Occurrence, Antimicrobial Resistance and Molecular Diversity of <i>Enterococcus</i> <i>faecium</i> in Processed Pork Meat Products in Korea
Because <i>Enterococcus faecium</i> is an important nosocomial pathogen and sentinel organism for tracking antimicrobial resistance, information on the contamination and antimicrobial resistance patterns of <i>E. faecium</i> in food are essential to public health and food saf...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/9/1283 |
| _version_ | 1827706408687108096 |
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| author | Hyun Jung Kim Minseon Koo |
| author_facet | Hyun Jung Kim Minseon Koo |
| author_sort | Hyun Jung Kim |
| collection | DOAJ |
| description | Because <i>Enterococcus faecium</i> is an important nosocomial pathogen and sentinel organism for tracking antimicrobial resistance, information on the contamination and antimicrobial resistance patterns of <i>E. faecium</i> in food are essential to public health and food safety. We analyzed the occurrence of <i>E. faecium</i> in retail pork meat products (<i>n</i> = 124), and antimicrobial resistance of 30 <i>E. faecium</i> isolates were examined against 14 antimicrobials using the broth dilution test and disc diffusion test. Rep-PCR-based molecular diversity was also analyzed using Deviersilab. The highest contamination of enterococci was observed for minced pork meat but most of the <i>E. faecium</i> was isolated from meatball-type frozen pork meat products (FP). Incidences of antimicrobial-resistant <i>E. faecium</i> against erythromycin, clindamycin and nitrofurantoin were 80%, 50% and 20%, respectively. No vancomycin-resistant enterococci were analyzed. Rep-PCR showed distinctive clusters with a similarity ≥ 98%, consisting of 18 <i>E. faecium</i> isolates from FP manufactured in seven companies. The analyzed data on the contamination and antimicrobial resistance patterns combined with molecular typing can be useful to derive risk management of antimicrobial-resistant enterococci in food. |
| first_indexed | 2024-03-10T16:23:01Z |
| format | Article |
| id | doaj.art-e67690e7f3424db78410237419c130d0 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T16:23:01Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-e67690e7f3424db78410237419c130d02023-11-20T13:31:11ZengMDPI AGFoods2304-81582020-09-0199128310.3390/foods9091283Occurrence, Antimicrobial Resistance and Molecular Diversity of <i>Enterococcus</i> <i>faecium</i> in Processed Pork Meat Products in KoreaHyun Jung Kim0Minseon Koo1Research Group of Consumer Safety, Korea Food Research Institute, Wanju, Jeollabuk-do 55365, KoreaDepartment of Food Biotechnology, University of Science and Technology, Daejeon 34113, KoreaBecause <i>Enterococcus faecium</i> is an important nosocomial pathogen and sentinel organism for tracking antimicrobial resistance, information on the contamination and antimicrobial resistance patterns of <i>E. faecium</i> in food are essential to public health and food safety. We analyzed the occurrence of <i>E. faecium</i> in retail pork meat products (<i>n</i> = 124), and antimicrobial resistance of 30 <i>E. faecium</i> isolates were examined against 14 antimicrobials using the broth dilution test and disc diffusion test. Rep-PCR-based molecular diversity was also analyzed using Deviersilab. The highest contamination of enterococci was observed for minced pork meat but most of the <i>E. faecium</i> was isolated from meatball-type frozen pork meat products (FP). Incidences of antimicrobial-resistant <i>E. faecium</i> against erythromycin, clindamycin and nitrofurantoin were 80%, 50% and 20%, respectively. No vancomycin-resistant enterococci were analyzed. Rep-PCR showed distinctive clusters with a similarity ≥ 98%, consisting of 18 <i>E. faecium</i> isolates from FP manufactured in seven companies. The analyzed data on the contamination and antimicrobial resistance patterns combined with molecular typing can be useful to derive risk management of antimicrobial-resistant enterococci in food.https://www.mdpi.com/2304-8158/9/9/1283<i>Enterococcus faecium</i>processed pork meat productsantimicrobial resistancerep-PCRmolecular typing |
| spellingShingle | Hyun Jung Kim Minseon Koo Occurrence, Antimicrobial Resistance and Molecular Diversity of <i>Enterococcus</i> <i>faecium</i> in Processed Pork Meat Products in Korea Foods <i>Enterococcus faecium</i> processed pork meat products antimicrobial resistance rep-PCR molecular typing |
| title | Occurrence, Antimicrobial Resistance and Molecular Diversity of <i>Enterococcus</i> <i>faecium</i> in Processed Pork Meat Products in Korea |
| title_full | Occurrence, Antimicrobial Resistance and Molecular Diversity of <i>Enterococcus</i> <i>faecium</i> in Processed Pork Meat Products in Korea |
| title_fullStr | Occurrence, Antimicrobial Resistance and Molecular Diversity of <i>Enterococcus</i> <i>faecium</i> in Processed Pork Meat Products in Korea |
| title_full_unstemmed | Occurrence, Antimicrobial Resistance and Molecular Diversity of <i>Enterococcus</i> <i>faecium</i> in Processed Pork Meat Products in Korea |
| title_short | Occurrence, Antimicrobial Resistance and Molecular Diversity of <i>Enterococcus</i> <i>faecium</i> in Processed Pork Meat Products in Korea |
| title_sort | occurrence antimicrobial resistance and molecular diversity of i enterococcus i i faecium i in processed pork meat products in korea |
| topic | <i>Enterococcus faecium</i> processed pork meat products antimicrobial resistance rep-PCR molecular typing |
| url | https://www.mdpi.com/2304-8158/9/9/1283 |
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