Endophytic Bacteria Associated with <i>Origanum</i> <i>heracleoticum</i> L. (Lamiaceae) Seeds
Seed-associated microbiota are believed to play a crucial role in seed germination, seedling establishment, and plant growth and fitness stimulation, due to the vertical transmission of a core microbiota from seeds to the next generations. It might be hypothesized that medicinal and aromatic plants...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/10/2086 |
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| author | Giulia Semenzato Teresa Faddetta Sara Falsini Sara Del Duca Antonia Esposito Anna Padula Claudia Greco Nadia Mucci Marco Zaccaroni Anna Maria Puglia Alessio Papini Renato Fani |
| author_facet | Giulia Semenzato Teresa Faddetta Sara Falsini Sara Del Duca Antonia Esposito Anna Padula Claudia Greco Nadia Mucci Marco Zaccaroni Anna Maria Puglia Alessio Papini Renato Fani |
| author_sort | Giulia Semenzato |
| collection | DOAJ |
| description | Seed-associated microbiota are believed to play a crucial role in seed germination, seedling establishment, and plant growth and fitness stimulation, due to the vertical transmission of a core microbiota from seeds to the next generations. It might be hypothesized that medicinal and aromatic plants could use the seeds as vectors to vertically transfer beneficial endophytes, providing plants with metabolic pathways that could influence phytochemicals production. Here, we investigated the localization, the structure and the composition of the bacterial endophytic population that resides in <i>Origanum heracleoticum</i> L. seeds. Endocellular bacteria, surrounded by a wall, were localized close to the aleurone layer when using light and transmission electron microscopy. From surface-sterilized seeds, cultivable endophytes were isolated and characterized through RAPD analysis and 16S RNA gene sequencing, which revealed the existence of a high degree of biodiversity at the strain level and the predominance of the genus <i>Pseudomonas</i>. Most of the isolates grew in the presence of six selected antibiotics and were able to inhibit the growth of clinical and environmental strains that belong to the <i>Burkholderia cepacia</i> complex. The endophytes production of antimicrobial compounds could suggest their involvement in plant secondary metabolites production and might pave the way to endophytes exploitation in the pharmaceutical field. |
| first_indexed | 2024-03-09T19:44:52Z |
| format | Article |
| id | doaj.art-e6bebd52e3ca4b3ca726a0e2fdbfd8f3 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T19:44:52Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e6bebd52e3ca4b3ca726a0e2fdbfd8f32023-11-24T01:28:10ZengMDPI AGMicroorganisms2076-26072022-10-011010208610.3390/microorganisms10102086Endophytic Bacteria Associated with <i>Origanum</i> <i>heracleoticum</i> L. (Lamiaceae) SeedsGiulia Semenzato0Teresa Faddetta1Sara Falsini2Sara Del Duca3Antonia Esposito4Anna Padula5Claudia Greco6Nadia Mucci7Marco Zaccaroni8Anna Maria Puglia9Alessio Papini10Renato Fani11Department of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies-STEBICEF, University of Palermo, Viale delle Scienze Ed. 17, 90128 Palermo, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyUnit for Conservation Genetics (BIO-CGE), Institute for Environmental Protection and Research, Via Ca’ Fornacetta, 9, Ozzano dell’Emilia, 40064 Bologna, ItalyUnit for Conservation Genetics (BIO-CGE), Institute for Environmental Protection and Research, Via Ca’ Fornacetta, 9, Ozzano dell’Emilia, 40064 Bologna, ItalyUnit for Conservation Genetics (BIO-CGE), Institute for Environmental Protection and Research, Via Ca’ Fornacetta, 9, Ozzano dell’Emilia, 40064 Bologna, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies-STEBICEF, University of Palermo, Viale delle Scienze Ed. 17, 90128 Palermo, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalySeed-associated microbiota are believed to play a crucial role in seed germination, seedling establishment, and plant growth and fitness stimulation, due to the vertical transmission of a core microbiota from seeds to the next generations. It might be hypothesized that medicinal and aromatic plants could use the seeds as vectors to vertically transfer beneficial endophytes, providing plants with metabolic pathways that could influence phytochemicals production. Here, we investigated the localization, the structure and the composition of the bacterial endophytic population that resides in <i>Origanum heracleoticum</i> L. seeds. Endocellular bacteria, surrounded by a wall, were localized close to the aleurone layer when using light and transmission electron microscopy. From surface-sterilized seeds, cultivable endophytes were isolated and characterized through RAPD analysis and 16S RNA gene sequencing, which revealed the existence of a high degree of biodiversity at the strain level and the predominance of the genus <i>Pseudomonas</i>. Most of the isolates grew in the presence of six selected antibiotics and were able to inhibit the growth of clinical and environmental strains that belong to the <i>Burkholderia cepacia</i> complex. The endophytes production of antimicrobial compounds could suggest their involvement in plant secondary metabolites production and might pave the way to endophytes exploitation in the pharmaceutical field.https://www.mdpi.com/2076-2607/10/10/2086medicinal plantsmicrobiomeseed-associated endophytesessential oilantimicrobial compoundsphytobiome |
| spellingShingle | Giulia Semenzato Teresa Faddetta Sara Falsini Sara Del Duca Antonia Esposito Anna Padula Claudia Greco Nadia Mucci Marco Zaccaroni Anna Maria Puglia Alessio Papini Renato Fani Endophytic Bacteria Associated with <i>Origanum</i> <i>heracleoticum</i> L. (Lamiaceae) Seeds Microorganisms medicinal plants microbiome seed-associated endophytes essential oil antimicrobial compounds phytobiome |
| title | Endophytic Bacteria Associated with <i>Origanum</i> <i>heracleoticum</i> L. (Lamiaceae) Seeds |
| title_full | Endophytic Bacteria Associated with <i>Origanum</i> <i>heracleoticum</i> L. (Lamiaceae) Seeds |
| title_fullStr | Endophytic Bacteria Associated with <i>Origanum</i> <i>heracleoticum</i> L. (Lamiaceae) Seeds |
| title_full_unstemmed | Endophytic Bacteria Associated with <i>Origanum</i> <i>heracleoticum</i> L. (Lamiaceae) Seeds |
| title_short | Endophytic Bacteria Associated with <i>Origanum</i> <i>heracleoticum</i> L. (Lamiaceae) Seeds |
| title_sort | endophytic bacteria associated with i origanum i i heracleoticum i l lamiaceae seeds |
| topic | medicinal plants microbiome seed-associated endophytes essential oil antimicrobial compounds phytobiome |
| url | https://www.mdpi.com/2076-2607/10/10/2086 |
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