas |
| last_indexed | 2024-03-13T06:34:29Z |
| publishDate | 2017-12-01 |
| publisher | Islamic Azad University - Gorgan Branch |
| record_format | Article |
| series | فیزیولوژی محیطی گیاهی |
| spelling | doaj.art-e6763bbddb0d4a258302128dabe1254e2023-06-09T06:34:55ZfasIslamic Azad University - Gorgan Branchفیزیولوژی محیطی گیاهی2423-76712783-46892017-12-0112487891606049Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markersleila fahmideh0Lila Jani pour1Bahman Fazeli-Nasab2Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, IranDepartment of Plant Breeding and Biotechnology, University of Zabol, Zabol, IranAgricultural Research Center of Zabol University, Zabol, IranRAPD and ISSR markers were used in the present study to determine genetic relationship and distance between some populations of Carum carvi. Amplification of genomic DNA all population using RAPD analysis yielded 126 fragments, where TIBMBA02 and TIBMBA08 had the minimum number of fragments (7) and TIBMBC05 had the maximum number of fragments, (22). ISSR marker yielded 79 fragments, where UBC112 and UBC809had the minimum (1) and maximum (15) fragments, respectively. The Diversity Index value of RAPD primers ranged from 0.8 (TIBMBA02) to 0.94 (TIBMBC05) and also Shanoon Diversity and Nei diversity were 0.57 and 0.38, respectively. In ISSR primers The Diversity Index value ranged from 0.0 (UBC112) to 0.92 (UBC809) and also Shanoon Diversity and Nei diversity were 0.57 and 0.39 respectively. According to ISSR and RAPD markers in combination, maximum genetic similarity (0.48) was observed between Kerman and Jandagh Carum carvi while the minimum similarity (0.115) was observed between Kerman and Neishabour Carum carvi with average 0.315. Also, cluster analysis segregated all populations into 3 groups according to geographical zone.https://ecophysiologi.gorgan.iau.ir/article_606049_98daed4895f5dce5316db185ee586a20.pdfgenetic diversitycluster analysiscarum carvidiversity indexrapd and issr markers |
| spellingShingle | leila fahmideh Lila Jani pour Bahman Fazeli-Nasab Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers فیزیولوژی محیطی گیاهی genetic diversity cluster analysis carum carvi diversity index rapd and issr markers |
| title | Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers |
| title_full | Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers |
| title_fullStr | Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers |
| title_full_unstemmed | Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers |
| title_short | Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers |
| title_sort | genetic assessment of some populations of the medicinal plant caraway carum carvi using rapd and issr markers |
| topic | genetic diversity cluster analysis carum carvi diversity index rapd and issr markers |
| url | https://ecophysiologi.gorgan.iau.ir/article_606049_98daed4895f5dce5316db185ee586a20.pdf |
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