A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions
Bioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor ce...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/3/1/10 |
| _version_ | 1818907219171737600 |
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| author | Jinsoo Yoon Christopher R. Parish Lucy A. Coupland |
| author_facet | Jinsoo Yoon Christopher R. Parish Lucy A. Coupland |
| author_sort | Jinsoo Yoon |
| collection | DOAJ |
| description | Bioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor cells with different stromal cell types for analysis of the effects of intercellular cross-talk on tumor cell migration. We describe a novel migration assay using bioluminescent tumor cell lines that is rapid, accurate, and permits the study of the effects of tumor cell-stromal cell interactions on tumor cell migratory behavior. |
| first_indexed | 2024-12-19T21:51:38Z |
| format | Article |
| id | doaj.art-e68dcbe5d17d46eaadf537793d41cc3c |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-12-19T21:51:38Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-e68dcbe5d17d46eaadf537793d41cc3c2022-12-21T20:04:24ZengMDPI AGMethods and Protocols2409-92792020-01-01311010.3390/mps3010010mps3010010A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell InteractionsJinsoo Yoon0Christopher R. Parish1Lucy A. Coupland2The ACRF Department of Cancer Biology & Therapeutics, John Curtin School of Medical Research, The Australian National University, Canberra 2601, AustraliaThe ACRF Department of Cancer Biology & Therapeutics, John Curtin School of Medical Research, The Australian National University, Canberra 2601, AustraliaThe ACRF Department of Cancer Biology & Therapeutics, John Curtin School of Medical Research, The Australian National University, Canberra 2601, AustraliaBioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor cells with different stromal cell types for analysis of the effects of intercellular cross-talk on tumor cell migration. We describe a novel migration assay using bioluminescent tumor cell lines that is rapid, accurate, and permits the study of the effects of tumor cell-stromal cell interactions on tumor cell migratory behavior.https://www.mdpi.com/2409-9279/3/1/10transwell migration assaytumor/stromal interactionsbioluminescence |
| spellingShingle | Jinsoo Yoon Christopher R. Parish Lucy A. Coupland A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions Methods and Protocols transwell migration assay tumor/stromal interactions bioluminescence |
| title | A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions |
| title_full | A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions |
| title_fullStr | A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions |
| title_full_unstemmed | A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions |
| title_short | A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions |
| title_sort | rapid and accurate bioluminescence based migration assay permitting analysis of tumor cell stromal cell interactions |
| topic | transwell migration assay tumor/stromal interactions bioluminescence |
| url | https://www.mdpi.com/2409-9279/3/1/10 |
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