Gelatin Blends Enhance Performance of Electrospun Polymeric Scaffolds in Comparison to Coating Protocols
The electrospinning of hybrid polymers is a versatile fabrication technique which takes advantage of the biological properties of natural polymers and the mechanical properties of synthetic polymers. However, the literature is scarce when it comes to comparisons of blends regarding coatings and the...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/14/7/1311 |
| _version_ | 1797438014622793728 |
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| author | Maria Bikuna-Izagirre Javier Aldazabal Jacobo Paredes |
| author_facet | Maria Bikuna-Izagirre Javier Aldazabal Jacobo Paredes |
| author_sort | Maria Bikuna-Izagirre |
| collection | DOAJ |
| description | The electrospinning of hybrid polymers is a versatile fabrication technique which takes advantage of the biological properties of natural polymers and the mechanical properties of synthetic polymers. However, the literature is scarce when it comes to comparisons of blends regarding coatings and the improvements offered thereby in terms of cellular performance. To address this, in the present study, nanofibrous electrospun scaffolds of polycaprolactone (PCL), their coating and their blend with gelatin were compared. The morphology of nanofibrous scaffolds was analyzed under field emission scanning electron microscopy (FE-SEM), indicating the influence of the presence of gelatin. The scaffolds were mechanically tested with tensile tests; PCL and PCL gelatin coated scaffolds showed higher elastic moduli than PCL/gelatin meshes. Viability of mouse embryonic fibroblasts (MEF) was evaluated by MTT assay, and cell proliferation on the scaffold was confirmed by fluorescence staining. The positive results of the MTT assay and cell growth indicated that the scaffolds of PCL/gelatin excelled in comparison to other scaffolds, and may serve as good candidates for tissue engineering applications. |
| first_indexed | 2024-03-09T11:31:00Z |
| format | Article |
| id | doaj.art-e6b8c2e2d90540a1aca4ace35b7beddc |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T11:31:00Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-e6b8c2e2d90540a1aca4ace35b7beddc2023-11-30T23:52:43ZengMDPI AGPolymers2073-43602022-03-01147131110.3390/polym14071311Gelatin Blends Enhance Performance of Electrospun Polymeric Scaffolds in Comparison to Coating ProtocolsMaria Bikuna-Izagirre0Javier Aldazabal1Jacobo Paredes2Tecnun School of Engineering, University of Navarra, Manuel Lardizabal 13, 20018 San Sebastian, SpainTecnun School of Engineering, University of Navarra, Manuel Lardizabal 13, 20018 San Sebastian, SpainTecnun School of Engineering, University of Navarra, Manuel Lardizabal 13, 20018 San Sebastian, SpainThe electrospinning of hybrid polymers is a versatile fabrication technique which takes advantage of the biological properties of natural polymers and the mechanical properties of synthetic polymers. However, the literature is scarce when it comes to comparisons of blends regarding coatings and the improvements offered thereby in terms of cellular performance. To address this, in the present study, nanofibrous electrospun scaffolds of polycaprolactone (PCL), their coating and their blend with gelatin were compared. The morphology of nanofibrous scaffolds was analyzed under field emission scanning electron microscopy (FE-SEM), indicating the influence of the presence of gelatin. The scaffolds were mechanically tested with tensile tests; PCL and PCL gelatin coated scaffolds showed higher elastic moduli than PCL/gelatin meshes. Viability of mouse embryonic fibroblasts (MEF) was evaluated by MTT assay, and cell proliferation on the scaffold was confirmed by fluorescence staining. The positive results of the MTT assay and cell growth indicated that the scaffolds of PCL/gelatin excelled in comparison to other scaffolds, and may serve as good candidates for tissue engineering applications.https://www.mdpi.com/2073-4360/14/7/1311electrospinningscaffoldPCLgelatintissue engineeringmechanical properties |
| spellingShingle | Maria Bikuna-Izagirre Javier Aldazabal Jacobo Paredes Gelatin Blends Enhance Performance of Electrospun Polymeric Scaffolds in Comparison to Coating Protocols Polymers electrospinning scaffold PCL gelatin tissue engineering mechanical properties |
| title | Gelatin Blends Enhance Performance of Electrospun Polymeric Scaffolds in Comparison to Coating Protocols |
| title_full | Gelatin Blends Enhance Performance of Electrospun Polymeric Scaffolds in Comparison to Coating Protocols |
| title_fullStr | Gelatin Blends Enhance Performance of Electrospun Polymeric Scaffolds in Comparison to Coating Protocols |
| title_full_unstemmed | Gelatin Blends Enhance Performance of Electrospun Polymeric Scaffolds in Comparison to Coating Protocols |
| title_short | Gelatin Blends Enhance Performance of Electrospun Polymeric Scaffolds in Comparison to Coating Protocols |
| title_sort | gelatin blends enhance performance of electrospun polymeric scaffolds in comparison to coating protocols |
| topic | electrospinning scaffold PCL gelatin tissue engineering mechanical properties |
| url | https://www.mdpi.com/2073-4360/14/7/1311 |
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