Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development
Summary: Thalamus is a critical information relay hub in the cortex; its malfunction causes multiple neurological and psychiatric disorders. However, there are no model systems to study the development and function of human thalamus. Here, we present a protocol to generate regionally specified human...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-06-01
|
| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166719300012 |
| _version_ | 1818212250643595264 |
|---|---|
| author | Yangfei Xiang Bilal Cakir In-Hyun Park |
| author_facet | Yangfei Xiang Bilal Cakir In-Hyun Park |
| author_sort | Yangfei Xiang |
| collection | DOAJ |
| description | Summary: Thalamus is a critical information relay hub in the cortex; its malfunction causes multiple neurological and psychiatric disorders. However, there are no model systems to study the development and function of human thalamus. Here, we present a protocol to generate regionally specified human brain organoids that recapitulate the development of the thalamus using human pluripotent stem cells (hPSCs). Thalamic organoids can be used to study human thalamus development, to model related diseases, and to discover potential therapeutics.For complete information on human thalamic organoids and their application, please refer to the paper by Xiang et al. (2019). |
| first_indexed | 2024-12-12T05:45:25Z |
| format | Article |
| id | doaj.art-e6939e733f994f98bf024dcf5ecfff43 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-12T05:45:25Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-e6939e733f994f98bf024dcf5ecfff432022-12-22T00:35:48ZengElsevierSTAR Protocols2666-16672020-06-0111100001Generation of Regionally Specified Human Brain Organoids Resembling Thalamus DevelopmentYangfei Xiang0Bilal Cakir1In-Hyun Park2Department of Genetics, Yale Stem Cell Center, Yale School of Medicine, New Haven, CT 06520, USA; Corresponding authorDepartment of Genetics, Yale Stem Cell Center, Yale School of Medicine, New Haven, CT 06520, USADepartment of Genetics, Yale Stem Cell Center, Yale School of Medicine, New Haven, CT 06520, USA; Corresponding authorSummary: Thalamus is a critical information relay hub in the cortex; its malfunction causes multiple neurological and psychiatric disorders. However, there are no model systems to study the development and function of human thalamus. Here, we present a protocol to generate regionally specified human brain organoids that recapitulate the development of the thalamus using human pluripotent stem cells (hPSCs). Thalamic organoids can be used to study human thalamus development, to model related diseases, and to discover potential therapeutics.For complete information on human thalamic organoids and their application, please refer to the paper by Xiang et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166719300012 |
| spellingShingle | Yangfei Xiang Bilal Cakir In-Hyun Park Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development STAR Protocols |
| title | Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development |
| title_full | Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development |
| title_fullStr | Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development |
| title_full_unstemmed | Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development |
| title_short | Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development |
| title_sort | generation of regionally specified human brain organoids resembling thalamus development |
| url | http://www.sciencedirect.com/science/article/pii/S2666166719300012 |
| work_keys_str_mv | AT yangfeixiang generationofregionallyspecifiedhumanbrainorganoidsresemblingthalamusdevelopment AT bilalcakir generationofregionallyspecifiedhumanbrainorganoidsresemblingthalamusdevelopment AT inhyunpark generationofregionallyspecifiedhumanbrainorganoidsresemblingthalamusdevelopment |