Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients
Celiac disease (CD) is an autoimmune enteropathy. Peroxiredoxins (PRDXs) are powerful antioxidant enzymes having an important role in significant cellular pathways including cell survival, apoptosis, and inflammation. This study aimed at investigating the expression levels of all PRDX isoforms (1–6)...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/45/2/114 |
| _version_ | 1797621681969168384 |
|---|---|
| author | Fadime Aydın Köse Aysun Pabuccuoglu Miray Karakoyun Sema Aydogdu |
| author_facet | Fadime Aydın Köse Aysun Pabuccuoglu Miray Karakoyun Sema Aydogdu |
| author_sort | Fadime Aydın Köse |
| collection | DOAJ |
| description | Celiac disease (CD) is an autoimmune enteropathy. Peroxiredoxins (PRDXs) are powerful antioxidant enzymes having an important role in significant cellular pathways including cell survival, apoptosis, and inflammation. This study aimed at investigating the expression levels of all PRDX isoforms (1–6) and their possible relationships with a transcription factor, HIF-1α, in the small intestinal tissue samples of pediatric CD patients. The study groups consisted of first-diagnosed CD patients (<i>n</i> = 7) and non-CD patients with functional gastrointestinal tract disorders as the controls (<i>n</i> = 7). The PRDXs and HIF-1α expression levels were determined by using real-time PCR and Western blotting in duodenal biopsy samples. It was observed that the mRNA and protein expression levels of PRDX 5 were significantly higher in the CD patients, whereas the PRDX 1, -2, and -4 expressions were decreased in each case compared to the control group. No significant differences were detected in the PRDX 3 and PRDX 6 expressions. The expression of HIF-1α was also significantly elevated in CD patients. These findings indicate, for the first time, that PRDXs, particularly PRDX 5, may play a significant role in the pathogenesis of CD. Furthermore, our results suggest that HIF-1α may upregulate PRDX-5 transcription in the duodenal tissue of CD. |
| first_indexed | 2024-03-11T08:59:25Z |
| format | Article |
| id | doaj.art-e679e86d33994214b53d450807aa00a9 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T08:59:25Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-e679e86d33994214b53d450807aa00a92023-11-16T19:51:19ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-02-014521779179310.3390/cimb45020114Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease PatientsFadime Aydın Köse0Aysun Pabuccuoglu1Miray Karakoyun2Sema Aydogdu3Department of Biochemistry, Faculty of Pharmacy, Izmir Katip Celebi University, Izmir 35620, TurkeyDepartment of Biochemistry, Faculty of Pharmacy, Ege University, Izmir 35040, TurkeyDepartment of Child Health and Diseases, Faculty of Medicine, Ege University, Izmir 35040, TurkeyDepartment of Child Health and Diseases, Faculty of Medicine, Ege University, Izmir 35040, TurkeyCeliac disease (CD) is an autoimmune enteropathy. Peroxiredoxins (PRDXs) are powerful antioxidant enzymes having an important role in significant cellular pathways including cell survival, apoptosis, and inflammation. This study aimed at investigating the expression levels of all PRDX isoforms (1–6) and their possible relationships with a transcription factor, HIF-1α, in the small intestinal tissue samples of pediatric CD patients. The study groups consisted of first-diagnosed CD patients (<i>n</i> = 7) and non-CD patients with functional gastrointestinal tract disorders as the controls (<i>n</i> = 7). The PRDXs and HIF-1α expression levels were determined by using real-time PCR and Western blotting in duodenal biopsy samples. It was observed that the mRNA and protein expression levels of PRDX 5 were significantly higher in the CD patients, whereas the PRDX 1, -2, and -4 expressions were decreased in each case compared to the control group. No significant differences were detected in the PRDX 3 and PRDX 6 expressions. The expression of HIF-1α was also significantly elevated in CD patients. These findings indicate, for the first time, that PRDXs, particularly PRDX 5, may play a significant role in the pathogenesis of CD. Furthermore, our results suggest that HIF-1α may upregulate PRDX-5 transcription in the duodenal tissue of CD.https://www.mdpi.com/1467-3045/45/2/114celiac disease small intestineinflammationantioxidantperoxiredoxinhypoxia-inducible factor-1alpha |
| spellingShingle | Fadime Aydın Köse Aysun Pabuccuoglu Miray Karakoyun Sema Aydogdu Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients Current Issues in Molecular Biology celiac disease small intestine inflammation antioxidant peroxiredoxin hypoxia-inducible factor-1alpha |
| title | Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients |
| title_full | Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients |
| title_fullStr | Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients |
| title_full_unstemmed | Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients |
| title_short | Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients |
| title_sort | peroxiredoxins and hypoxia inducible factor 1α in duodenal tissue emerging factors in the pathophysiology of pediatric celiac disease patients |
| topic | celiac disease small intestine inflammation antioxidant peroxiredoxin hypoxia-inducible factor-1alpha |
| url | https://www.mdpi.com/1467-3045/45/2/114 |
| work_keys_str_mv | AT fadimeaydınkose peroxiredoxinsandhypoxiainduciblefactor1ainduodenaltissueemergingfactorsinthepathophysiologyofpediatricceliacdiseasepatients AT aysunpabuccuoglu peroxiredoxinsandhypoxiainduciblefactor1ainduodenaltissueemergingfactorsinthepathophysiologyofpediatricceliacdiseasepatients AT miraykarakoyun peroxiredoxinsandhypoxiainduciblefactor1ainduodenaltissueemergingfactorsinthepathophysiologyofpediatricceliacdiseasepatients AT semaaydogdu peroxiredoxinsandhypoxiainduciblefactor1ainduodenaltissueemergingfactorsinthepathophysiologyofpediatricceliacdiseasepatients |