Ultrasensitive detection of proteins and sugars at single-cell level
Each cell produces its own responses even if it appears identical to other cells. To analyze these individual cell characteristics, we need to measure trace amounts of molecules in a single cell. Nucleic acids in a single cell can be easily amplified by polymerase chain reaction, but single-cell mea...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2016-01-01
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| Series: | Communicative & Integrative Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19420889.2015.1124201 |
| _version_ | 1818855165673865216 |
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| author | Satoshi Watabe Mika Morikawa Mugiho Kaneda Kazunari Nakaishi Akira Nakatsuma Masaki Ninomiya Teruki Yoshimura Toshiaki Miura Etsuro Ito |
| author_facet | Satoshi Watabe Mika Morikawa Mugiho Kaneda Kazunari Nakaishi Akira Nakatsuma Masaki Ninomiya Teruki Yoshimura Toshiaki Miura Etsuro Ito |
| author_sort | Satoshi Watabe |
| collection | DOAJ |
| description | Each cell produces its own responses even if it appears identical to other cells. To analyze these individual cell characteristics, we need to measure trace amounts of molecules in a single cell. Nucleic acids in a single cell can be easily amplified by polymerase chain reaction, but single-cell measurement of proteins and sugars will require de novo techniques. In the present study, we outline the techniques we have developed toward this end. For proteins, our ultrasensitive enzyme-linked immunosorbent assay (ELISA) coupled with thionicotinamide-adenine dinucleotide cycling can detect proteins at subattomoles per assay. For sugars, fluorescence correlation spectroscopy coupled with glucose oxidase-catalyzed reaction allows us to measure glucose at tens of nM. Our methods thus offer versatile techniques for single-cell-level analyses, and they are hoped to strongly promote single-cell biology as well as to develop noninvasive tests in clinical medicine. |
| first_indexed | 2024-12-19T08:04:16Z |
| format | Article |
| id | doaj.art-e682f16208de4996961d62f55ac8170f |
| institution | Directory Open Access Journal |
| issn | 1942-0889 |
| language | English |
| last_indexed | 2024-12-19T08:04:16Z |
| publishDate | 2016-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Communicative & Integrative Biology |
| spelling | doaj.art-e682f16208de4996961d62f55ac8170f2022-12-21T20:29:47ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892016-01-019110.1080/19420889.2015.11242011124201Ultrasensitive detection of proteins and sugars at single-cell levelSatoshi Watabe0Mika Morikawa1Mugiho Kaneda2Kazunari Nakaishi3Akira Nakatsuma4Masaki Ninomiya5Teruki Yoshimura6Toshiaki Miura7Etsuro Ito8BL Co., Ltd.R&D Headquarters, TAUNS Laboratories, Inc.Kagawa School of Pharmaceutical Sciences, Tokushima Bunri UniversityR&D Headquarters, TAUNS Laboratories, Inc.Kagawa School of Pharmaceutical Sciences, Tokushima Bunri UniversityKagawa School of Pharmaceutical Sciences, Tokushima Bunri UniversityFaculty of Pharmaceutical Sciences, Health Sciences University of HokkaidoGraduate School of Pharmaceutical Sciences, Hokkaido UniversityKagawa School of Pharmaceutical Sciences, Tokushima Bunri UniversityEach cell produces its own responses even if it appears identical to other cells. To analyze these individual cell characteristics, we need to measure trace amounts of molecules in a single cell. Nucleic acids in a single cell can be easily amplified by polymerase chain reaction, but single-cell measurement of proteins and sugars will require de novo techniques. In the present study, we outline the techniques we have developed toward this end. For proteins, our ultrasensitive enzyme-linked immunosorbent assay (ELISA) coupled with thionicotinamide-adenine dinucleotide cycling can detect proteins at subattomoles per assay. For sugars, fluorescence correlation spectroscopy coupled with glucose oxidase-catalyzed reaction allows us to measure glucose at tens of nM. Our methods thus offer versatile techniques for single-cell-level analyses, and they are hoped to strongly promote single-cell biology as well as to develop noninvasive tests in clinical medicine.http://dx.doi.org/10.1080/19420889.2015.1124201adiponectinfluorescence correlation spectroscopyHIV-1 p24insulinthio-NAD cycling |
| spellingShingle | Satoshi Watabe Mika Morikawa Mugiho Kaneda Kazunari Nakaishi Akira Nakatsuma Masaki Ninomiya Teruki Yoshimura Toshiaki Miura Etsuro Ito Ultrasensitive detection of proteins and sugars at single-cell level Communicative & Integrative Biology adiponectin fluorescence correlation spectroscopy HIV-1 p24 insulin thio-NAD cycling |
| title | Ultrasensitive detection of proteins and sugars at single-cell level |
| title_full | Ultrasensitive detection of proteins and sugars at single-cell level |
| title_fullStr | Ultrasensitive detection of proteins and sugars at single-cell level |
| title_full_unstemmed | Ultrasensitive detection of proteins and sugars at single-cell level |
| title_short | Ultrasensitive detection of proteins and sugars at single-cell level |
| title_sort | ultrasensitive detection of proteins and sugars at single cell level |
| topic | adiponectin fluorescence correlation spectroscopy HIV-1 p24 insulin thio-NAD cycling |
| url | http://dx.doi.org/10.1080/19420889.2015.1124201 |
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