Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions
Although foxtail millet, as small Panicoid crop, is of drought resilient, drought stress has a significant effect on panicle of foxtail millet at the yield formation stage. In this study, the changes of panicle morphology, photosynthesis, antioxidant protective enzyme system, reactive oxygen species...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1110910/full |
| _version_ | 1811172908446253056 |
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| author | Jing Wang Zexin Sun Xinning Wang Ying Tang Xinyi Li Chuanyou Ren Jingyao Ren Xiaoguang Wang Chunji Jiang Chao Zhong Shuli Zhao He Zhang Xibo Liu Shuli Kang Xinhua Zhao Haiqiu Yu |
| author_facet | Jing Wang Zexin Sun Xinning Wang Ying Tang Xinyi Li Chuanyou Ren Jingyao Ren Xiaoguang Wang Chunji Jiang Chao Zhong Shuli Zhao He Zhang Xibo Liu Shuli Kang Xinhua Zhao Haiqiu Yu |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Although foxtail millet, as small Panicoid crop, is of drought resilient, drought stress has a significant effect on panicle of foxtail millet at the yield formation stage. In this study, the changes of panicle morphology, photosynthesis, antioxidant protective enzyme system, reactive oxygen species (ROS) system, and osmotic regulatory substance and RNA-seq of functional leaves under light drought stress (LD), heavy drought stress (HD), light drought control (LDCK) and heavy drought control (HDCK) were studied to get a snap-shot of specific panicle morphological changes, physiological responses and related molecular mechanisms. The results showed that the length and weight of panicle had decreased, but with increased empty abortive rate, and then yield dropped off 14.9% and 36.9%, respectively. The photosynthesis of millet was significantly decreased, like net photosynthesis rate, stomatal conductance and transpiration rate, especially under HD treatment with reluctant recovery from rehydration. Under LD and HD treatment, the peroxidase (POD) was increased by 34% and 14% and the same as H2O2 by 34.7% and 17.2% compared with LDCK and HDCK. The ability to produce and inhibit O2- free radicals under LD treatment was higher than HD. The content of soluble sugar was higher under LD treatment but the proline was higher under HD treatment. Through RNA-seq analysis, there were 2,393 and 3,078 different genes expressed under LD and HD treatment. According to the correlation analysis between weighted gene coexpression network analysis (WGCNA) and physiological traits, the co-expression network of several modules with high correlation was constructed, and some hub genes of millet in response to drought stress were found. The expression changes relating to carbon fixation, sucrose and starch synthesis, lignin synthesis, gibberellin synthesis, and proline synthesis of millet were specifically analyzed. These findings provide a full perspective on how drought affects the yield formation of foxtail millet by constructing one work model thereby providing theoretical foundation for hub genes exploration and drought resistance breeding of foxtail millet. |
| first_indexed | 2024-04-10T17:38:21Z |
| format | Article |
| id | doaj.art-e6b8d749f0d24df1940c684c65060f9a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T17:38:21Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e6b8d749f0d24df1940c684c65060f9a2023-02-03T16:34:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011310.3389/fpls.2022.11109101110910Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditionsJing WangZexin SunXinning WangYing TangXinyi LiChuanyou RenJingyao RenXiaoguang WangChunji JiangChao ZhongShuli ZhaoHe ZhangXibo LiuShuli KangXinhua ZhaoHaiqiu YuAlthough foxtail millet, as small Panicoid crop, is of drought resilient, drought stress has a significant effect on panicle of foxtail millet at the yield formation stage. In this study, the changes of panicle morphology, photosynthesis, antioxidant protective enzyme system, reactive oxygen species (ROS) system, and osmotic regulatory substance and RNA-seq of functional leaves under light drought stress (LD), heavy drought stress (HD), light drought control (LDCK) and heavy drought control (HDCK) were studied to get a snap-shot of specific panicle morphological changes, physiological responses and related molecular mechanisms. The results showed that the length and weight of panicle had decreased, but with increased empty abortive rate, and then yield dropped off 14.9% and 36.9%, respectively. The photosynthesis of millet was significantly decreased, like net photosynthesis rate, stomatal conductance and transpiration rate, especially under HD treatment with reluctant recovery from rehydration. Under LD and HD treatment, the peroxidase (POD) was increased by 34% and 14% and the same as H2O2 by 34.7% and 17.2% compared with LDCK and HDCK. The ability to produce and inhibit O2- free radicals under LD treatment was higher than HD. The content of soluble sugar was higher under LD treatment but the proline was higher under HD treatment. Through RNA-seq analysis, there were 2,393 and 3,078 different genes expressed under LD and HD treatment. According to the correlation analysis between weighted gene coexpression network analysis (WGCNA) and physiological traits, the co-expression network of several modules with high correlation was constructed, and some hub genes of millet in response to drought stress were found. The expression changes relating to carbon fixation, sucrose and starch synthesis, lignin synthesis, gibberellin synthesis, and proline synthesis of millet were specifically analyzed. These findings provide a full perspective on how drought affects the yield formation of foxtail millet by constructing one work model thereby providing theoretical foundation for hub genes exploration and drought resistance breeding of foxtail millet.https://www.frontiersin.org/articles/10.3389/fpls.2022.1110910/fullfoxtail milletdrought stressphysiological indexRNA-seqDEGsyield formation |
| spellingShingle | Jing Wang Zexin Sun Xinning Wang Ying Tang Xinyi Li Chuanyou Ren Jingyao Ren Xiaoguang Wang Chunji Jiang Chao Zhong Shuli Zhao He Zhang Xibo Liu Shuli Kang Xinhua Zhao Haiqiu Yu Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions Frontiers in Plant Science foxtail millet drought stress physiological index RNA-seq DEGs yield formation |
| title | Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions |
| title_full | Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions |
| title_fullStr | Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions |
| title_full_unstemmed | Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions |
| title_short | Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions |
| title_sort | transcriptome based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions |
| topic | foxtail millet drought stress physiological index RNA-seq DEGs yield formation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1110910/full |
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