Optimization of Ganoderma leucocontextum Fermented Tea Medium Formula Based on High-yield Extracellular Crude
To investigate the optimal nutritional conditions and their contributing factors for the secretion of extracellular crude polysaccharides with Ganoderma leucocontextum on a liquid tea substrate, this study optimized the nutritional conditions of its extracellular crude polysaccharides formation by o...
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| Format: | Article |
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The editorial department of Science and Technology of Food Industry
2024-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023040040 |
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| author | Mingjuan MAO Di LIU Huiming ZHOU Yuying BAI Peng HONG Jialin WANG Liubo YANG Zhenmin CHEN |
| author_facet | Mingjuan MAO Di LIU Huiming ZHOU Yuying BAI Peng HONG Jialin WANG Liubo YANG Zhenmin CHEN |
| author_sort | Mingjuan MAO |
| collection | DOAJ |
| description | To investigate the optimal nutritional conditions and their contributing factors for the secretion of extracellular crude polysaccharides with Ganoderma leucocontextum on a liquid tea substrate, this study optimized the nutritional conditions of its extracellular crude polysaccharides formation by orthogonal experiment and analysis of variance on the basis of the single-factor test, and explored the relevance between its liquid culture characteristics and the secretion of extracellular crude polysaccharides using correlation and path analyses. The results of study revealed that the greatest output of extracellular crude polysaccharide (1.49±0.33 g/L) was achieved on a tea culture medium of 50 g/L potato, 40 g/L glucose, 3 g/L laterite, 20 g/L Pu'er tea and 0.075 mm tea powder particle size, and was affected by each nutritional condition in the descending order of glucose>tea variety>laterite>potato>tea powder particle size. Moreover, the secretion of extracellular crude polysaccharides in G. leucocontextum was significantly impacted by the soluble solids of the fermentation broth and the number of mycelium balls, with the former having the greatest indirect effect while the latter, the greatest direct and combined effect on it. In conclusion, the nutritional conditions (variety, concentration, particle size, etc.) of the tea culture medium and the liquid culture characteristics of G. leucocontextum exerted a significant and interactive effect on the secretion of extracellular crude polysaccharides, and the aforesaid results may provide a theoretical reference for extraction of polysaccharides from this mushroom and development of it as functional foods. |
| first_indexed | 2024-03-08T08:11:59Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-08T08:11:59Z |
| publishDate | 2024-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-e67e7ee3b3f2469baf0ea5cb1e5c714c2024-02-02T08:40:58ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-02-014549310010.13386/j.issn1002-0306.20230400402023040040-4Optimization of Ganoderma leucocontextum Fermented Tea Medium Formula Based on High-yield Extracellular CrudeMingjuan MAO0Di LIU1Huiming ZHOU2Yuying BAI3Peng HONG4Jialin WANG5Liubo YANG6Zhenmin CHEN7School of Biotechnology and Engineering, West Yunnan University, Lincang 677000, ChinaAgricultural College, Yanbian University, Yanji 133002, ChinaSchool of Biotechnology and Engineering, West Yunnan University, Lincang 677000, ChinaSchool of Biotechnology and Engineering, West Yunnan University, Lincang 677000, ChinaSchool of Biotechnology and Engineering, West Yunnan University, Lincang 677000, ChinaSchool of Biotechnology and Engineering, West Yunnan University, Lincang 677000, ChinaSchool of Biotechnology and Engineering, West Yunnan University, Lincang 677000, ChinaSchool of Biotechnology and Engineering, West Yunnan University, Lincang 677000, ChinaTo investigate the optimal nutritional conditions and their contributing factors for the secretion of extracellular crude polysaccharides with Ganoderma leucocontextum on a liquid tea substrate, this study optimized the nutritional conditions of its extracellular crude polysaccharides formation by orthogonal experiment and analysis of variance on the basis of the single-factor test, and explored the relevance between its liquid culture characteristics and the secretion of extracellular crude polysaccharides using correlation and path analyses. The results of study revealed that the greatest output of extracellular crude polysaccharide (1.49±0.33 g/L) was achieved on a tea culture medium of 50 g/L potato, 40 g/L glucose, 3 g/L laterite, 20 g/L Pu'er tea and 0.075 mm tea powder particle size, and was affected by each nutritional condition in the descending order of glucose>tea variety>laterite>potato>tea powder particle size. Moreover, the secretion of extracellular crude polysaccharides in G. leucocontextum was significantly impacted by the soluble solids of the fermentation broth and the number of mycelium balls, with the former having the greatest indirect effect while the latter, the greatest direct and combined effect on it. In conclusion, the nutritional conditions (variety, concentration, particle size, etc.) of the tea culture medium and the liquid culture characteristics of G. leucocontextum exerted a significant and interactive effect on the secretion of extracellular crude polysaccharides, and the aforesaid results may provide a theoretical reference for extraction of polysaccharides from this mushroom and development of it as functional foods.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023040040ganoderma leucocontextumtea substrateliquid fermentationextracellular crude polysaccharidesoptimization experiment |
| spellingShingle | Mingjuan MAO Di LIU Huiming ZHOU Yuying BAI Peng HONG Jialin WANG Liubo YANG Zhenmin CHEN Optimization of Ganoderma leucocontextum Fermented Tea Medium Formula Based on High-yield Extracellular Crude Shipin gongye ke-ji ganoderma leucocontextum tea substrate liquid fermentation extracellular crude polysaccharides optimization experiment |
| title | Optimization of Ganoderma leucocontextum Fermented Tea Medium Formula Based on High-yield Extracellular Crude |
| title_full | Optimization of Ganoderma leucocontextum Fermented Tea Medium Formula Based on High-yield Extracellular Crude |
| title_fullStr | Optimization of Ganoderma leucocontextum Fermented Tea Medium Formula Based on High-yield Extracellular Crude |
| title_full_unstemmed | Optimization of Ganoderma leucocontextum Fermented Tea Medium Formula Based on High-yield Extracellular Crude |
| title_short | Optimization of Ganoderma leucocontextum Fermented Tea Medium Formula Based on High-yield Extracellular Crude |
| title_sort | optimization of ganoderma leucocontextum fermented tea medium formula based on high yield extracellular crude |
| topic | ganoderma leucocontextum tea substrate liquid fermentation extracellular crude polysaccharides optimization experiment |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023040040 |
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