Proteomic Analysis and Molecular Characterization of Airborne Bioaerosols in Indoor and Outdoor Environment in Al-Qassim Region, Saudi Arabia
There are various sources of microbial air pollution which are seems to be a serious public health problem all over the world. For prevention and control of air pollution caused by airborne bacteria, rapid, sensitive and reliable detection techniques are required. Therefore, our study focused on u...
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Journal of Pure and Applied Microbiology
2019-06-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/proteomic-analysis-and-molecular-characterization-of-airborne-bioaerosols-in-indoor-and-outdoor-environment-in-al-qassim-region-saudi-arabia/ |
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| author | Ayman Elbehiry Musaad Al-Dubaib Eman Marzouk Fahd Mohammad Albejaidi Mohamed A.A. Radwan Feras Alzaben Ahmad Alharbi |
| author_facet | Ayman Elbehiry Musaad Al-Dubaib Eman Marzouk Fahd Mohammad Albejaidi Mohamed A.A. Radwan Feras Alzaben Ahmad Alharbi |
| author_sort | Ayman Elbehiry |
| collection | DOAJ |
| description | There are various sources of microbial air pollution which are seems to be a serious public health
problem all over the world. For prevention and control of air pollution caused by airborne bacteria,
rapid, sensitive and reliable detection techniques are required. Therefore, our study focused on using
MALDI Biotyper (MBT) for rapid recognition of various microbial air pollutants. Five hundred air samples
were collected from three localities, including Qassim University (150 samples), Al-Qassim hospitals
(250 samples) and poultry slaughter houses (100 samples). All air samples were collected by impactor
air sampler from the indoor and outdoor environment. All samples were cultivated on nutrient and
blood agar media for two days and a total of 129 isolates were purified for proteomic analysis using
MALDI Biotyper (MBT) then confirmed by quantitative polymerase chain reaction (qPCR). One hundred
and nineteen (92.25%) isolates were identified by MBT at the species level with a log (score) value
≥2. 000 whereas; 10 (7.75%) isolates were detected at the genus level with score values ranged from
1.7000 to 1.999. The MBT was able to identify 93 (72.10%) gram-positive and 36 (27.90%) gram-negative
bacterial isolates. The most common genera were Staphylococcus (n = 43, 33.33%), Escherichia (n =
16, 12.40%), Enterococcus (n = 15, 11.63%) and Bacillus (n = 15, 11.63%). Staphylococcus aureus and
Escherichia coli were the most frequently identified species (n = 16, 12.40% for each). In general, we
detected 53 (41.10%) various bacterial species in Al-Qassim hospitals, 41 (31.79%) in poultry slaughter
houses and 35 (27.13%) in Qassim University. Throughout Al-Qassim region, the air was tainted by
numerous environmental microorganisms, and the MBT was positively adjusted for their fast and
accurate identification. |
| first_indexed | 2024-12-19T21:14:45Z |
| format | Article |
| id | doaj.art-e6af9881f1214aacacc1a846f60a20bd |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-19T21:14:45Z |
| publishDate | 2019-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-e6af9881f1214aacacc1a846f60a20bd2022-12-21T20:05:23ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2019-06-011321041105210.22207/JPAM.13.2.42Proteomic Analysis and Molecular Characterization of Airborne Bioaerosols in Indoor and Outdoor Environment in Al-Qassim Region, Saudi ArabiaAyman Elbehiry0Musaad Al-Dubaib1Eman Marzouk2Fahd Mohammad Albejaidi3Mohamed A.A. Radwan4Feras Alzaben5Ahmad Alharbi61 Department of Public Health, College of Public Health and Health Informatics, Qassim University, Buraidah, Saudi Arabia. 2 Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.3 Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia.1 Department of Public Health, College of Public Health and Health Informatics, Qassim University, Buraidah, Saudi Arabia.4 Department of Health Administration, College of Public Health and Health Informatics, Qassim University, Kingdom of Saudi Arabia. 5 Department of Chemistry, Faculty of Science, Qassim University, Buraidah, Saudi Arabia. 6 Applied Organic Chemistry Department, National Research Centre, Dokki, Giza, Egypt.3 Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia. 7 Department of Preventive Medicine, King Fahad Armed Forces Hospital, Jeddah City, Saudi Arabia.3 Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia. 8 Ministry of Environment Water and Agriculture, Saudi Arabia.There are various sources of microbial air pollution which are seems to be a serious public health problem all over the world. For prevention and control of air pollution caused by airborne bacteria, rapid, sensitive and reliable detection techniques are required. Therefore, our study focused on using MALDI Biotyper (MBT) for rapid recognition of various microbial air pollutants. Five hundred air samples were collected from three localities, including Qassim University (150 samples), Al-Qassim hospitals (250 samples) and poultry slaughter houses (100 samples). All air samples were collected by impactor air sampler from the indoor and outdoor environment. All samples were cultivated on nutrient and blood agar media for two days and a total of 129 isolates were purified for proteomic analysis using MALDI Biotyper (MBT) then confirmed by quantitative polymerase chain reaction (qPCR). One hundred and nineteen (92.25%) isolates were identified by MBT at the species level with a log (score) value ≥2. 000 whereas; 10 (7.75%) isolates were detected at the genus level with score values ranged from 1.7000 to 1.999. The MBT was able to identify 93 (72.10%) gram-positive and 36 (27.90%) gram-negative bacterial isolates. The most common genera were Staphylococcus (n = 43, 33.33%), Escherichia (n = 16, 12.40%), Enterococcus (n = 15, 11.63%) and Bacillus (n = 15, 11.63%). Staphylococcus aureus and Escherichia coli were the most frequently identified species (n = 16, 12.40% for each). In general, we detected 53 (41.10%) various bacterial species in Al-Qassim hospitals, 41 (31.79%) in poultry slaughter houses and 35 (27.13%) in Qassim University. Throughout Al-Qassim region, the air was tainted by numerous environmental microorganisms, and the MBT was positively adjusted for their fast and accurate identification.https://microbiologyjournal.org/proteomic-analysis-and-molecular-characterization-of-airborne-bioaerosols-in-indoor-and-outdoor-environment-in-al-qassim-region-saudi-arabia/airborne bacteriambtal-qassim regionsaudi arabia |
| spellingShingle | Ayman Elbehiry Musaad Al-Dubaib Eman Marzouk Fahd Mohammad Albejaidi Mohamed A.A. Radwan Feras Alzaben Ahmad Alharbi Proteomic Analysis and Molecular Characterization of Airborne Bioaerosols in Indoor and Outdoor Environment in Al-Qassim Region, Saudi Arabia Journal of Pure and Applied Microbiology airborne bacteria mbt al-qassim region saudi arabia |
| title | Proteomic Analysis and Molecular Characterization of Airborne Bioaerosols in Indoor and Outdoor Environment in Al-Qassim Region, Saudi Arabia |
| title_full | Proteomic Analysis and Molecular Characterization of Airborne Bioaerosols in Indoor and Outdoor Environment in Al-Qassim Region, Saudi Arabia |
| title_fullStr | Proteomic Analysis and Molecular Characterization of Airborne Bioaerosols in Indoor and Outdoor Environment in Al-Qassim Region, Saudi Arabia |
| title_full_unstemmed | Proteomic Analysis and Molecular Characterization of Airborne Bioaerosols in Indoor and Outdoor Environment in Al-Qassim Region, Saudi Arabia |
| title_short | Proteomic Analysis and Molecular Characterization of Airborne Bioaerosols in Indoor and Outdoor Environment in Al-Qassim Region, Saudi Arabia |
| title_sort | proteomic analysis and molecular characterization of airborne bioaerosols in indoor and outdoor environment in al qassim region saudi arabia |
| topic | airborne bacteria mbt al-qassim region saudi arabia |
| url | https://microbiologyjournal.org/proteomic-analysis-and-molecular-characterization-of-airborne-bioaerosols-in-indoor-and-outdoor-environment-in-al-qassim-region-saudi-arabia/ |
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