Hepatoprotective Effect of the Penthorum Chinense Pursh Extract against the CCl<sub>4</sub>-Induced Acute Liver Injury via NF-κB and p38-MAPK PATHWAYS in Dogs
Acute liver injury (ALI), manifested by acute hepatocellular damages and necrosis, is a life-threatening clinical syndrome and Penthorum Chinense Pursh (PCP) is a well-known folk medicine practiced for liver-related diseases. This study aimed to investigate the ameliorative effects of PCP extract (P...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/12/5/569 |
| _version_ | 1797475883014946816 |
|---|---|
| author | Weilai Tao Xin Yue Ruiling Ye Fazul Nabi Yangfei Shang Zhaorong Zhu Bhutto Zohaib Ahmed Juan Liu |
| author_facet | Weilai Tao Xin Yue Ruiling Ye Fazul Nabi Yangfei Shang Zhaorong Zhu Bhutto Zohaib Ahmed Juan Liu |
| author_sort | Weilai Tao |
| collection | DOAJ |
| description | Acute liver injury (ALI), manifested by acute hepatocellular damages and necrosis, is a life-threatening clinical syndrome and Penthorum Chinense Pursh (PCP) is a well-known folk medicine practiced for liver-related diseases. This study aimed to investigate the ameliorative effects of PCP extract (PCPE) on carbon tetrachloride (CCl<sub>4</sub>) induced ALI in dogs via mitogen-activated protein kinase (MAPK) and Nuclear factor κB (NF-κB) signaling pathway. Healthy dogs were induced by CCl<sub>4</sub> and treated with different dosage regimes of PCPE for 7 days. CCl<sub>4</sub> produced acute liver injury and induced both oxidative stress and an inflammatory response in dogs. The PCPE significantly ameliorated and improved vacuolar inflammatory lesions in liver tissues during ALI, enhanced activity of superoxide dismutase, and restored glutathione peroxidase, further significantly reducing the indices of malondialdehyde and nitric oxide in serum. Inflammatory factors (IL-1β, IL-6, and TNF-α) were declined and anti-inflammatory factors (IL-10) were increased by the application of PCPE. PCPE treatment, down-regulated the MEKK4, MKK3, p38MAPK, MSK1, and NF-κB, and upregulated the IkB mRNA levels (<i>p</i> < 0.01) in ALI affected dogs. In conclusion, PCPE repaired acute liver injury by improving antioxidant enzymes and by reducing oxidation products. Furthermore, the PCPE inhibited the MAPK/NF-κB signaling pathway, which resulted in anti-inflammatory and antioxidant effects on ALI-induced dogs. In the future, PCPE could be a useful ethnomedicine in veterinary clinical practices for the treatment of liver injuries or failures. |
| first_indexed | 2024-03-09T20:50:01Z |
| format | Article |
| id | doaj.art-e68fe97228ed4c9386887f02e2e67975 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T20:50:01Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-e68fe97228ed4c9386887f02e2e679752023-11-23T22:36:33ZengMDPI AGAnimals2076-26152022-02-0112556910.3390/ani12050569Hepatoprotective Effect of the Penthorum Chinense Pursh Extract against the CCl<sub>4</sub>-Induced Acute Liver Injury via NF-κB and p38-MAPK PATHWAYS in DogsWeilai Tao0Xin Yue1Ruiling Ye2Fazul Nabi3Yangfei Shang4Zhaorong Zhu5Bhutto Zohaib Ahmed6Juan Liu7College of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaFaculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water, and Marine Sciences, Uthal 90150, PakistanCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaAcute liver injury (ALI), manifested by acute hepatocellular damages and necrosis, is a life-threatening clinical syndrome and Penthorum Chinense Pursh (PCP) is a well-known folk medicine practiced for liver-related diseases. This study aimed to investigate the ameliorative effects of PCP extract (PCPE) on carbon tetrachloride (CCl<sub>4</sub>) induced ALI in dogs via mitogen-activated protein kinase (MAPK) and Nuclear factor κB (NF-κB) signaling pathway. Healthy dogs were induced by CCl<sub>4</sub> and treated with different dosage regimes of PCPE for 7 days. CCl<sub>4</sub> produced acute liver injury and induced both oxidative stress and an inflammatory response in dogs. The PCPE significantly ameliorated and improved vacuolar inflammatory lesions in liver tissues during ALI, enhanced activity of superoxide dismutase, and restored glutathione peroxidase, further significantly reducing the indices of malondialdehyde and nitric oxide in serum. Inflammatory factors (IL-1β, IL-6, and TNF-α) were declined and anti-inflammatory factors (IL-10) were increased by the application of PCPE. PCPE treatment, down-regulated the MEKK4, MKK3, p38MAPK, MSK1, and NF-κB, and upregulated the IkB mRNA levels (<i>p</i> < 0.01) in ALI affected dogs. In conclusion, PCPE repaired acute liver injury by improving antioxidant enzymes and by reducing oxidation products. Furthermore, the PCPE inhibited the MAPK/NF-κB signaling pathway, which resulted in anti-inflammatory and antioxidant effects on ALI-induced dogs. In the future, PCPE could be a useful ethnomedicine in veterinary clinical practices for the treatment of liver injuries or failures.https://www.mdpi.com/2076-2615/12/5/569acute liver injuryPenthorum Chinense PurshMAPK/NF-κB signalingoxidative stressinflammation |
| spellingShingle | Weilai Tao Xin Yue Ruiling Ye Fazul Nabi Yangfei Shang Zhaorong Zhu Bhutto Zohaib Ahmed Juan Liu Hepatoprotective Effect of the Penthorum Chinense Pursh Extract against the CCl<sub>4</sub>-Induced Acute Liver Injury via NF-κB and p38-MAPK PATHWAYS in Dogs Animals acute liver injury Penthorum Chinense Pursh MAPK/NF-κB signaling oxidative stress inflammation |
| title | Hepatoprotective Effect of the Penthorum Chinense Pursh Extract against the CCl<sub>4</sub>-Induced Acute Liver Injury via NF-κB and p38-MAPK PATHWAYS in Dogs |
| title_full | Hepatoprotective Effect of the Penthorum Chinense Pursh Extract against the CCl<sub>4</sub>-Induced Acute Liver Injury via NF-κB and p38-MAPK PATHWAYS in Dogs |
| title_fullStr | Hepatoprotective Effect of the Penthorum Chinense Pursh Extract against the CCl<sub>4</sub>-Induced Acute Liver Injury via NF-κB and p38-MAPK PATHWAYS in Dogs |
| title_full_unstemmed | Hepatoprotective Effect of the Penthorum Chinense Pursh Extract against the CCl<sub>4</sub>-Induced Acute Liver Injury via NF-κB and p38-MAPK PATHWAYS in Dogs |
| title_short | Hepatoprotective Effect of the Penthorum Chinense Pursh Extract against the CCl<sub>4</sub>-Induced Acute Liver Injury via NF-κB and p38-MAPK PATHWAYS in Dogs |
| title_sort | hepatoprotective effect of the penthorum chinense pursh extract against the ccl sub 4 sub induced acute liver injury via nf κb and p38 mapk pathways in dogs |
| topic | acute liver injury Penthorum Chinense Pursh MAPK/NF-κB signaling oxidative stress inflammation |
| url | https://www.mdpi.com/2076-2615/12/5/569 |
| work_keys_str_mv | AT weilaitao hepatoprotectiveeffectofthepenthorumchinensepurshextractagainstthecclsub4subinducedacuteliverinjuryvianfkbandp38mapkpathwaysindogs AT xinyue hepatoprotectiveeffectofthepenthorumchinensepurshextractagainstthecclsub4subinducedacuteliverinjuryvianfkbandp38mapkpathwaysindogs AT ruilingye hepatoprotectiveeffectofthepenthorumchinensepurshextractagainstthecclsub4subinducedacuteliverinjuryvianfkbandp38mapkpathwaysindogs AT fazulnabi hepatoprotectiveeffectofthepenthorumchinensepurshextractagainstthecclsub4subinducedacuteliverinjuryvianfkbandp38mapkpathwaysindogs AT yangfeishang hepatoprotectiveeffectofthepenthorumchinensepurshextractagainstthecclsub4subinducedacuteliverinjuryvianfkbandp38mapkpathwaysindogs AT zhaorongzhu hepatoprotectiveeffectofthepenthorumchinensepurshextractagainstthecclsub4subinducedacuteliverinjuryvianfkbandp38mapkpathwaysindogs AT bhuttozohaibahmed hepatoprotectiveeffectofthepenthorumchinensepurshextractagainstthecclsub4subinducedacuteliverinjuryvianfkbandp38mapkpathwaysindogs AT juanliu hepatoprotectiveeffectofthepenthorumchinensepurshextractagainstthecclsub4subinducedacuteliverinjuryvianfkbandp38mapkpathwaysindogs |