Cytomegalovirus-Specific T Cells in Pediatric Liver Transplant Recipients
Cytomegalovirus (CMV) infection is a major opportunistic infection after liver transplantation (LT) that necessitates monitoring. Because of the lack of studies in children, we aimed to investigate CMV-specific T cell immune reconstitution among pediatric LT recipients. The recipients were monitored...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/11/2213 |
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| author | Songpon Getsuwan Nopporn Apiwattanakul Chatmanee Lertudomphonwanit Suradej Hongeng Sophida Boonsathorn Wiparat Manuyakorn Pornthep Tanpowpong Usanarat Anurathapan Kanchana Tangnararatchakit Suporn Treepongkaruna |
| author_facet | Songpon Getsuwan Nopporn Apiwattanakul Chatmanee Lertudomphonwanit Suradej Hongeng Sophida Boonsathorn Wiparat Manuyakorn Pornthep Tanpowpong Usanarat Anurathapan Kanchana Tangnararatchakit Suporn Treepongkaruna |
| author_sort | Songpon Getsuwan |
| collection | DOAJ |
| description | Cytomegalovirus (CMV) infection is a major opportunistic infection after liver transplantation (LT) that necessitates monitoring. Because of the lack of studies in children, we aimed to investigate CMV-specific T cell immune reconstitution among pediatric LT recipients. The recipients were monitored for CMV infection and CMV-specific T cells from the start of immunosuppressive therapy until 48 weeks after LT. Clinically significant CMV viremia (csCMV) requiring preemptive therapy was defined as a CMV load of >2000 IU/mL. Peripheral blood CMV-specific T cells were analyzed by flow cytometry based on IFNγ secretion upon stimulation with CMV antigens including immediate early protein 1 (IE1) Ag, phosphoprotein 65 (pp65) Ag, and whole CMV lysate (wCMV). Of the 41 patients who underwent LT, 20 (48.8%) had csCMV. Most (17/20 patients) were asymptomatic and characterized as experiencing CMV reactivation. The onset of csCMV occurred approximately 7 weeks after LT (interquartile range: 4–12.9); csCMV rarely recurred after preemptive therapy. Lower pp65-specific CD8+ T cell response was associated with the occurrence of csCMV (<i>p</i> = 0.01) and correlated with increased viral load at the time of csCMV diagnosis (ρ = −0.553, <i>p</i> = 0.02). Moreover, those with csCMV had lower percentages of IE1-specific CD4+ and wCMV-reactive CD4+ T cells at 12 weeks after LT (<i>p</i> = 0.03 and <i>p</i> = 0.01, respectively). Despite intense immunosuppressive therapy, CMV-specific T cell immune reconstitution occurred in pediatric patients post-LT, which could confer protection against CMV reactivation. |
| first_indexed | 2024-03-09T16:23:08Z |
| format | Article |
| id | doaj.art-e6c29e94ef624dfca6d6fec2f4c57519 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T16:23:08Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e6c29e94ef624dfca6d6fec2f4c575192023-11-24T15:10:48ZengMDPI AGViruses1999-49152023-11-011511221310.3390/v15112213Cytomegalovirus-Specific T Cells in Pediatric Liver Transplant RecipientsSongpon Getsuwan0Nopporn Apiwattanakul1Chatmanee Lertudomphonwanit2Suradej Hongeng3Sophida Boonsathorn4Wiparat Manuyakorn5Pornthep Tanpowpong6Usanarat Anurathapan7Kanchana Tangnararatchakit8Suporn Treepongkaruna9Division of Gastroenterology, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Infectious Disease, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Gastroenterology, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Hematology and Oncology, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Infectious Disease, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Allergy, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Gastroenterology, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Hematology and Oncology, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Nephrology, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Gastroenterology, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandCytomegalovirus (CMV) infection is a major opportunistic infection after liver transplantation (LT) that necessitates monitoring. Because of the lack of studies in children, we aimed to investigate CMV-specific T cell immune reconstitution among pediatric LT recipients. The recipients were monitored for CMV infection and CMV-specific T cells from the start of immunosuppressive therapy until 48 weeks after LT. Clinically significant CMV viremia (csCMV) requiring preemptive therapy was defined as a CMV load of >2000 IU/mL. Peripheral blood CMV-specific T cells were analyzed by flow cytometry based on IFNγ secretion upon stimulation with CMV antigens including immediate early protein 1 (IE1) Ag, phosphoprotein 65 (pp65) Ag, and whole CMV lysate (wCMV). Of the 41 patients who underwent LT, 20 (48.8%) had csCMV. Most (17/20 patients) were asymptomatic and characterized as experiencing CMV reactivation. The onset of csCMV occurred approximately 7 weeks after LT (interquartile range: 4–12.9); csCMV rarely recurred after preemptive therapy. Lower pp65-specific CD8+ T cell response was associated with the occurrence of csCMV (<i>p</i> = 0.01) and correlated with increased viral load at the time of csCMV diagnosis (ρ = −0.553, <i>p</i> = 0.02). Moreover, those with csCMV had lower percentages of IE1-specific CD4+ and wCMV-reactive CD4+ T cells at 12 weeks after LT (<i>p</i> = 0.03 and <i>p</i> = 0.01, respectively). Despite intense immunosuppressive therapy, CMV-specific T cell immune reconstitution occurred in pediatric patients post-LT, which could confer protection against CMV reactivation.https://www.mdpi.com/1999-4915/15/11/2213cytomegalovirusliver transplantationchildrenimmunologyT cells |
| spellingShingle | Songpon Getsuwan Nopporn Apiwattanakul Chatmanee Lertudomphonwanit Suradej Hongeng Sophida Boonsathorn Wiparat Manuyakorn Pornthep Tanpowpong Usanarat Anurathapan Kanchana Tangnararatchakit Suporn Treepongkaruna Cytomegalovirus-Specific T Cells in Pediatric Liver Transplant Recipients Viruses cytomegalovirus liver transplantation children immunology T cells |
| title | Cytomegalovirus-Specific T Cells in Pediatric Liver Transplant Recipients |
| title_full | Cytomegalovirus-Specific T Cells in Pediatric Liver Transplant Recipients |
| title_fullStr | Cytomegalovirus-Specific T Cells in Pediatric Liver Transplant Recipients |
| title_full_unstemmed | Cytomegalovirus-Specific T Cells in Pediatric Liver Transplant Recipients |
| title_short | Cytomegalovirus-Specific T Cells in Pediatric Liver Transplant Recipients |
| title_sort | cytomegalovirus specific t cells in pediatric liver transplant recipients |
| topic | cytomegalovirus liver transplantation children immunology T cells |
| url | https://www.mdpi.com/1999-4915/15/11/2213 |
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