Cordycepin inhibits myogenesis via activating the ERK1/2 MAPK signalling pathway in C2C12 cells
Cordycepin (with a molecular formula of C10H13N5O3), a natural adenosine isolated from Cordyceps militaris, has an important regulatory effect on skeletal muscle remodelling and quality maintenance. The aim of this study was to investigate the effect of cordycepin on myoblast differentiation and exp...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S075333222300954X |
| _version_ | 1797744096774717440 |
|---|---|
| author | Chunfang Cheng Shasha Zhang Yanchun Gong Xuanyu Wang Shan Tang Juan Wan Kaizhi Ding Chunhua Yuan Wei Sun Li-Hua Yao |
| author_facet | Chunfang Cheng Shasha Zhang Yanchun Gong Xuanyu Wang Shan Tang Juan Wan Kaizhi Ding Chunhua Yuan Wei Sun Li-Hua Yao |
| author_sort | Chunfang Cheng |
| collection | DOAJ |
| description | Cordycepin (with a molecular formula of C10H13N5O3), a natural adenosine isolated from Cordyceps militaris, has an important regulatory effect on skeletal muscle remodelling and quality maintenance. The aim of this study was to investigate the effect of cordycepin on myoblast differentiation and explore the underlying molecular mechanisms of this effect. Our results showed that cordycepin inhibited myogenesis by downregulating myogenic differentiation (MyoD) and myogenin (MyoG), preserved undifferentiated reserve cell pools by upregulating myogenic factor 5 (Myf5) and retinoblastoma-like protein p130 (p130), and enhanced energy reserves by decreasing intracellular reactive oxygen species (ROS) and enhancing mitochondrial membrane potential, mitochondrial mass, and ATP content. The effect of cordycepin on myogenesis was associated with increased phosphorylation of extracellular signal-regulated kinase 1/2 (p-ERK1/2). PD98059 (a specific inhibitor of p-ERK1/2) attenuated the inhibitory effect of cordycepin on C2C12 differentiation. The present study reveals that cordycepin inhibits myogenesis through ERK1/2 MAPK signalling activation accompanied by an increase in skeletal muscle energy reserves and improving skeletal muscle oxidative stress, which may have implications for its further application for the prevention and treatment of degenerative muscle diseases caused by the depletion of depleted muscle stem cells. |
| first_indexed | 2024-03-12T15:04:50Z |
| format | Article |
| id | doaj.art-e693d6bf8fe6481c804006dd62c4646e |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-12T15:04:50Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-e693d6bf8fe6481c804006dd62c4646e2023-08-13T04:52:49ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-09-01165115163Cordycepin inhibits myogenesis via activating the ERK1/2 MAPK signalling pathway in C2C12 cellsChunfang Cheng0Shasha Zhang1Yanchun Gong2Xuanyu Wang3Shan Tang4Juan Wan5Kaizhi Ding6Chunhua Yuan7Wei Sun8Li-Hua Yao9School of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR ChinaSchool of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR ChinaSchool of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China; School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR ChinaSchool of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR ChinaSchool of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR ChinaSchool of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR ChinaSchool of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR ChinaSchool of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR ChinaSchool of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR ChinaSchool of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China; School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China; Corresponding author at: School of Life Science, Jiangxi Science & Technology Normal University, Nanchang 330013, PR China.Cordycepin (with a molecular formula of C10H13N5O3), a natural adenosine isolated from Cordyceps militaris, has an important regulatory effect on skeletal muscle remodelling and quality maintenance. The aim of this study was to investigate the effect of cordycepin on myoblast differentiation and explore the underlying molecular mechanisms of this effect. Our results showed that cordycepin inhibited myogenesis by downregulating myogenic differentiation (MyoD) and myogenin (MyoG), preserved undifferentiated reserve cell pools by upregulating myogenic factor 5 (Myf5) and retinoblastoma-like protein p130 (p130), and enhanced energy reserves by decreasing intracellular reactive oxygen species (ROS) and enhancing mitochondrial membrane potential, mitochondrial mass, and ATP content. The effect of cordycepin on myogenesis was associated with increased phosphorylation of extracellular signal-regulated kinase 1/2 (p-ERK1/2). PD98059 (a specific inhibitor of p-ERK1/2) attenuated the inhibitory effect of cordycepin on C2C12 differentiation. The present study reveals that cordycepin inhibits myogenesis through ERK1/2 MAPK signalling activation accompanied by an increase in skeletal muscle energy reserves and improving skeletal muscle oxidative stress, which may have implications for its further application for the prevention and treatment of degenerative muscle diseases caused by the depletion of depleted muscle stem cells.http://www.sciencedirect.com/science/article/pii/S075333222300954XCordycepinC2C12 cellsMyogenesisERK1/2 MAPKReserve cell |
| spellingShingle | Chunfang Cheng Shasha Zhang Yanchun Gong Xuanyu Wang Shan Tang Juan Wan Kaizhi Ding Chunhua Yuan Wei Sun Li-Hua Yao Cordycepin inhibits myogenesis via activating the ERK1/2 MAPK signalling pathway in C2C12 cells Biomedicine & Pharmacotherapy Cordycepin C2C12 cells Myogenesis ERK1/2 MAPK Reserve cell |
| title | Cordycepin inhibits myogenesis via activating the ERK1/2 MAPK signalling pathway in C2C12 cells |
| title_full | Cordycepin inhibits myogenesis via activating the ERK1/2 MAPK signalling pathway in C2C12 cells |
| title_fullStr | Cordycepin inhibits myogenesis via activating the ERK1/2 MAPK signalling pathway in C2C12 cells |
| title_full_unstemmed | Cordycepin inhibits myogenesis via activating the ERK1/2 MAPK signalling pathway in C2C12 cells |
| title_short | Cordycepin inhibits myogenesis via activating the ERK1/2 MAPK signalling pathway in C2C12 cells |
| title_sort | cordycepin inhibits myogenesis via activating the erk1 2 mapk signalling pathway in c2c12 cells |
| topic | Cordycepin C2C12 cells Myogenesis ERK1/2 MAPK Reserve cell |
| url | http://www.sciencedirect.com/science/article/pii/S075333222300954X |
| work_keys_str_mv | AT chunfangcheng cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells AT shashazhang cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells AT yanchungong cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells AT xuanyuwang cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells AT shantang cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells AT juanwan cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells AT kaizhiding cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells AT chunhuayuan cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells AT weisun cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells AT lihuayao cordycepininhibitsmyogenesisviaactivatingtheerk12mapksignallingpathwayinc2c12cells |