Enumeration, isolation, and identification of bifidobacteria from dairy products
Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg l–1). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were char...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Ljubljana Press (Založba Univerze v Ljubljani)
2004-12-01
|
| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/15422 |
| _version_ | 1826532200044036096 |
|---|---|
| author | Eva VLKOVÁ Vojtěch RADA Iva TROJANOVÁ |
| author_facet | Eva VLKOVÁ Vojtěch RADA Iva TROJANOVÁ |
| author_sort | Eva VLKOVÁ |
| collection | DOAJ |
| description | Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg l–1). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46 °C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification was also carried out by PCR method using genus- and species-specific primers. The bifidobacterial counts in products tested varied from 2.37 to 7.17 log CFU/ml. High selectivity was seen for modified TPY agar from which all isolates were identified as bifidobacteria. While most strains were identified as B. animalis using Bacter program, the same isolates had positive reaction with B. lactis-specific primers. Strain isolated from Rajo yogurt was identified as B. longum by Bacter as well as using PCR, but its bacterial counts were too low. Our results showed, that most of the bifidobacterial strains currently used in food products could be of animal origin. |
| first_indexed | 2024-03-08T23:23:15Z |
| format | Article |
| id | doaj.art-e6a2277a84574187847ea164b2da877c |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2025-03-14T01:47:27Z |
| publishDate | 2004-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-e6a2277a84574187847ea164b2da877c2025-03-12T12:22:22ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412004-12-01841313610.14720/aas.2004.84.1.1542221815Enumeration, isolation, and identification of bifidobacteria from dairy productsEva VLKOVÁ0Vojtěch RADA1Iva TROJANOVÁ2Department of Microbiology and Biotechnology, Czech University of Agriculture Prague, Kamýcká 129, 165 21 Prague 6, Czech RepublicDepartment of Microbiology and Biotechnology, Czech University of Agriculture Prague, Kamýcká 129, 165 21 Prague 6, Czech RepublicDepartment of Microbiology and Biotechnology, Czech University of Agriculture Prague, Kamýcká 129, 165 21 Prague 6, Czech RepublicSix dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg l–1). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46 °C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification was also carried out by PCR method using genus- and species-specific primers. The bifidobacterial counts in products tested varied from 2.37 to 7.17 log CFU/ml. High selectivity was seen for modified TPY agar from which all isolates were identified as bifidobacteria. While most strains were identified as B. animalis using Bacter program, the same isolates had positive reaction with B. lactis-specific primers. Strain isolated from Rajo yogurt was identified as B. longum by Bacter as well as using PCR, but its bacterial counts were too low. Our results showed, that most of the bifidobacterial strains currently used in food products could be of animal origin.https://journals.uni-lj.si/aas/article/view/15422milk productsdairy productsmicrobiologybifidobacteriaenumerationidentificationisolation |
| spellingShingle | Eva VLKOVÁ Vojtěch RADA Iva TROJANOVÁ Enumeration, isolation, and identification of bifidobacteria from dairy products Acta Agriculturae Slovenica milk products dairy products microbiology bifidobacteria enumeration identification isolation |
| title | Enumeration, isolation, and identification of bifidobacteria from dairy products |
| title_full | Enumeration, isolation, and identification of bifidobacteria from dairy products |
| title_fullStr | Enumeration, isolation, and identification of bifidobacteria from dairy products |
| title_full_unstemmed | Enumeration, isolation, and identification of bifidobacteria from dairy products |
| title_short | Enumeration, isolation, and identification of bifidobacteria from dairy products |
| title_sort | enumeration isolation and identification of bifidobacteria from dairy products |
| topic | milk products dairy products microbiology bifidobacteria enumeration identification isolation |
| url | https://journals.uni-lj.si/aas/article/view/15422 |
| work_keys_str_mv | AT evavlkova enumerationisolationandidentificationofbifidobacteriafromdairyproducts AT vojtechrada enumerationisolationandidentificationofbifidobacteriafromdairyproducts AT ivatrojanova enumerationisolationandidentificationofbifidobacteriafromdairyproducts |