Characterization and In Vitro evaluation of a novel coated nanocomposite porous 3D scaffold for bone repair
The aim of this study is to tissue engineer a 3D scaffold that can be used for load bearing segmental bone defects (SBDs) repair. Three different scaffolds were fabricated using cockle shell-derived CaCO<sub>3</sub> aragonite nanoparticles (CCAN), gelatin, dextran and dextrin with coated...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2019-03-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://vetmedmosul.com/article_159517_78c90de2246b07f55b929209aee8a165.pdf |
| _version_ | 1818575226821148672 |
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| author | Sahar Ibrahim Saffanah Mahmood Intan Shameha Binti Abdul Razak Loqman Mohamed Yusof Zaid Khudhur Mahmood Fufa I. Gimba Md Zuki Abu Bakar Zakaria |
| author_facet | Sahar Ibrahim Saffanah Mahmood Intan Shameha Binti Abdul Razak Loqman Mohamed Yusof Zaid Khudhur Mahmood Fufa I. Gimba Md Zuki Abu Bakar Zakaria |
| author_sort | Sahar Ibrahim |
| collection | DOAJ |
| description | The aim of this study is to tissue engineer a 3D scaffold that can be used for load bearing segmental bone defects (SBDs) repair. Three different scaffolds were fabricated using cockle shell-derived CaCO<sub>3</sub> aragonite nanoparticles (CCAN), gelatin, dextran and dextrin with coated framework via Freeze-Drying Method (FDM) labeled as 5211, 5211<sub>GTA+Alginate</sub>, 5211<sub>PLA</sub>. Scaffolds were assessed using Scanning Electron Microscopy (SEM). The cytocompatibility of the organized scaffolds was assessed using cells multiplication and alkaline phosphatase (ALP) concentration via <em>In Vitro</em> cell culture using human Fetal OsteoBlast cells line (hFOB). The results showed a substantial difference in ALP concentrations between the cultures of different scaffolds leachable medium during the study period. The biological evaluation also showed that three scaffolds did enhanced the osteoblast proliferation rate and improved the osteoblast function as demonstrated by the significant increase in ALP concentration. Engineering analyses showed that scaffolds possessed 3D interconnected homogenous porous structure with a porosity ranging 6%-49%, pore sizes ranging 8-345 µm, mechanical strength ranging 20-65 MPa, young’s modulus ranging 166-296 MPa and enzymatic degradation rate between 16%-38% within 2-10 weeks. The <em>in vitro</em> evaluation revealed that the scaffold 5211, 5211<sub>GTA+Alginate </sub>and 5211<sub>PLA</sub> fulfill all the main requirements to be considered as an ideal bone replacement. |
| first_indexed | 2024-12-15T00:37:22Z |
| format | Article |
| id | doaj.art-e68edb671109407aa96525866c4e52fb |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-12-15T00:37:22Z |
| publishDate | 2019-03-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-e68edb671109407aa96525866c4e52fb2022-12-21T22:41:46ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552019-03-0133115717310.33899/ijvs.2019.125548.1068159517Characterization and In Vitro evaluation of a novel coated nanocomposite porous 3D scaffold for bone repairSahar Ibrahim0Saffanah Mahmood1Intan Shameha Binti Abdul Razak2Loqman Mohamed Yusof3Zaid Khudhur Mahmood4Fufa I. Gimba5Md Zuki Abu Bakar Zakaria6Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Anatomy, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400,Serdang, Selangor Darul Ehsan, Malaysia.Department of Companion Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor Darul Ehsan, Malaysia.Department of Veterinary clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400,Serdang, Selangor Darul Ehsan, Malaysia.Avian influenza control project, Animal components, Taraba state ministry of Agriculture and Natural resources Jalingo, Taraba, Nigeria.Laboratory of Molecular Biomedicine, Institute of Biosciences, Universiti Putra Malaysia (UPM), 43400,Serdang, Selangor Darul Ehsan, MalaysiaThe aim of this study is to tissue engineer a 3D scaffold that can be used for load bearing segmental bone defects (SBDs) repair. Three different scaffolds were fabricated using cockle shell-derived CaCO<sub>3</sub> aragonite nanoparticles (CCAN), gelatin, dextran and dextrin with coated framework via Freeze-Drying Method (FDM) labeled as 5211, 5211<sub>GTA+Alginate</sub>, 5211<sub>PLA</sub>. Scaffolds were assessed using Scanning Electron Microscopy (SEM). The cytocompatibility of the organized scaffolds was assessed using cells multiplication and alkaline phosphatase (ALP) concentration via <em>In Vitro</em> cell culture using human Fetal OsteoBlast cells line (hFOB). The results showed a substantial difference in ALP concentrations between the cultures of different scaffolds leachable medium during the study period. The biological evaluation also showed that three scaffolds did enhanced the osteoblast proliferation rate and improved the osteoblast function as demonstrated by the significant increase in ALP concentration. Engineering analyses showed that scaffolds possessed 3D interconnected homogenous porous structure with a porosity ranging 6%-49%, pore sizes ranging 8-345 µm, mechanical strength ranging 20-65 MPa, young’s modulus ranging 166-296 MPa and enzymatic degradation rate between 16%-38% within 2-10 weeks. The <em>in vitro</em> evaluation revealed that the scaffold 5211, 5211<sub>GTA+Alginate </sub>and 5211<sub>PLA</sub> fulfill all the main requirements to be considered as an ideal bone replacement.https://vetmedmosul.com/article_159517_78c90de2246b07f55b929209aee8a165.pdfaragonitebionanocomposite3d porous scaffoldhfobbone repair |
| spellingShingle | Sahar Ibrahim Saffanah Mahmood Intan Shameha Binti Abdul Razak Loqman Mohamed Yusof Zaid Khudhur Mahmood Fufa I. Gimba Md Zuki Abu Bakar Zakaria Characterization and In Vitro evaluation of a novel coated nanocomposite porous 3D scaffold for bone repair Iraqi Journal of Veterinary Sciences aragonite bionanocomposite 3d porous scaffold hfob bone repair |
| title | Characterization and In Vitro evaluation of a novel coated nanocomposite porous 3D scaffold for bone repair |
| title_full | Characterization and In Vitro evaluation of a novel coated nanocomposite porous 3D scaffold for bone repair |
| title_fullStr | Characterization and In Vitro evaluation of a novel coated nanocomposite porous 3D scaffold for bone repair |
| title_full_unstemmed | Characterization and In Vitro evaluation of a novel coated nanocomposite porous 3D scaffold for bone repair |
| title_short | Characterization and In Vitro evaluation of a novel coated nanocomposite porous 3D scaffold for bone repair |
| title_sort | characterization and in vitro evaluation of a novel coated nanocomposite porous 3d scaffold for bone repair |
| topic | aragonite bionanocomposite 3d porous scaffold hfob bone repair |
| url | https://vetmedmosul.com/article_159517_78c90de2246b07f55b929209aee8a165.pdf |
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