Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa
The influence of basal media composition, concentration of plant growth regulators (PGRs), and the developmental stage of primary explants (dormancy, stage of bud opening and fruit ripening) on the initiation phase of nine Magnolia genotypes, including M. stellata /Sieb. & Zucc./Maxim., M. × sou...
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| Format: | Article |
| Language: | English |
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Sciendo
2020-05-01
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| Series: | Folia Oecologica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/foecol-2020-0005 |
| _version_ | 1830187878605389824 |
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| author | Konôpková Jana Košútová Dominika Ferus Peter |
| author_facet | Konôpková Jana Košútová Dominika Ferus Peter |
| author_sort | Konôpková Jana |
| collection | DOAJ |
| description | The influence of basal media composition, concentration of plant growth regulators (PGRs), and the developmental stage of primary explants (dormancy, stage of bud opening and fruit ripening) on the initiation phase of nine Magnolia genotypes, including M. stellata /Sieb. & Zucc./Maxim., M. × soulangeana ‘Rustica Rubra’, M. denudata Desr., M. × soulangeana ‘Alexandrina’, M. liliiflora Desr., M. officinalis var. biloba Rehd. & Wils., M. salicifolia Maxim., M. × soulangeana ‘Lennei’, and M. kobus DC, was evaluated. The highest efficiency of primary culture initiation of seven Magnolia genotypes (except for M. liliiflora and M. salicifolia) was achieved from primary explants collected in the bud opening stage. A high positive correlation was found between total tannins and efficiency of the primary culture initiation at the fruit ripening stage (r = 0.833). Standardi and Catalano medium (S2) with 0.5 mg l−1 of 6-benzylaminopurine (BAP) was the most appropriate for multiplication of M. × soulangeana ‘Alexandrina’, whereas tissue cultures of M. × soulangeana ‘Lennei’ proliferated and grew better on S2 medium with 1.0 mg l−1 of BAP and 1.0 g l−1 of polyvinylpyrrolidone. The requirements for the composition of basal media and concentration of PGRs in the initiation and multiplication stages of micropropagation of various Magnolia species and cultivars are genotype-specific. |
| first_indexed | 2024-12-17T22:35:45Z |
| format | Article |
| id | doaj.art-e68435ccb20e4252a57f7986e3ff1224 |
| institution | Directory Open Access Journal |
| issn | 1338-7014 |
| language | English |
| last_indexed | 2024-12-17T22:35:45Z |
| publishDate | 2020-05-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Folia Oecologica |
| spelling | doaj.art-e68435ccb20e4252a57f7986e3ff12242022-12-21T21:30:06ZengSciendoFolia Oecologica1338-70142020-05-01471344410.2478/foecol-2020-0005foecol-2020-0005Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxaKonôpková Jana0Košútová Dominika1Ferus Peter2Mlyňany Arboretum, Institute of Forest Ecology, Slovak Academy of Sciences, Vieska nad Žitavou 178, SK-95152 Slepčany, SlovakiaMlyňany Arboretum, Institute of Forest Ecology, Slovak Academy of Sciences, Vieska nad Žitavou 178, SK-95152 Slepčany, SlovakiaMlyňany Arboretum, Institute of Forest Ecology, Slovak Academy of Sciences, Vieska nad Žitavou 178, SK-95152 Slepčany, SlovakiaThe influence of basal media composition, concentration of plant growth regulators (PGRs), and the developmental stage of primary explants (dormancy, stage of bud opening and fruit ripening) on the initiation phase of nine Magnolia genotypes, including M. stellata /Sieb. & Zucc./Maxim., M. × soulangeana ‘Rustica Rubra’, M. denudata Desr., M. × soulangeana ‘Alexandrina’, M. liliiflora Desr., M. officinalis var. biloba Rehd. & Wils., M. salicifolia Maxim., M. × soulangeana ‘Lennei’, and M. kobus DC, was evaluated. The highest efficiency of primary culture initiation of seven Magnolia genotypes (except for M. liliiflora and M. salicifolia) was achieved from primary explants collected in the bud opening stage. A high positive correlation was found between total tannins and efficiency of the primary culture initiation at the fruit ripening stage (r = 0.833). Standardi and Catalano medium (S2) with 0.5 mg l−1 of 6-benzylaminopurine (BAP) was the most appropriate for multiplication of M. × soulangeana ‘Alexandrina’, whereas tissue cultures of M. × soulangeana ‘Lennei’ proliferated and grew better on S2 medium with 1.0 mg l−1 of BAP and 1.0 g l−1 of polyvinylpyrrolidone. The requirements for the composition of basal media and concentration of PGRs in the initiation and multiplication stages of micropropagation of various Magnolia species and cultivars are genotype-specific.https://doi.org/10.2478/foecol-2020-0005in vitro propagationmagnoliamultiplicationprimary culture initiation |
| spellingShingle | Konôpková Jana Košútová Dominika Ferus Peter Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa Folia Oecologica in vitro propagation magnolia multiplication primary culture initiation |
| title | Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa |
| title_full | Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa |
| title_fullStr | Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa |
| title_full_unstemmed | Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa |
| title_short | Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa |
| title_sort | genotype specific requirements for in vitro culture initiation and multiplication of magnolia taxa |
| topic | in vitro propagation magnolia multiplication primary culture initiation |
| url | https://doi.org/10.2478/foecol-2020-0005 |
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