Evaluation of clonal variability of cv. Refošk (Vitis vinifera L.) by ampelographic parameters and microsatellite analysis
Twenty - one clone candidates or elites from the basic collection in the Kras winegrowing district of cv. Refošk, were studied in order to assess their genetic and morphological variability. Morphological variability was evaluated with 71 phyllometric variables or 30 less environment dependent vari...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2001-05-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/15675 |
| _version_ | 1797386946186575872 |
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| author | Petra KOZJAK Zora KOROŠEC-KORUZA Irma TOMAŽIČ Jernej JAKŠE Branka JAVORNIK |
| author_facet | Petra KOZJAK Zora KOROŠEC-KORUZA Irma TOMAŽIČ Jernej JAKŠE Branka JAVORNIK |
| author_sort | Petra KOZJAK |
| collection | DOAJ |
| description |
Twenty - one clone candidates or elites from the basic collection in the Kras winegrowing district of cv. Refošk, were studied in order to assess their genetic and morphological variability. Morphological variability was evaluated with 71 phyllometric variables or 30 less environment dependent variables, which were then used in cluster analysis. Both resultant dendrograms combined the majority of elites into closely related groups, except elite 50, which did not group with any of the analyzed elites. Microsatellites were used for the assessment of genetic variability among elites. The allelic profile at 5 microsatellite loci (VVS2, VVS4, VVMD6, VVMD7 and VVMD8) was identical for 20 elites, while elite 50 showed an unique allelic profile at all analyzed loci. Microsatellite analysis was proven to be relatively fast and simple, providing reliable results in comparison with well established ampelography. However, a combination of both methods was found useful in evaluation of variability among elites, especially when clones are phenotypically heterogenous and of unknown genetic origin, as in the case of the cv. Refošk basic collection.
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| first_indexed | 2024-03-08T22:16:46Z |
| format | Article |
| id | doaj.art-e6afbef4c7314d12b7f527e39262f3f0 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-08T22:16:46Z |
| publishDate | 2001-05-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-e6afbef4c7314d12b7f527e39262f3f02023-12-18T22:15:03ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412001-05-01771Evaluation of clonal variability of cv. Refošk (Vitis vinifera L.) by ampelographic parameters and microsatellite analysisPetra KOZJAK0Zora KOROŠEC-KORUZA1Irma TOMAŽIČJernej JAKŠEBranka JAVORNIK2Agricultural Institute of Slovenia, Hacquetova 17, SI-1000 LjubljanaUniversity of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 LjubljanaUniversity of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana Twenty - one clone candidates or elites from the basic collection in the Kras winegrowing district of cv. Refošk, were studied in order to assess their genetic and morphological variability. Morphological variability was evaluated with 71 phyllometric variables or 30 less environment dependent variables, which were then used in cluster analysis. Both resultant dendrograms combined the majority of elites into closely related groups, except elite 50, which did not group with any of the analyzed elites. Microsatellites were used for the assessment of genetic variability among elites. The allelic profile at 5 microsatellite loci (VVS2, VVS4, VVMD6, VVMD7 and VVMD8) was identical for 20 elites, while elite 50 showed an unique allelic profile at all analyzed loci. Microsatellite analysis was proven to be relatively fast and simple, providing reliable results in comparison with well established ampelography. However, a combination of both methods was found useful in evaluation of variability among elites, especially when clones are phenotypically heterogenous and of unknown genetic origin, as in the case of the cv. Refošk basic collection. https://journals.uni-lj.si/aas/article/view/15675Vitis viniferacv. Refoškampelographyelitemicrosatellitevariability |
| spellingShingle | Petra KOZJAK Zora KOROŠEC-KORUZA Irma TOMAŽIČ Jernej JAKŠE Branka JAVORNIK Evaluation of clonal variability of cv. Refošk (Vitis vinifera L.) by ampelographic parameters and microsatellite analysis Acta Agriculturae Slovenica Vitis vinifera cv. Refošk ampelography elite microsatellite variability |
| title | Evaluation of clonal variability of cv. Refošk (Vitis vinifera L.) by ampelographic parameters and microsatellite analysis |
| title_full | Evaluation of clonal variability of cv. Refošk (Vitis vinifera L.) by ampelographic parameters and microsatellite analysis |
| title_fullStr | Evaluation of clonal variability of cv. Refošk (Vitis vinifera L.) by ampelographic parameters and microsatellite analysis |
| title_full_unstemmed | Evaluation of clonal variability of cv. Refošk (Vitis vinifera L.) by ampelographic parameters and microsatellite analysis |
| title_short | Evaluation of clonal variability of cv. Refošk (Vitis vinifera L.) by ampelographic parameters and microsatellite analysis |
| title_sort | evaluation of clonal variability of cv refosk vitis vinifera l by ampelographic parameters and microsatellite analysis |
| topic | Vitis vinifera cv. Refošk ampelography elite microsatellite variability |
| url | https://journals.uni-lj.si/aas/article/view/15675 |
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