Excitatory neurotransmission activates compartmentalized calcium transients in Müller glia without affecting lateral process motility
Neural activity has been implicated in the motility and outgrowth of glial cell processes throughout the central nervous system. Here, we explore this phenomenon in Müller glia, which are specialized radial astroglia that are the predominant glial type of the vertebrate retina. Müller glia extend fi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-12-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/73202 |
| _version_ | 1797998475192827904 |
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| author | Joshua M Tworig Chandler J Coate Marla B Feller |
| author_facet | Joshua M Tworig Chandler J Coate Marla B Feller |
| author_sort | Joshua M Tworig |
| collection | DOAJ |
| description | Neural activity has been implicated in the motility and outgrowth of glial cell processes throughout the central nervous system. Here, we explore this phenomenon in Müller glia, which are specialized radial astroglia that are the predominant glial type of the vertebrate retina. Müller glia extend fine filopodia-like processes into retinal synaptic layers, in similar fashion to brain astrocytes and radial glia that exhibit perisynaptic processes. Using two-photon volumetric imaging, we found that during the second postnatal week, Müller glial processes were highly dynamic, with rapid extensions and retractions that were mediated by cytoskeletal rearrangements. During this same stage of development, retinal waves led to increases in cytosolic calcium within Müller glial lateral processes and stalks. These regions comprised distinct calcium compartments, distinguished by variable participation in waves, timing, and sensitivity to an M1 muscarinic acetylcholine receptor antagonist. However, we found that motility of lateral processes was unaffected by the presence of pharmacological agents that enhanced or blocked wave-associated calcium transients. Finally, we found that mice lacking normal cholinergic waves in the first postnatal week also exhibited normal Müller glial process morphology. Hence, outgrowth of Müller glial lateral processes into synaptic layers is determined by factors that are independent of neuronal activity. |
| first_indexed | 2024-04-11T10:49:20Z |
| format | Article |
| id | doaj.art-e688afd306b94c618d29e5f39cfc96e2 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:49:20Z |
| publishDate | 2021-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e688afd306b94c618d29e5f39cfc96e22022-12-22T04:28:58ZengeLife Sciences Publications LtdeLife2050-084X2021-12-011010.7554/eLife.73202Excitatory neurotransmission activates compartmentalized calcium transients in Müller glia without affecting lateral process motilityJoshua M Tworig0https://orcid.org/0000-0001-7798-4480Chandler J Coate1Marla B Feller2https://orcid.org/0000-0002-9137-5849Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesNeural activity has been implicated in the motility and outgrowth of glial cell processes throughout the central nervous system. Here, we explore this phenomenon in Müller glia, which are specialized radial astroglia that are the predominant glial type of the vertebrate retina. Müller glia extend fine filopodia-like processes into retinal synaptic layers, in similar fashion to brain astrocytes and radial glia that exhibit perisynaptic processes. Using two-photon volumetric imaging, we found that during the second postnatal week, Müller glial processes were highly dynamic, with rapid extensions and retractions that were mediated by cytoskeletal rearrangements. During this same stage of development, retinal waves led to increases in cytosolic calcium within Müller glial lateral processes and stalks. These regions comprised distinct calcium compartments, distinguished by variable participation in waves, timing, and sensitivity to an M1 muscarinic acetylcholine receptor antagonist. However, we found that motility of lateral processes was unaffected by the presence of pharmacological agents that enhanced or blocked wave-associated calcium transients. Finally, we found that mice lacking normal cholinergic waves in the first postnatal week also exhibited normal Müller glial process morphology. Hence, outgrowth of Müller glial lateral processes into synaptic layers is determined by factors that are independent of neuronal activity.https://elifesciences.org/articles/73202retina developmentneuron-glia interactionstwo-photon calcium imagingglial morphologyspontaneous activity |
| spellingShingle | Joshua M Tworig Chandler J Coate Marla B Feller Excitatory neurotransmission activates compartmentalized calcium transients in Müller glia without affecting lateral process motility eLife retina development neuron-glia interactions two-photon calcium imaging glial morphology spontaneous activity |
| title | Excitatory neurotransmission activates compartmentalized calcium transients in Müller glia without affecting lateral process motility |
| title_full | Excitatory neurotransmission activates compartmentalized calcium transients in Müller glia without affecting lateral process motility |
| title_fullStr | Excitatory neurotransmission activates compartmentalized calcium transients in Müller glia without affecting lateral process motility |
| title_full_unstemmed | Excitatory neurotransmission activates compartmentalized calcium transients in Müller glia without affecting lateral process motility |
| title_short | Excitatory neurotransmission activates compartmentalized calcium transients in Müller glia without affecting lateral process motility |
| title_sort | excitatory neurotransmission activates compartmentalized calcium transients in muller glia without affecting lateral process motility |
| topic | retina development neuron-glia interactions two-photon calcium imaging glial morphology spontaneous activity |
| url | https://elifesciences.org/articles/73202 |
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