Responses of the Macroalga <i>Ulva prolifera</i> Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics Approaches
Ocean acidification (OA) is a dramatic perturbation of seawater environments due to increasing anthropogenic emissions of CO<sub>2</sub>. Several studies indicated that OA frequently induces marine biota stress and a reduction of biodiversity. Here, we adopted the macroalga <i>Ulva...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/12/743 |
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| author | Carlos Sanchez-Arcos Debora Paris Valerio Mazzella Mirko Mutalipassi Maria Costantini Maria Cristina Buia Eric von Elert Adele Cutignano Valerio Zupo |
| author_facet | Carlos Sanchez-Arcos Debora Paris Valerio Mazzella Mirko Mutalipassi Maria Costantini Maria Cristina Buia Eric von Elert Adele Cutignano Valerio Zupo |
| author_sort | Carlos Sanchez-Arcos |
| collection | DOAJ |
| description | Ocean acidification (OA) is a dramatic perturbation of seawater environments due to increasing anthropogenic emissions of CO<sub>2</sub>. Several studies indicated that OA frequently induces marine biota stress and a reduction of biodiversity. Here, we adopted the macroalga <i>Ulva prolifera</i> as a model and applied a complementary multi-omics approach to investigate the metabolic profiles under normal and acidified conditions. Our results show that <i>U. prolifera</i> grows at higher rates in acidified environments. Consistently, we observed lower sucrose and phosphocreatine concentrations in response to a higher demand of energy for growth and a higher availability of essential amino acids, likely related to increased protein biosynthesis. In addition, pathways leading to signaling and deterrent compounds appeared perturbed. Finally, a remarkable shift was observed here for the first time in the fatty acid composition of triglycerides, with a decrease in the relative abundance of PUFAs towards an appreciable increase of palmitic acid, thus suggesting a remodeling in lipid biosynthesis. Overall, our studies revealed modulation of several biosynthetic pathways under OA conditions in which, besides the possible effects on the marine ecosystem, the metabolic changes of the alga should be taken into account considering its potential nutraceutical applications. |
| first_indexed | 2024-03-09T16:10:29Z |
| format | Article |
| id | doaj.art-e692d048e40946998a23f560face0656 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T16:10:29Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e692d048e40946998a23f560face06562023-11-24T16:19:25ZengMDPI AGMarine Drugs1660-33972022-11-01201274310.3390/md20120743Responses of the Macroalga <i>Ulva prolifera</i> Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics ApproachesCarlos Sanchez-Arcos0Debora Paris1Valerio Mazzella2Mirko Mutalipassi3Maria Costantini4Maria Cristina Buia5Eric von Elert6Adele Cutignano7Valerio Zupo8Institute for Zoology, Cologne Biocenter University of Cologne, 50674 Köln, GermanyConsiglio Nazionale delle Ricerche (CNR), Istituto di Chimica Biomolecolare (ICB), 80078 Pozzuoli, ItalyDepartment of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Ischia Marine Center, 80077 Ischia, ItalyDepartment of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Calabria Marine Centre, 87071 Amendolara, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, 80121 Napoli, ItalyDepartment of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Ischia Marine Center, 80077 Ischia, ItalyInstitute for Zoology, Cologne Biocenter University of Cologne, 50674 Köln, GermanyConsiglio Nazionale delle Ricerche (CNR), Istituto di Chimica Biomolecolare (ICB), 80078 Pozzuoli, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, 80077 Ischia, ItalyOcean acidification (OA) is a dramatic perturbation of seawater environments due to increasing anthropogenic emissions of CO<sub>2</sub>. Several studies indicated that OA frequently induces marine biota stress and a reduction of biodiversity. Here, we adopted the macroalga <i>Ulva prolifera</i> as a model and applied a complementary multi-omics approach to investigate the metabolic profiles under normal and acidified conditions. Our results show that <i>U. prolifera</i> grows at higher rates in acidified environments. Consistently, we observed lower sucrose and phosphocreatine concentrations in response to a higher demand of energy for growth and a higher availability of essential amino acids, likely related to increased protein biosynthesis. In addition, pathways leading to signaling and deterrent compounds appeared perturbed. Finally, a remarkable shift was observed here for the first time in the fatty acid composition of triglycerides, with a decrease in the relative abundance of PUFAs towards an appreciable increase of palmitic acid, thus suggesting a remodeling in lipid biosynthesis. Overall, our studies revealed modulation of several biosynthetic pathways under OA conditions in which, besides the possible effects on the marine ecosystem, the metabolic changes of the alga should be taken into account considering its potential nutraceutical applications.https://www.mdpi.com/1660-3397/20/12/743ocean acidification (OA)<i>Ulva prolifera</i>metabolomicslipidomicsLC-MSNMR |
| spellingShingle | Carlos Sanchez-Arcos Debora Paris Valerio Mazzella Mirko Mutalipassi Maria Costantini Maria Cristina Buia Eric von Elert Adele Cutignano Valerio Zupo Responses of the Macroalga <i>Ulva prolifera</i> Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics Approaches Marine Drugs ocean acidification (OA) <i>Ulva prolifera</i> metabolomics lipidomics LC-MS NMR |
| title | Responses of the Macroalga <i>Ulva prolifera</i> Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics Approaches |
| title_full | Responses of the Macroalga <i>Ulva prolifera</i> Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics Approaches |
| title_fullStr | Responses of the Macroalga <i>Ulva prolifera</i> Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics Approaches |
| title_full_unstemmed | Responses of the Macroalga <i>Ulva prolifera</i> Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics Approaches |
| title_short | Responses of the Macroalga <i>Ulva prolifera</i> Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics Approaches |
| title_sort | responses of the macroalga i ulva prolifera i muller to ocean acidification revealed by complementary nmr and ms based omics approaches |
| topic | ocean acidification (OA) <i>Ulva prolifera</i> metabolomics lipidomics LC-MS NMR |
| url | https://www.mdpi.com/1660-3397/20/12/743 |
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