Peli1 signaling blockade attenuates congenital zika syndrome.
Zika virus (ZIKV) infects pregnant women and causes devastating congenital zika syndrome (CZS). How the virus is vertically transmitted to the fetus and induces neuronal loss remains unclear. We previously reported that Pellino (Peli)1, an E3 ubiquitin ligase, promotes p38MAPK activation in microgli...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-06-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1008538 |
| _version_ | 1819045162630774784 |
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| author | Huanle Luo Guangyu Li Binbin Wang Bing Tian Junling Gao Jing Zou Shuizhen Shi Shuang Zhu Bi-Hung Peng Awadalkareem Adam Ariza Martinez Kimberly Hein Evandro R Winkelmann Yoseph Mahmoud Xiaofei Zhou Chao Shan Shannan Rossi Scott Weaver Alan D T Barrett Shao-Cong Sun Wenbo Zhang Pei-Yong Shi Ping Wu Tian Wang |
| author_facet | Huanle Luo Guangyu Li Binbin Wang Bing Tian Junling Gao Jing Zou Shuizhen Shi Shuang Zhu Bi-Hung Peng Awadalkareem Adam Ariza Martinez Kimberly Hein Evandro R Winkelmann Yoseph Mahmoud Xiaofei Zhou Chao Shan Shannan Rossi Scott Weaver Alan D T Barrett Shao-Cong Sun Wenbo Zhang Pei-Yong Shi Ping Wu Tian Wang |
| author_sort | Huanle Luo |
| collection | DOAJ |
| description | Zika virus (ZIKV) infects pregnant women and causes devastating congenital zika syndrome (CZS). How the virus is vertically transmitted to the fetus and induces neuronal loss remains unclear. We previously reported that Pellino (Peli)1, an E3 ubiquitin ligase, promotes p38MAPK activation in microglia and induction of lethal encephalitis by facilitating the replication of West Nile virus (WNV), a closely related flavivirus. Here, we found that Peli1 expression was induced on ZIKV-infected human monocytic cells, peripheral blood mononuclear cells, human first-trimester placental trophoblasts, and neural stem cell (hNSC)s. Peli1 mediates ZIKV cell attachment, entry and viral translation and its expression is confined to the endoplasmic reticulum. Moreover, Peli1 mediated inflammatory cytokine and chemokine responses and induced cell death in placental trophoblasts and hNSCs. ZIKV-infected pregnant mice lacking Peli1 signaling had reduced placental inflammation and tissue damage, which resulted in attenuated congenital abnormalities. Smaducin-6, a membrane-tethered Smad6-derived peptide, blocked Peli1-mediated NF-κB activation but did not have direct effects on ZIKV infection. Smaducin-6 reduced inflammatory responses and cell death in placental trophoblasts and hNSCs, and diminished placental inflammation and damage, leading to attenuated congenital malformations in mice. Collectively, our results reveal a novel role of Peli1 in flavivirus pathogenesis and suggest that Peli1 promotes ZIKV vertical transmission and neuronal loss by mediating inflammatory cytokine responses and induction of cell death. Our results also identify Smaducin-6 as a potential therapeutic candidate for treatment of CZS. |
| first_indexed | 2024-12-21T10:24:11Z |
| format | Article |
| id | doaj.art-e6aaf087f9c54d1ebedc84ee5fae74d3 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-21T10:24:11Z |
| publishDate | 2020-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-e6aaf087f9c54d1ebedc84ee5fae74d32022-12-21T19:07:22ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742020-06-01166e100853810.1371/journal.ppat.1008538Peli1 signaling blockade attenuates congenital zika syndrome.Huanle LuoGuangyu LiBinbin WangBing TianJunling GaoJing ZouShuizhen ShiShuang ZhuBi-Hung PengAwadalkareem AdamAriza MartinezKimberly HeinEvandro R WinkelmannYoseph MahmoudXiaofei ZhouChao ShanShannan RossiScott WeaverAlan D T BarrettShao-Cong SunWenbo ZhangPei-Yong ShiPing WuTian WangZika virus (ZIKV) infects pregnant women and causes devastating congenital zika syndrome (CZS). How the virus is vertically transmitted to the fetus and induces neuronal loss remains unclear. We previously reported that Pellino (Peli)1, an E3 ubiquitin ligase, promotes p38MAPK activation in microglia and induction of lethal encephalitis by facilitating the replication of West Nile virus (WNV), a closely related flavivirus. Here, we found that Peli1 expression was induced on ZIKV-infected human monocytic cells, peripheral blood mononuclear cells, human first-trimester placental trophoblasts, and neural stem cell (hNSC)s. Peli1 mediates ZIKV cell attachment, entry and viral translation and its expression is confined to the endoplasmic reticulum. Moreover, Peli1 mediated inflammatory cytokine and chemokine responses and induced cell death in placental trophoblasts and hNSCs. ZIKV-infected pregnant mice lacking Peli1 signaling had reduced placental inflammation and tissue damage, which resulted in attenuated congenital abnormalities. Smaducin-6, a membrane-tethered Smad6-derived peptide, blocked Peli1-mediated NF-κB activation but did not have direct effects on ZIKV infection. Smaducin-6 reduced inflammatory responses and cell death in placental trophoblasts and hNSCs, and diminished placental inflammation and damage, leading to attenuated congenital malformations in mice. Collectively, our results reveal a novel role of Peli1 in flavivirus pathogenesis and suggest that Peli1 promotes ZIKV vertical transmission and neuronal loss by mediating inflammatory cytokine responses and induction of cell death. Our results also identify Smaducin-6 as a potential therapeutic candidate for treatment of CZS.https://doi.org/10.1371/journal.ppat.1008538 |
| spellingShingle | Huanle Luo Guangyu Li Binbin Wang Bing Tian Junling Gao Jing Zou Shuizhen Shi Shuang Zhu Bi-Hung Peng Awadalkareem Adam Ariza Martinez Kimberly Hein Evandro R Winkelmann Yoseph Mahmoud Xiaofei Zhou Chao Shan Shannan Rossi Scott Weaver Alan D T Barrett Shao-Cong Sun Wenbo Zhang Pei-Yong Shi Ping Wu Tian Wang Peli1 signaling blockade attenuates congenital zika syndrome. PLoS Pathogens |
| title | Peli1 signaling blockade attenuates congenital zika syndrome. |
| title_full | Peli1 signaling blockade attenuates congenital zika syndrome. |
| title_fullStr | Peli1 signaling blockade attenuates congenital zika syndrome. |
| title_full_unstemmed | Peli1 signaling blockade attenuates congenital zika syndrome. |
| title_short | Peli1 signaling blockade attenuates congenital zika syndrome. |
| title_sort | peli1 signaling blockade attenuates congenital zika syndrome |
| url | https://doi.org/10.1371/journal.ppat.1008538 |
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