Spiroplasma infection in Harmonia axyridis - Diversity and multiple infection.
The heritable endosymbiotic bacterium Spiroplasma is found in the harlequin ladybird Harmonia axyridis. The proportion of beetles infected with Spiroplasma in different native H. axyridis populations varies from 2% to 49%. We investigated the polymorphism of Spiroplasma strains in samples from indiv...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5973594?pdf=render |
| _version_ | 1811287653009588224 |
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| author | Irina Goryacheva Alla Blekhman Boris Andrianov Denis Romanov Ilya Zakharov |
| author_facet | Irina Goryacheva Alla Blekhman Boris Andrianov Denis Romanov Ilya Zakharov |
| author_sort | Irina Goryacheva |
| collection | DOAJ |
| description | The heritable endosymbiotic bacterium Spiroplasma is found in the harlequin ladybird Harmonia axyridis. The proportion of beetles infected with Spiroplasma in different native H. axyridis populations varies from 2% to 49%. We investigated the polymorphism of Spiroplasma strains in samples from individual beetles from Kyoto, Vladivostok, Troitsa Bay, Novosibirsk, and Gorno-Altaisk. To identify Spiroplasma strains, we analyzed nucleotide polymorphisms of the 16S rRNA gene and the ribosomal internal transcribed spacer (ITS1). The majority of infected beetles were infected with two or more Spiroplasma strains. We measured Spiroplasma density in beetles with different infection status using quantitative PCR. The abundance of Spiroplasma in samples with a single infection is an order of magnitude lower than in samples with multiple infections. Density dependent biological effects of Spiroplasma are discussed. |
| first_indexed | 2024-04-13T03:22:21Z |
| format | Article |
| id | doaj.art-e68ae7e6085b4d29ba00878e9bb58d3b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T03:22:21Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e68ae7e6085b4d29ba00878e9bb58d3b2022-12-22T03:04:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01135e019819010.1371/journal.pone.0198190Spiroplasma infection in Harmonia axyridis - Diversity and multiple infection.Irina GoryachevaAlla BlekhmanBoris AndrianovDenis RomanovIlya ZakharovThe heritable endosymbiotic bacterium Spiroplasma is found in the harlequin ladybird Harmonia axyridis. The proportion of beetles infected with Spiroplasma in different native H. axyridis populations varies from 2% to 49%. We investigated the polymorphism of Spiroplasma strains in samples from individual beetles from Kyoto, Vladivostok, Troitsa Bay, Novosibirsk, and Gorno-Altaisk. To identify Spiroplasma strains, we analyzed nucleotide polymorphisms of the 16S rRNA gene and the ribosomal internal transcribed spacer (ITS1). The majority of infected beetles were infected with two or more Spiroplasma strains. We measured Spiroplasma density in beetles with different infection status using quantitative PCR. The abundance of Spiroplasma in samples with a single infection is an order of magnitude lower than in samples with multiple infections. Density dependent biological effects of Spiroplasma are discussed.http://europepmc.org/articles/PMC5973594?pdf=render |
| spellingShingle | Irina Goryacheva Alla Blekhman Boris Andrianov Denis Romanov Ilya Zakharov Spiroplasma infection in Harmonia axyridis - Diversity and multiple infection. PLoS ONE |
| title | Spiroplasma infection in Harmonia axyridis - Diversity and multiple infection. |
| title_full | Spiroplasma infection in Harmonia axyridis - Diversity and multiple infection. |
| title_fullStr | Spiroplasma infection in Harmonia axyridis - Diversity and multiple infection. |
| title_full_unstemmed | Spiroplasma infection in Harmonia axyridis - Diversity and multiple infection. |
| title_short | Spiroplasma infection in Harmonia axyridis - Diversity and multiple infection. |
| title_sort | spiroplasma infection in harmonia axyridis diversity and multiple infection |
| url | http://europepmc.org/articles/PMC5973594?pdf=render |
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