A Novel R2R3-MYB Transcription Factor <i>Sb</i>MYB12 Positively Regulates Baicalin Biosynthesis in <i>Scutellaria baicalensis</i> Georgi
<i>Scutellaria baicalensis</i> Georgi is an annual herb from the <i>Scutellaria</i> genus that has been extensively used as a traditional medicine for over 2000 years in China. Baicalin and other flavonoids have been identified as the principal bioactive ingredients. The bios...
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/15452 |
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| author | Wentao Wang Suying Hu Jing Yang Caijuan Zhang Tong Zhang Donghao Wang Xiaoyan Cao Zhezhi Wang |
| author_facet | Wentao Wang Suying Hu Jing Yang Caijuan Zhang Tong Zhang Donghao Wang Xiaoyan Cao Zhezhi Wang |
| author_sort | Wentao Wang |
| collection | DOAJ |
| description | <i>Scutellaria baicalensis</i> Georgi is an annual herb from the <i>Scutellaria</i> genus that has been extensively used as a traditional medicine for over 2000 years in China. Baicalin and other flavonoids have been identified as the principal bioactive ingredients. The biosynthetic pathway of baicalin in <i>S. baicalensis</i> has been elucidated; however, the specific functions of R2R3-MYB TF, which regulates baicalin synthesis, has not been well characterized in <i>S. baicalensis</i> to date. Here, a S20 R2R3-MYB TF (<i>Sb</i>MYB12), which encodes 263 amino acids with a length of 792 bp, was expressed in all tested tissues (mainly in leaves) and responded to exogenous hormone methyl jasmonate (MeJA) treatment. The overexpression of <i>SbMYB12</i> significantly promoted the accumulation of flavonoids such as baicalin and wogonoside in <i>S. baicalensis</i> hairy roots. Furthermore, biochemical experiments revealed that <i>Sb</i>MYB12 is a nuclear-localized transcription activator that binds to the <i>SbCCL7-4</i>, <i>SbCHI-2</i>, and <i>SbF6H-1</i> promoters to activate their expression. These results illustrate that <i>SbMYB12</i> positively regulates the generation of baicalin and wogonoside. In summary, this work revealed a novel S20 R2R3-MYB regulator and enhances our understanding of the transcriptional and regulatory mechanisms of baicalin biosynthesis, as well as sheds new light on metabolic engineering in <i>S. baicalensis</i>. |
| first_indexed | 2024-03-09T16:21:10Z |
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| last_indexed | 2024-03-09T16:21:10Z |
| publishDate | 2022-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e698c51f38014464a26bc973e12b81532023-11-24T15:22:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241545210.3390/ijms232415452A Novel R2R3-MYB Transcription Factor <i>Sb</i>MYB12 Positively Regulates Baicalin Biosynthesis in <i>Scutellaria baicalensis</i> GeorgiWentao Wang0Suying Hu1Jing Yang2Caijuan Zhang3Tong Zhang4Donghao Wang5Xiaoyan Cao6Zhezhi Wang7National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an 710062, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an 710062, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an 710062, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an 710062, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an 710062, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an 710062, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an 710062, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an 710062, China<i>Scutellaria baicalensis</i> Georgi is an annual herb from the <i>Scutellaria</i> genus that has been extensively used as a traditional medicine for over 2000 years in China. Baicalin and other flavonoids have been identified as the principal bioactive ingredients. The biosynthetic pathway of baicalin in <i>S. baicalensis</i> has been elucidated; however, the specific functions of R2R3-MYB TF, which regulates baicalin synthesis, has not been well characterized in <i>S. baicalensis</i> to date. Here, a S20 R2R3-MYB TF (<i>Sb</i>MYB12), which encodes 263 amino acids with a length of 792 bp, was expressed in all tested tissues (mainly in leaves) and responded to exogenous hormone methyl jasmonate (MeJA) treatment. The overexpression of <i>SbMYB12</i> significantly promoted the accumulation of flavonoids such as baicalin and wogonoside in <i>S. baicalensis</i> hairy roots. Furthermore, biochemical experiments revealed that <i>Sb</i>MYB12 is a nuclear-localized transcription activator that binds to the <i>SbCCL7-4</i>, <i>SbCHI-2</i>, and <i>SbF6H-1</i> promoters to activate their expression. These results illustrate that <i>SbMYB12</i> positively regulates the generation of baicalin and wogonoside. In summary, this work revealed a novel S20 R2R3-MYB regulator and enhances our understanding of the transcriptional and regulatory mechanisms of baicalin biosynthesis, as well as sheds new light on metabolic engineering in <i>S. baicalensis</i>.https://www.mdpi.com/1422-0067/23/24/15452<i>Scutellaria baicalensis</i>R2R3-MYBSbMYB12flavonoids biosynthesisbaicalinpositive regulation |
| spellingShingle | Wentao Wang Suying Hu Jing Yang Caijuan Zhang Tong Zhang Donghao Wang Xiaoyan Cao Zhezhi Wang A Novel R2R3-MYB Transcription Factor <i>Sb</i>MYB12 Positively Regulates Baicalin Biosynthesis in <i>Scutellaria baicalensis</i> Georgi International Journal of Molecular Sciences <i>Scutellaria baicalensis</i> R2R3-MYB SbMYB12 flavonoids biosynthesis baicalin positive regulation |
| title | A Novel R2R3-MYB Transcription Factor <i>Sb</i>MYB12 Positively Regulates Baicalin Biosynthesis in <i>Scutellaria baicalensis</i> Georgi |
| title_full | A Novel R2R3-MYB Transcription Factor <i>Sb</i>MYB12 Positively Regulates Baicalin Biosynthesis in <i>Scutellaria baicalensis</i> Georgi |
| title_fullStr | A Novel R2R3-MYB Transcription Factor <i>Sb</i>MYB12 Positively Regulates Baicalin Biosynthesis in <i>Scutellaria baicalensis</i> Georgi |
| title_full_unstemmed | A Novel R2R3-MYB Transcription Factor <i>Sb</i>MYB12 Positively Regulates Baicalin Biosynthesis in <i>Scutellaria baicalensis</i> Georgi |
| title_short | A Novel R2R3-MYB Transcription Factor <i>Sb</i>MYB12 Positively Regulates Baicalin Biosynthesis in <i>Scutellaria baicalensis</i> Georgi |
| title_sort | novel r2r3 myb transcription factor i sb i myb12 positively regulates baicalin biosynthesis in i scutellaria baicalensis i georgi |
| topic | <i>Scutellaria baicalensis</i> R2R3-MYB SbMYB12 flavonoids biosynthesis baicalin positive regulation |
| url | https://www.mdpi.com/1422-0067/23/24/15452 |
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