Functional Identification of <i>HhUGT74AG11</i>—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in <i>Hedera helix</i>
<i>Hedera helix</i> is a traditional medicinal plant. Its primary active ingredients are oleanane-type saponins, which have extensive pharmacological effects such as gastric mucosal protection, autophagy regulation actions, and antiviral properties. However, the glycosylation-modifying e...
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2024-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/7/4067 |
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| author | Han Yu Jun Zhou Jing Zhang Xinyi He Siqing Peng Hao Ling Zhuang Dong Xiangyang Lu Yun Tian Guiping Guan Qi Tang Xiaohong Zhong Yuedong He |
| author_facet | Han Yu Jun Zhou Jing Zhang Xinyi He Siqing Peng Hao Ling Zhuang Dong Xiangyang Lu Yun Tian Guiping Guan Qi Tang Xiaohong Zhong Yuedong He |
| author_sort | Han Yu |
| collection | DOAJ |
| description | <i>Hedera helix</i> is a traditional medicinal plant. Its primary active ingredients are oleanane-type saponins, which have extensive pharmacological effects such as gastric mucosal protection, autophagy regulation actions, and antiviral properties. However, the glycosylation-modifying enzymes responsible for catalyzing oleanane-type saponin biosynthesis remain unidentified. Through transcriptome, cluster analysis, and PSPG structural domain, this study preliminarily screened four candidate UDP-glycosyltransferases (UGTs), including Unigene26859, Unigene31717, CL11391.Contig2, and CL144.Contig9. In in vitro enzymatic reactions, it has been observed that Unigene26859 (<i>HhUGT74AG11</i>) has the ability to facilitate the conversion of oleanolic acid, resulting in the production of oleanolic acid 28-<i>O</i>-glucopyranosyl ester. Moreover, <i>HhUGT74AG11</i> exhibits extensive substrate hybridity and specific stereoselectivity and can transfer glycosyl donors to the <i>C</i>-28 site of various oleanane-type triterpenoids (hederagenin and calenduloside E) and the <i>C</i>-7 site of flavonoids (tectorigenin). Cluster analysis found that <i>HhUGT74AG11</i> is clustered together with functionally identified genes <i>AeUGT74AG6</i>, <i>CaUGT74AG2</i>, and <i>PgUGT74AE2</i>, further verifying the possible reason for <i>HhUGT74AG11</i> catalyzing substrate generalization. In this study, a novel glycosyltransferase, <i>HhUGT74AG11</i>, was characterized that plays a role in oleanane-type saponins biosynthesis in <i>H. helix,</i> providing a theoretical basis for the production of rare and valuable triterpenoid saponins. |
| first_indexed | 2024-04-24T10:42:48Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T10:42:48Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e6a2e29ee0924190a7e0ac4f028f681a2024-04-12T13:20:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-04-01257406710.3390/ijms25074067Functional Identification of <i>HhUGT74AG11</i>—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in <i>Hedera helix</i>Han Yu0Jun Zhou1Jing Zhang2Xinyi He3Siqing Peng4Hao Ling5Zhuang Dong6Xiangyang Lu7Yun Tian8Guiping Guan9Qi Tang10Xiaohong Zhong11Yuedong He12College of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<i>Hedera helix</i> is a traditional medicinal plant. Its primary active ingredients are oleanane-type saponins, which have extensive pharmacological effects such as gastric mucosal protection, autophagy regulation actions, and antiviral properties. However, the glycosylation-modifying enzymes responsible for catalyzing oleanane-type saponin biosynthesis remain unidentified. Through transcriptome, cluster analysis, and PSPG structural domain, this study preliminarily screened four candidate UDP-glycosyltransferases (UGTs), including Unigene26859, Unigene31717, CL11391.Contig2, and CL144.Contig9. In in vitro enzymatic reactions, it has been observed that Unigene26859 (<i>HhUGT74AG11</i>) has the ability to facilitate the conversion of oleanolic acid, resulting in the production of oleanolic acid 28-<i>O</i>-glucopyranosyl ester. Moreover, <i>HhUGT74AG11</i> exhibits extensive substrate hybridity and specific stereoselectivity and can transfer glycosyl donors to the <i>C</i>-28 site of various oleanane-type triterpenoids (hederagenin and calenduloside E) and the <i>C</i>-7 site of flavonoids (tectorigenin). Cluster analysis found that <i>HhUGT74AG11</i> is clustered together with functionally identified genes <i>AeUGT74AG6</i>, <i>CaUGT74AG2</i>, and <i>PgUGT74AE2</i>, further verifying the possible reason for <i>HhUGT74AG11</i> catalyzing substrate generalization. In this study, a novel glycosyltransferase, <i>HhUGT74AG11</i>, was characterized that plays a role in oleanane-type saponins biosynthesis in <i>H. helix,</i> providing a theoretical basis for the production of rare and valuable triterpenoid saponins.https://www.mdpi.com/1422-0067/25/7/4067UDP-glycosyltransferasesubstrate hybriditybiosynthesisoleanane-type saponin<i>Hedera helix</i> |
| spellingShingle | Han Yu Jun Zhou Jing Zhang Xinyi He Siqing Peng Hao Ling Zhuang Dong Xiangyang Lu Yun Tian Guiping Guan Qi Tang Xiaohong Zhong Yuedong He Functional Identification of <i>HhUGT74AG11</i>—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in <i>Hedera helix</i> International Journal of Molecular Sciences UDP-glycosyltransferase substrate hybridity biosynthesis oleanane-type saponin <i>Hedera helix</i> |
| title | Functional Identification of <i>HhUGT74AG11</i>—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in <i>Hedera helix</i> |
| title_full | Functional Identification of <i>HhUGT74AG11</i>—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in <i>Hedera helix</i> |
| title_fullStr | Functional Identification of <i>HhUGT74AG11</i>—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in <i>Hedera helix</i> |
| title_full_unstemmed | Functional Identification of <i>HhUGT74AG11</i>—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in <i>Hedera helix</i> |
| title_short | Functional Identification of <i>HhUGT74AG11</i>—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in <i>Hedera helix</i> |
| title_sort | functional identification of i hhugt74ag11 i a key glycosyltransferase involved in biosynthesis of oleanane type saponins in i hedera helix i |
| topic | UDP-glycosyltransferase substrate hybridity biosynthesis oleanane-type saponin <i>Hedera helix</i> |
| url | https://www.mdpi.com/1422-0067/25/7/4067 |
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