Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09
ABSTRACT A soil isolate, Penicillium janthinellum sw09 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as exo-polygalacturonase (exo-PG). By optimizing growth conditions, P. janthinellum sw09 produced high amount of exo-PG (16.54 units/mL). The...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2016-01-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200479&lng=en&tlng=en |
| _version_ | 1828976190529994752 |
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| author | YUPING MA SIWEN SUN HUI HAO CHUNPING XU |
| author_facet | YUPING MA SIWEN SUN HUI HAO CHUNPING XU |
| author_sort | YUPING MA |
| collection | DOAJ |
| description | ABSTRACT A soil isolate, Penicillium janthinellum sw09 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as exo-polygalacturonase (exo-PG). By optimizing growth conditions, P. janthinellum sw09 produced high amount of exo-PG (16.54 units/mL). The crude enzyme was purified by gel filtration chromatography and two exo-PG activity peaks (designated as PGI and PGII) were revealed. On SDS-PAGE analysis, purified PGII using DEAE-Sepharose FF column, was found to be a single band with a molecular mass of 66.2 kDa. The purified PGII exhibited maximal activity at the temperature of 45 oC and pH 5.0. The stability profiles show that PGII is more stable in the pH range of 4.0-8.0 and below 60 oC. The Km and Vmax for the enzyme was 1.74 mg/mL and 18.08 μmol/ (mL•min), respectively. Due to this enzymatic characterization, this pectinase is an attractive candidate for applications in degradation of pectin. |
| first_indexed | 2024-12-14T14:33:25Z |
| format | Article |
| id | doaj.art-e6c304bd8f324b658217f01be72694ea |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-14T14:33:25Z |
| publishDate | 2016-01-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-e6c304bd8f324b658217f01be72694ea2022-12-21T22:57:44ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902016-01-0188suppl 147948710.1590/0001-3765201620150051S0001-37652016000200479Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09YUPING MASIWEN SUNHUI HAOCHUNPING XUABSTRACT A soil isolate, Penicillium janthinellum sw09 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as exo-polygalacturonase (exo-PG). By optimizing growth conditions, P. janthinellum sw09 produced high amount of exo-PG (16.54 units/mL). The crude enzyme was purified by gel filtration chromatography and two exo-PG activity peaks (designated as PGI and PGII) were revealed. On SDS-PAGE analysis, purified PGII using DEAE-Sepharose FF column, was found to be a single band with a molecular mass of 66.2 kDa. The purified PGII exhibited maximal activity at the temperature of 45 oC and pH 5.0. The stability profiles show that PGII is more stable in the pH range of 4.0-8.0 and below 60 oC. The Km and Vmax for the enzyme was 1.74 mg/mL and 18.08 μmol/ (mL•min), respectively. Due to this enzymatic characterization, this pectinase is an attractive candidate for applications in degradation of pectin.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200479&lng=en&tlng=enPenicillium janthinellumpoligalacturonasepurificaçãocaracterização |
| spellingShingle | YUPING MA SIWEN SUN HUI HAO CHUNPING XU Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09 Anais da Academia Brasileira de Ciências Penicillium janthinellum poligalacturonase purificação caracterização |
| title | Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09 |
| title_full | Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09 |
| title_fullStr | Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09 |
| title_full_unstemmed | Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09 |
| title_short | Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09 |
| title_sort | production purification and characterization of an exo polygalacturonase from penicillium janthinellum sw09 |
| topic | Penicillium janthinellum poligalacturonase purificação caracterização |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200479&lng=en&tlng=en |
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