A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasma pneumoniae based on MALDI-TOF MS
Summary: In this study, a multisite SNP genotyping and macrolide (ML) susceptibility gene test method for Mycoplasma pneumoniae (M. pneumoniae) was developed based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The detection limit of this method for n...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-05-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221004156 |
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| author | Fei Zhao Jianzhong Zhang Xuemei Wang Liyong Liu Jie Gong Zhixiang Zhai Lihua He Fanliang Meng Di Xiao |
| author_facet | Fei Zhao Jianzhong Zhang Xuemei Wang Liyong Liu Jie Gong Zhixiang Zhai Lihua He Fanliang Meng Di Xiao |
| author_sort | Fei Zhao |
| collection | DOAJ |
| description | Summary: In this study, a multisite SNP genotyping and macrolide (ML) susceptibility gene test method for Mycoplasma pneumoniae (M. pneumoniae) was developed based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The detection limit of this method for nucleic acids was 102 -103 copies/reaction. Six SNP site-based genotyping and 3 ML susceptibility sites could be detected simultaneously based on multiplex PCR and mass probe. Using the method constructed in this study, 141 Chinese clinical isolates were divided into 8 SNP types. All the SNP test results for the ML susceptibility gene were in line with those of the 23S rRNA sequencing results. With this method, the multisite SNP genotyping and ML susceptibility determination of M. pneumoniae can be completed simultaneously in one test, which greatly reduces the workload and cost, improves the genotyping ability of M. pneumoniae and deserves clinical application. |
| first_indexed | 2024-12-17T00:38:52Z |
| format | Article |
| id | doaj.art-e678f61be244409391aac6429fa87b3e |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-17T00:38:52Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-e678f61be244409391aac6429fa87b3e2022-12-21T22:10:05ZengElsevieriScience2589-00422021-05-01245102447A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasma pneumoniae based on MALDI-TOF MSFei Zhao0Jianzhong Zhang1Xuemei Wang2Liyong Liu3Jie Gong4Zhixiang Zhai5Lihua He6Fanliang Meng7Di Xiao8National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, ChinaIntelligene Biosystems (Qingdao) Co., Ltd, Qingdao, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, ChinaIntelligene Biosystems (Qingdao) Co., Ltd, Qingdao, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, China; Corresponding authorSummary: In this study, a multisite SNP genotyping and macrolide (ML) susceptibility gene test method for Mycoplasma pneumoniae (M. pneumoniae) was developed based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The detection limit of this method for nucleic acids was 102 -103 copies/reaction. Six SNP site-based genotyping and 3 ML susceptibility sites could be detected simultaneously based on multiplex PCR and mass probe. Using the method constructed in this study, 141 Chinese clinical isolates were divided into 8 SNP types. All the SNP test results for the ML susceptibility gene were in line with those of the 23S rRNA sequencing results. With this method, the multisite SNP genotyping and ML susceptibility determination of M. pneumoniae can be completed simultaneously in one test, which greatly reduces the workload and cost, improves the genotyping ability of M. pneumoniae and deserves clinical application.http://www.sciencedirect.com/science/article/pii/S2589004221004156MicrobiologyMicrobial GeneticsBiotechnology |
| spellingShingle | Fei Zhao Jianzhong Zhang Xuemei Wang Liyong Liu Jie Gong Zhixiang Zhai Lihua He Fanliang Meng Di Xiao A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasma pneumoniae based on MALDI-TOF MS iScience Microbiology Microbial Genetics Biotechnology |
| title | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasma pneumoniae based on MALDI-TOF MS |
| title_full | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasma pneumoniae based on MALDI-TOF MS |
| title_fullStr | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasma pneumoniae based on MALDI-TOF MS |
| title_full_unstemmed | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasma pneumoniae based on MALDI-TOF MS |
| title_short | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasma pneumoniae based on MALDI-TOF MS |
| title_sort | multisite snp genotyping and macrolide susceptibility gene method for mycoplasma pneumoniae based on maldi tof ms |
| topic | Microbiology Microbial Genetics Biotechnology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221004156 |
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