The paeonol target gene autophagy-related 5 has a potential therapeutic value in psoriasis treatment
Background Paeonol is a potent therapy for psoriasis. This study aimed to screen out paeonol-targeted genes in psoriasis and validate the potential of using paeonol for the management of psoriasis. Methods Microarray datasets were obtained from the Gene Expression Omnibus. The differentially express...
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PeerJ Inc.
2021-05-01
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| Online Access: | https://peerj.com/articles/11278.pdf |
| _version_ | 1797423874275540992 |
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| author | Qian Zhang Hongqiao Shi Jiaan Zhang Chenxue Jiang Chunxiang Zhou |
| author_facet | Qian Zhang Hongqiao Shi Jiaan Zhang Chenxue Jiang Chunxiang Zhou |
| author_sort | Qian Zhang |
| collection | DOAJ |
| description | Background Paeonol is a potent therapy for psoriasis. This study aimed to screen out paeonol-targeted genes in psoriasis and validate the potential of using paeonol for the management of psoriasis. Methods Microarray datasets were obtained from the Gene Expression Omnibus. The differentially expressed genes (DEGs) in the lesional skin samples and the overlapping genes between DEGs and paeonol- and psoriasis-related genes were defined as potential targets for psoriasis. After being treated with si-ATG5 and pc-ATG5, human HaCaT cells were treated with 100 ng/ml IL-22 and 10 ng/ml TNF-α with and without paeonol. Cell proliferation, apoptosis, and expression of interleukin (IL)-6, IL-1β, Beclin 1, ATG5, and p62 in HaCaT cells were determined using ESLIA, PCR, and Western blot analysis. Results A total of 779 DEGs were identified in the lesional skin samples compared with the non-lesional tissues. The autophagy-related 5 (ATG5) gene was the only gene that overlapped between the DEGs and genes related to paeonol and psoriasis. Cell proliferation, inflammatory cytokines (IL-6 and IL-1β), and ATG5 expression were increased in IL-22/TNF-α-stimulated HaCaT (model) cells compared with control. Paeonol treatment rescued all changes. si-ATG5 transfection increased inflammation and apoptosis in model cells compared with controls. pc-ATG5 prevented IL-22/TNF-α-induced changes in HaCaT cells. Also, si-ATG5 decreased p62 and Beclin 1 proteins, while pc-ATG5 increased them both. Conclusions ATG5-dependent autophagy plays a crucial role in psoriasis. The ATG5 gene might be a therapeutic target for the management of in vitro psoriasis. |
| first_indexed | 2024-03-09T07:54:04Z |
| format | Article |
| id | doaj.art-e696bdd0c613472e8d921985497b1e34 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:54:04Z |
| publishDate | 2021-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-e696bdd0c613472e8d921985497b1e342023-12-03T01:22:08ZengPeerJ Inc.PeerJ2167-83592021-05-019e1127810.7717/peerj.11278The paeonol target gene autophagy-related 5 has a potential therapeutic value in psoriasis treatmentQian Zhang0Hongqiao Shi1Jiaan Zhang2Chenxue Jiang3Chunxiang Zhou4Department of Dermatology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, ChinaDepartment of Dermatology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, ChinaInstitute of Dermatology, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs,Chinese Academy of Medical Science&Peking Union Medical College, Nanjing, Jiangsu Province, ChinaSchool of Foreign Languages, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, ChinaCollege of Traditional Chinese Medicine and College of Integrated Chinese and Western Medicine, Nanjing, Jiangsu Province, ChinaBackground Paeonol is a potent therapy for psoriasis. This study aimed to screen out paeonol-targeted genes in psoriasis and validate the potential of using paeonol for the management of psoriasis. Methods Microarray datasets were obtained from the Gene Expression Omnibus. The differentially expressed genes (DEGs) in the lesional skin samples and the overlapping genes between DEGs and paeonol- and psoriasis-related genes were defined as potential targets for psoriasis. After being treated with si-ATG5 and pc-ATG5, human HaCaT cells were treated with 100 ng/ml IL-22 and 10 ng/ml TNF-α with and without paeonol. Cell proliferation, apoptosis, and expression of interleukin (IL)-6, IL-1β, Beclin 1, ATG5, and p62 in HaCaT cells were determined using ESLIA, PCR, and Western blot analysis. Results A total of 779 DEGs were identified in the lesional skin samples compared with the non-lesional tissues. The autophagy-related 5 (ATG5) gene was the only gene that overlapped between the DEGs and genes related to paeonol and psoriasis. Cell proliferation, inflammatory cytokines (IL-6 and IL-1β), and ATG5 expression were increased in IL-22/TNF-α-stimulated HaCaT (model) cells compared with control. Paeonol treatment rescued all changes. si-ATG5 transfection increased inflammation and apoptosis in model cells compared with controls. pc-ATG5 prevented IL-22/TNF-α-induced changes in HaCaT cells. Also, si-ATG5 decreased p62 and Beclin 1 proteins, while pc-ATG5 increased them both. Conclusions ATG5-dependent autophagy plays a crucial role in psoriasis. The ATG5 gene might be a therapeutic target for the management of in vitro psoriasis.https://peerj.com/articles/11278.pdfAutophagy related 5PsoriasisDifferentially expressed genesAutophagyPaeonol |
| spellingShingle | Qian Zhang Hongqiao Shi Jiaan Zhang Chenxue Jiang Chunxiang Zhou The paeonol target gene autophagy-related 5 has a potential therapeutic value in psoriasis treatment PeerJ Autophagy related 5 Psoriasis Differentially expressed genes Autophagy Paeonol |
| title | The paeonol target gene autophagy-related 5 has a potential therapeutic value in psoriasis treatment |
| title_full | The paeonol target gene autophagy-related 5 has a potential therapeutic value in psoriasis treatment |
| title_fullStr | The paeonol target gene autophagy-related 5 has a potential therapeutic value in psoriasis treatment |
| title_full_unstemmed | The paeonol target gene autophagy-related 5 has a potential therapeutic value in psoriasis treatment |
| title_short | The paeonol target gene autophagy-related 5 has a potential therapeutic value in psoriasis treatment |
| title_sort | paeonol target gene autophagy related 5 has a potential therapeutic value in psoriasis treatment |
| topic | Autophagy related 5 Psoriasis Differentially expressed genes Autophagy Paeonol |
| url | https://peerj.com/articles/11278.pdf |
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