Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla
Abstract High-performance liquid chromatography–mass spectrometry (LC–MS) was used for comprehensive metabolomic fingerprinting of vanilla fruits prepared from the curing process. In this study, the metabolic changes of vanilla pods and vanilla beans were characterized using MS-based metabolomics to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-06-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0413-2 |
| _version_ | 1818324722413207552 |
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| author | Fenglin Gu Yonggan Chen Yinghua Hong Yiming Fang Lehe Tan |
| author_facet | Fenglin Gu Yonggan Chen Yinghua Hong Yiming Fang Lehe Tan |
| author_sort | Fenglin Gu |
| collection | DOAJ |
| description | Abstract High-performance liquid chromatography–mass spectrometry (LC–MS) was used for comprehensive metabolomic fingerprinting of vanilla fruits prepared from the curing process. In this study, the metabolic changes of vanilla pods and vanilla beans were characterized using MS-based metabolomics to elucidate the biosynthesis of vanillin. The vanilla pods were significantly different from vanilla beans. Seven pathways of vanillin biosynthesis were constructed, namely, glucovanillin, glucose, cresol, capsaicin, vanillyl alcohol, tyrosine, and phenylalanine pathways. Investigations demonstrated that glucose, cresol, capsaicin, and vanillyl alcohol pathway were detected in a wide range of distribution in microbial metabolism. Thus, microorganisms might have participated in vanillin biosynthesis during vanilla curing. Furthermore, the ion strength of glucovanillin was stable, which indicated that glucovanillin only participated in the vanillin biosynthesis during the curing of vanilla. |
| first_indexed | 2024-12-13T11:33:06Z |
| format | Article |
| id | doaj.art-e6890205f52e4dfc9eeb53866fdefe0f |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-13T11:33:06Z |
| publishDate | 2017-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-e6890205f52e4dfc9eeb53866fdefe0f2022-12-21T23:47:53ZengSpringerOpenAMB Express2191-08552017-06-01711910.1186/s13568-017-0413-2Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanillaFenglin Gu0Yonggan Chen1Yinghua Hong2Yiming Fang3Lehe Tan4Spice and Beverage Research Institute, CATASCollege of Tropical Biology and Agronomy, Hainan Tropical Ocean UniversitySpice and Beverage Research Institute, CATASSpice and Beverage Research Institute, CATASSpice and Beverage Research Institute, CATASAbstract High-performance liquid chromatography–mass spectrometry (LC–MS) was used for comprehensive metabolomic fingerprinting of vanilla fruits prepared from the curing process. In this study, the metabolic changes of vanilla pods and vanilla beans were characterized using MS-based metabolomics to elucidate the biosynthesis of vanillin. The vanilla pods were significantly different from vanilla beans. Seven pathways of vanillin biosynthesis were constructed, namely, glucovanillin, glucose, cresol, capsaicin, vanillyl alcohol, tyrosine, and phenylalanine pathways. Investigations demonstrated that glucose, cresol, capsaicin, and vanillyl alcohol pathway were detected in a wide range of distribution in microbial metabolism. Thus, microorganisms might have participated in vanillin biosynthesis during vanilla curing. Furthermore, the ion strength of glucovanillin was stable, which indicated that glucovanillin only participated in the vanillin biosynthesis during the curing of vanilla.http://link.springer.com/article/10.1186/s13568-017-0413-2Vanilla curingLC–MSMetabolomicsVanillin biosynthesis |
| spellingShingle | Fenglin Gu Yonggan Chen Yinghua Hong Yiming Fang Lehe Tan Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla AMB Express Vanilla curing LC–MS Metabolomics Vanillin biosynthesis |
| title | Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla |
| title_full | Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla |
| title_fullStr | Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla |
| title_full_unstemmed | Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla |
| title_short | Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla |
| title_sort | comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla |
| topic | Vanilla curing LC–MS Metabolomics Vanillin biosynthesis |
| url | http://link.springer.com/article/10.1186/s13568-017-0413-2 |
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