Biotransformation of se-methylselenocysteine into volatile selenocompounds by bacteria isolated from rat gut microflora
Selenium (Se) is an essential micronutrient that exists in various chemical forms in nature. Because of this property, animals must metabolize diverse Se species to utilize them as selenoproteins. Although gut microflora is suggested to play a role in Se metabolism in host animal, the biotransformat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623004590 |
| _version_ | 1797632733148610560 |
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| author | Kazuaki Takahashi Sakie Horiai Yoshikazu Yamagishi Sayaka Nagasawa Hirotaro Iwase Yasumitsu Ogra |
| author_facet | Kazuaki Takahashi Sakie Horiai Yoshikazu Yamagishi Sayaka Nagasawa Hirotaro Iwase Yasumitsu Ogra |
| author_sort | Kazuaki Takahashi |
| collection | DOAJ |
| description | Selenium (Se) is an essential micronutrient that exists in various chemical forms in nature. Because of this property, animals must metabolize diverse Se species to utilize them as selenoproteins. Although gut microflora is suggested to play a role in Se metabolism in host animal, the biotransformation of Se by gut microflora in animal gut is not fully understood. In this study, we isolated Morganella morganii from rat feces under the condition that Se-methylseleno-L-cysteine (MeSeCys), one of the major Se sources in vegetables, was present in excess. Then, we examined the biotransformation of MeSeCys by M. morganii. Two volatile selenocompounds, dimethyldiselenide (DMDSe) and dimethylselenide (DMSe), were detected by GC–MS in the headspace of M. morganii culture. We speculate that M. morganii cleaved off the methylselenyl group from MeSeCys. Taken together, we conclude that gut microflora plays a role in the biotransformation of MeSeCys, and seems to support efficient metabolism in animals. |
| first_indexed | 2024-03-11T11:41:59Z |
| format | Article |
| id | doaj.art-e6b2d6cf6b7e4b5a997734eaa24bfb4e |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-11T11:41:59Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e6b2d6cf6b7e4b5a997734eaa24bfb4e2023-11-10T04:16:14ZengElsevierJournal of Functional Foods1756-46462023-11-01110105859Biotransformation of se-methylselenocysteine into volatile selenocompounds by bacteria isolated from rat gut microfloraKazuaki Takahashi0Sakie Horiai1Yoshikazu Yamagishi2Sayaka Nagasawa3Hirotaro Iwase4Yasumitsu Ogra5Graduate School of Horticulture, Chiba University, 1-33 Yayoi Cho, Inage, Chiba 263-8522, JapanGraduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, JapanGraduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8670, JapanGraduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8670, JapanGraduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8670, JapanGraduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, Japan; Corresponding author at: Laboratory of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, Japan.Selenium (Se) is an essential micronutrient that exists in various chemical forms in nature. Because of this property, animals must metabolize diverse Se species to utilize them as selenoproteins. Although gut microflora is suggested to play a role in Se metabolism in host animal, the biotransformation of Se by gut microflora in animal gut is not fully understood. In this study, we isolated Morganella morganii from rat feces under the condition that Se-methylseleno-L-cysteine (MeSeCys), one of the major Se sources in vegetables, was present in excess. Then, we examined the biotransformation of MeSeCys by M. morganii. Two volatile selenocompounds, dimethyldiselenide (DMDSe) and dimethylselenide (DMSe), were detected by GC–MS in the headspace of M. morganii culture. We speculate that M. morganii cleaved off the methylselenyl group from MeSeCys. Taken together, we conclude that gut microflora plays a role in the biotransformation of MeSeCys, and seems to support efficient metabolism in animals.http://www.sciencedirect.com/science/article/pii/S1756464623004590SeleniumDimethyldiselenideDimethylselenideM. morganiiGC–MSGut microflora |
| spellingShingle | Kazuaki Takahashi Sakie Horiai Yoshikazu Yamagishi Sayaka Nagasawa Hirotaro Iwase Yasumitsu Ogra Biotransformation of se-methylselenocysteine into volatile selenocompounds by bacteria isolated from rat gut microflora Journal of Functional Foods Selenium Dimethyldiselenide Dimethylselenide M. morganii GC–MS Gut microflora |
| title | Biotransformation of se-methylselenocysteine into volatile selenocompounds by bacteria isolated from rat gut microflora |
| title_full | Biotransformation of se-methylselenocysteine into volatile selenocompounds by bacteria isolated from rat gut microflora |
| title_fullStr | Biotransformation of se-methylselenocysteine into volatile selenocompounds by bacteria isolated from rat gut microflora |
| title_full_unstemmed | Biotransformation of se-methylselenocysteine into volatile selenocompounds by bacteria isolated from rat gut microflora |
| title_short | Biotransformation of se-methylselenocysteine into volatile selenocompounds by bacteria isolated from rat gut microflora |
| title_sort | biotransformation of se methylselenocysteine into volatile selenocompounds by bacteria isolated from rat gut microflora |
| topic | Selenium Dimethyldiselenide Dimethylselenide M. morganii GC–MS Gut microflora |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623004590 |
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