Evaluation of antioxidant and cytotoxic activity of herbal teas from Western Himalayan region: a comparison with green tea (Camellia sinensis) and black tea
Abstract Background Herbal tea, known as health-promoting due to its therapeutic potential for several ailments and consumption increased over decades. The Western Himalayan region of India affluent with herbs has therapeutic values. However, these herbs have not been used in the tea and are still u...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-05-01
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| Series: | Chemical and Biological Technologies in Agriculture |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40538-022-00294-3 |
| _version_ | 1811252216441339904 |
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| author | Amita Kumari Dharmesh Kumar |
| author_facet | Amita Kumari Dharmesh Kumar |
| author_sort | Amita Kumari |
| collection | DOAJ |
| description | Abstract Background Herbal tea, known as health-promoting due to its therapeutic potential for several ailments and consumption increased over decades. The Western Himalayan region of India affluent with herbs has therapeutic values. However, these herbs have not been used in the tea and are still untouched by mankind. Therefore, the present study aimed to pioneer and manufacture herbal teas from Western Himalayan region plants. Results Our findings showed that total polyphenol content was ranged from 4.42 ± 0.53 to 13.37 ± 0.50% compared to green tea (GT) and black tea (BT) (13.37 ± 0.50 and 10.05 ± 0.11%) of C. sinensis, total flavonoid and total catechin content was ranged from 1.81 ± 0.67–4.68 ± 0.26% to 4.43 ± 0.28–15.17 ± 0.53% in all the herbal tea samples. Moreover, antioxidant activity was ranged in DPPH from (27.58–226.28 µg/mL) and in ABTS (14.17–117.62 µg/mL). Highest antioxidant activity was observed in GT and lowest was observed in rose tea (RT). Heatmap was made for catechin visualization in green herbal teas (GHT). Principal Component Analysis (PCA) showed the variation of amino acids in all the herbal tea samples which was found in the range from 0.82 to 2.86%. Taxus green tea (TGT) exhibited remarkable cytotoxic activity against SW480 (50.9 ± 0.7 at 200 µg/mL). Whereas, sea-buckthorn green tea (SGT) exhibited the highest activities on A549 cells (87.01 ± 1.1 at 200 µg/mL). Maximum volatile organic compounds (VOCs) were identified in lemongrass black tea (LBT) (96.23%), namely, geranial, levoverbenone, pulegone, l-linalool and cineol. In addition, the sensory analysis revealed that herbal tea shows sweet and better taste with high sensory attributes. Conclusions Current study revealed that the Western Himalayan region plants could be used as herbal tea with additional health benefits. The prepared herbal teas can be used in nutraceuticals as a beverage and a new dietary source for bioactive compounds. Graphical Abstract |
| first_indexed | 2024-04-12T16:31:33Z |
| format | Article |
| id | doaj.art-e6b6afb3387f4ed39f4eab6026f1fa43 |
| institution | Directory Open Access Journal |
| issn | 2196-5641 |
| language | English |
| last_indexed | 2024-04-12T16:31:33Z |
| publishDate | 2022-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj.art-e6b6afb3387f4ed39f4eab6026f1fa432022-12-22T03:25:08ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412022-05-019112010.1186/s40538-022-00294-3Evaluation of antioxidant and cytotoxic activity of herbal teas from Western Himalayan region: a comparison with green tea (Camellia sinensis) and black teaAmita Kumari0Dharmesh Kumar1Chemical Technology Division, CSIR - Institute of Himalayan Bioresource TechnologyDeptartment of Sophisticated Analytical Instrument Facility and Research, CSIR - Central Drug Research InstituteAbstract Background Herbal tea, known as health-promoting due to its therapeutic potential for several ailments and consumption increased over decades. The Western Himalayan region of India affluent with herbs has therapeutic values. However, these herbs have not been used in the tea and are still untouched by mankind. Therefore, the present study aimed to pioneer and manufacture herbal teas from Western Himalayan region plants. Results Our findings showed that total polyphenol content was ranged from 4.42 ± 0.53 to 13.37 ± 0.50% compared to green tea (GT) and black tea (BT) (13.37 ± 0.50 and 10.05 ± 0.11%) of C. sinensis, total flavonoid and total catechin content was ranged from 1.81 ± 0.67–4.68 ± 0.26% to 4.43 ± 0.28–15.17 ± 0.53% in all the herbal tea samples. Moreover, antioxidant activity was ranged in DPPH from (27.58–226.28 µg/mL) and in ABTS (14.17–117.62 µg/mL). Highest antioxidant activity was observed in GT and lowest was observed in rose tea (RT). Heatmap was made for catechin visualization in green herbal teas (GHT). Principal Component Analysis (PCA) showed the variation of amino acids in all the herbal tea samples which was found in the range from 0.82 to 2.86%. Taxus green tea (TGT) exhibited remarkable cytotoxic activity against SW480 (50.9 ± 0.7 at 200 µg/mL). Whereas, sea-buckthorn green tea (SGT) exhibited the highest activities on A549 cells (87.01 ± 1.1 at 200 µg/mL). Maximum volatile organic compounds (VOCs) were identified in lemongrass black tea (LBT) (96.23%), namely, geranial, levoverbenone, pulegone, l-linalool and cineol. In addition, the sensory analysis revealed that herbal tea shows sweet and better taste with high sensory attributes. Conclusions Current study revealed that the Western Himalayan region plants could be used as herbal tea with additional health benefits. The prepared herbal teas can be used in nutraceuticals as a beverage and a new dietary source for bioactive compounds. Graphical Abstracthttps://doi.org/10.1186/s40538-022-00294-3Herbal teasCamellia sinensisAntioxidant activityCytotoxic activityCatechins |
| spellingShingle | Amita Kumari Dharmesh Kumar Evaluation of antioxidant and cytotoxic activity of herbal teas from Western Himalayan region: a comparison with green tea (Camellia sinensis) and black tea Chemical and Biological Technologies in Agriculture Herbal teas Camellia sinensis Antioxidant activity Cytotoxic activity Catechins |
| title | Evaluation of antioxidant and cytotoxic activity of herbal teas from Western Himalayan region: a comparison with green tea (Camellia sinensis) and black tea |
| title_full | Evaluation of antioxidant and cytotoxic activity of herbal teas from Western Himalayan region: a comparison with green tea (Camellia sinensis) and black tea |
| title_fullStr | Evaluation of antioxidant and cytotoxic activity of herbal teas from Western Himalayan region: a comparison with green tea (Camellia sinensis) and black tea |
| title_full_unstemmed | Evaluation of antioxidant and cytotoxic activity of herbal teas from Western Himalayan region: a comparison with green tea (Camellia sinensis) and black tea |
| title_short | Evaluation of antioxidant and cytotoxic activity of herbal teas from Western Himalayan region: a comparison with green tea (Camellia sinensis) and black tea |
| title_sort | evaluation of antioxidant and cytotoxic activity of herbal teas from western himalayan region a comparison with green tea camellia sinensis and black tea |
| topic | Herbal teas Camellia sinensis Antioxidant activity Cytotoxic activity Catechins |
| url | https://doi.org/10.1186/s40538-022-00294-3 |
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