Polymorphism of Myostatin Gene and its Association with Body Weight of Iraqi Local Ducks

Myostatin, or growth and differentiation factor-8(GDF-8) is a member of the transforming growth factor (TGF)-ß superfamily. The objective of this study was to investigate the association between genetic polymorphism of the myostatin gene with the body-weight of Iraqi local ducks by DNA sequence anal...

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Main Authors: Ali. J. Ghali, Sajida A. Al-shaheen
Format: Article
Language:English
Published: University of Basrah 2019-11-01
Series:Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ
Subjects:
Online Access:https://bjas.bajas.edu.iq/index.php/bjas/article/view/145
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author Ali. J. Ghali
Sajida A. Al-shaheen
author_facet Ali. J. Ghali
Sajida A. Al-shaheen
author_sort Ali. J. Ghali
collection DOAJ
description Myostatin, or growth and differentiation factor-8(GDF-8) is a member of the transforming growth factor (TGF)-ß superfamily. The objective of this study was to investigate the association between genetic polymorphism of the myostatin gene with the body-weight of Iraqi local ducks by DNA sequence analysis. The results of DNA sequence showed nitrogen base change (G?A) at 129 base pair sites of the exon1 of MSTN gene. Therefore, two alleles, G and A and two patterns of genotypes, GG, AG were observed. The observed numbers of GG and AG genotypes were 25 and 6, respectively. The G allele was of the highest frequency (0.9) while A allele showed the lowest frequency (0.1). The GG genotype showed the highest frequency (80.65%) and AG genotype showed the lowest frequency (19.35%). Additionally, the percentages of observed and expected heterozygosity were 0.19 and 0.17 respectively. Moreover, the percentages of observed and expected homozygosity were 0.80 and 0.82, respectively. The average heterozygosity was 0.17. The results revealed no significant (P?0.05) effect of the MSTN gene on body weight.
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spelling doaj.art-e6b65e38c50f4ec98268353bba6757f42022-12-21T19:32:30ZengUniversity of BasrahMaǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ1814-58682520-08602019-11-013210.37077/25200860.2019.260Polymorphism of Myostatin Gene and its Association with Body Weight of Iraqi Local DucksAli. J. Ghali0Sajida A. Al-shaheen1Department of Animal Production, College of Agriculture, University of BasrahDepartment of Animal Production, College of Agriculture, University of BasrahMyostatin, or growth and differentiation factor-8(GDF-8) is a member of the transforming growth factor (TGF)-ß superfamily. The objective of this study was to investigate the association between genetic polymorphism of the myostatin gene with the body-weight of Iraqi local ducks by DNA sequence analysis. The results of DNA sequence showed nitrogen base change (G?A) at 129 base pair sites of the exon1 of MSTN gene. Therefore, two alleles, G and A and two patterns of genotypes, GG, AG were observed. The observed numbers of GG and AG genotypes were 25 and 6, respectively. The G allele was of the highest frequency (0.9) while A allele showed the lowest frequency (0.1). The GG genotype showed the highest frequency (80.65%) and AG genotype showed the lowest frequency (19.35%). Additionally, the percentages of observed and expected heterozygosity were 0.19 and 0.17 respectively. Moreover, the percentages of observed and expected homozygosity were 0.80 and 0.82, respectively. The average heterozygosity was 0.17. The results revealed no significant (P?0.05) effect of the MSTN gene on body weight. https://bjas.bajas.edu.iq/index.php/bjas/article/view/145Myostatin geneIraqi local duckspolymorphismDNA sequence analysis
spellingShingle Ali. J. Ghali
Sajida A. Al-shaheen
Polymorphism of Myostatin Gene and its Association with Body Weight of Iraqi Local Ducks
Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ
Myostatin gene
Iraqi local ducks
polymorphism
DNA sequence analysis
title Polymorphism of Myostatin Gene and its Association with Body Weight of Iraqi Local Ducks
title_full Polymorphism of Myostatin Gene and its Association with Body Weight of Iraqi Local Ducks
title_fullStr Polymorphism of Myostatin Gene and its Association with Body Weight of Iraqi Local Ducks
title_full_unstemmed Polymorphism of Myostatin Gene and its Association with Body Weight of Iraqi Local Ducks
title_short Polymorphism of Myostatin Gene and its Association with Body Weight of Iraqi Local Ducks
title_sort polymorphism of myostatin gene and its association with body weight of iraqi local ducks
topic Myostatin gene
Iraqi local ducks
polymorphism
DNA sequence analysis
url https://bjas.bajas.edu.iq/index.php/bjas/article/view/145
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AT sajidaaalshaheen polymorphismofmyostatingeneanditsassociationwithbodyweightofiraqilocalducks