Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis
AbstractNanostructures composed of liposomes and polydopamine (PDA) have demonstrated efficacy as carriers for delivering plasmids, effectively alleviating renal cell carcinoma. However, their role in acute kidney injury (AKI) remains unclear. This study aimed to investigate the effects of the plasm...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Renal Failure |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2319327 |
| _version_ | 1797230964486701056 |
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| author | Rongjie Zhang Xin Zhang Xuhui Zhu Tao Li Yansheng Li Peng Zhang Yuanhao Chen Gao Li Xiuwu Han |
| author_facet | Rongjie Zhang Xin Zhang Xuhui Zhu Tao Li Yansheng Li Peng Zhang Yuanhao Chen Gao Li Xiuwu Han |
| author_sort | Rongjie Zhang |
| collection | DOAJ |
| description | AbstractNanostructures composed of liposomes and polydopamine (PDA) have demonstrated efficacy as carriers for delivering plasmids, effectively alleviating renal cell carcinoma. However, their role in acute kidney injury (AKI) remains unclear. This study aimed to investigate the effects of the plasmid-encoded lncRNA-OIP5-AS1@PDA nanoparticles (POP-NPs) on renal ischemia/reperfusion (RI/R) injury and explore the underlying mechanisms. RI/R or OGD/R models were established in mice and HK-2 cells, respectively. In vivo, vector or POP-NPs were administered (10 nmol, IV) 48 h after RI/R treatment. In the RI/R mouse model, the OIP5-AS1 and Nrf2/HO-1 expressions were down-regulated, while miR-410-3p expression was upregulated. POP-NPs treatment effectively reversed RI/R-induced renal tissue injury, restoring altered levels of blood urea nitrogen, creatinine, malondialdehyde, inflammatory factors (IL-8, IL-6, TNF-α), ROS, apoptosis, miR-410-3p, as well as the suppressed expression of SOD and Nrf2/HO-1 in the model mice. Similar results were obtained in cell models treated with POP-NPs. Additionally, miR-410-3p mimics could reverse the effects of POP-NPs on cellular models, partially counteracted by Nrf2 agonists. The binding relationship between OIP5-AS1 and miR-410-3p, alongside miR-410-3p and Nrf2, has been substantiated by dual-luciferase reporter and RNA pull-down assays. The study revealed that POP-NPs can attenuate RI/R-induced injury through miR-410-3p/Nrf2 axis. These findings lay the groundwork for future targeted therapeutic approaches utilizing nanoparticles for RI/R-induced AKI. |
| first_indexed | 2024-03-07T19:29:25Z |
| format | Article |
| id | doaj.art-e6a53c4e24504a7b80bc35e695e04be7 |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-04-24T15:36:51Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-e6a53c4e24504a7b80bc35e695e04be72024-04-02T01:32:00ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492024-12-0146110.1080/0886022X.2024.2319327Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axisRongjie Zhang0Xin Zhang1Xuhui Zhu2Tao Li3Yansheng Li4Peng Zhang5Yuanhao Chen6Gao Li7Xiuwu Han8Department of Urology, Beijing Chao-Yang Hospital, Beijing, ChinaDepartment of Urology, Beijing Chao-Yang Hospital, Beijing, ChinaDepartment of Urology, Beijing Chao-Yang Hospital, Beijing, ChinaDepartment of Urology, Beijing Chao-Yang Hospital, Beijing, ChinaDepartment of Urology, Beijing Chao-Yang Hospital, Beijing, ChinaDepartment of Urology, Beijing Chao-Yang Hospital, Beijing, ChinaDepartment of Urology, Beijing Chao-Yang Hospital, Beijing, ChinaDepartment of Urology, Beijing Chao-Yang Hospital, Beijing, ChinaDepartment of Urology, Beijing Chao-Yang Hospital, Beijing, ChinaAbstractNanostructures composed of liposomes and polydopamine (PDA) have demonstrated efficacy as carriers for delivering plasmids, effectively alleviating renal cell carcinoma. However, their role in acute kidney injury (AKI) remains unclear. This study aimed to investigate the effects of the plasmid-encoded lncRNA-OIP5-AS1@PDA nanoparticles (POP-NPs) on renal ischemia/reperfusion (RI/R) injury and explore the underlying mechanisms. RI/R or OGD/R models were established in mice and HK-2 cells, respectively. In vivo, vector or POP-NPs were administered (10 nmol, IV) 48 h after RI/R treatment. In the RI/R mouse model, the OIP5-AS1 and Nrf2/HO-1 expressions were down-regulated, while miR-410-3p expression was upregulated. POP-NPs treatment effectively reversed RI/R-induced renal tissue injury, restoring altered levels of blood urea nitrogen, creatinine, malondialdehyde, inflammatory factors (IL-8, IL-6, TNF-α), ROS, apoptosis, miR-410-3p, as well as the suppressed expression of SOD and Nrf2/HO-1 in the model mice. Similar results were obtained in cell models treated with POP-NPs. Additionally, miR-410-3p mimics could reverse the effects of POP-NPs on cellular models, partially counteracted by Nrf2 agonists. The binding relationship between OIP5-AS1 and miR-410-3p, alongside miR-410-3p and Nrf2, has been substantiated by dual-luciferase reporter and RNA pull-down assays. The study revealed that POP-NPs can attenuate RI/R-induced injury through miR-410-3p/Nrf2 axis. These findings lay the groundwork for future targeted therapeutic approaches utilizing nanoparticles for RI/R-induced AKI.https://www.tandfonline.com/doi/10.1080/0886022X.2024.2319327Plasmid-encoded lncRNA-OIP5-AS1@PDA nanoparticles (POP-NPs)lncRNA OIP5-AS1miR-410-3pNrf2/HO-1 signaling pathwayrenal ischemia/reperfusion injury |
| spellingShingle | Rongjie Zhang Xin Zhang Xuhui Zhu Tao Li Yansheng Li Peng Zhang Yuanhao Chen Gao Li Xiuwu Han Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis Renal Failure Plasmid-encoded lncRNA-OIP5-AS1@PDA nanoparticles (POP-NPs) lncRNA OIP5-AS1 miR-410-3p Nrf2/HO-1 signaling pathway renal ischemia/reperfusion injury |
| title | Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis |
| title_full | Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis |
| title_fullStr | Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis |
| title_full_unstemmed | Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis |
| title_short | Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis |
| title_sort | nanoparticles transfected with plasmid encoded lncrna oip5 as1 inhibit renal ischemia reperfusion injury in mice via the mir 410 3p nrf2 axis |
| topic | Plasmid-encoded lncRNA-OIP5-AS1@PDA nanoparticles (POP-NPs) lncRNA OIP5-AS1 miR-410-3p Nrf2/HO-1 signaling pathway renal ischemia/reperfusion injury |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2319327 |
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