Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia
Human enterovirus 71 (EV-A71) causes hand, foot and mouth disease (HFMD). EV-A71 circulates in many countries and has caused large epidemics, especially in the Asia-Pacific region, since 1997. In April 2012, an undiagnosed fatal disease with neurological involvement and respiratory distress occurred...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1038/emi.2016.101 |
| _version_ | 1797679126148022272 |
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| author | Veasna Duong Channa Mey Marc Eloit Huachen Zhu Lucie Danet Zhong Huang Gang Zou Arnaud Tarantola Justine Cheval Philippe Perot Denis Laurent Beat Richner Santy Ky Sothy Heng Sok Touch Ly Sovann Rogier van Doorn Thanh Tan Tran Jeremy J Farrar David E Wentworth Suman R Das Timothy B Stockwell Jean-Claude Manuguerra Francis Delpeyroux Yi Guan Ralf Altmeyer Philippe Buchy |
| author_facet | Veasna Duong Channa Mey Marc Eloit Huachen Zhu Lucie Danet Zhong Huang Gang Zou Arnaud Tarantola Justine Cheval Philippe Perot Denis Laurent Beat Richner Santy Ky Sothy Heng Sok Touch Ly Sovann Rogier van Doorn Thanh Tan Tran Jeremy J Farrar David E Wentworth Suman R Das Timothy B Stockwell Jean-Claude Manuguerra Francis Delpeyroux Yi Guan Ralf Altmeyer Philippe Buchy |
| author_sort | Veasna Duong |
| collection | DOAJ |
| description | Human enterovirus 71 (EV-A71) causes hand, foot and mouth disease (HFMD). EV-A71 circulates in many countries and has caused large epidemics, especially in the Asia-Pacific region, since 1997. In April 2012, an undiagnosed fatal disease with neurological involvement and respiratory distress occurred in young children admitted to the Kantha Bopha Children’s Hospital in Phnom Penh, Cambodia. Most died within a day of hospital admission, causing public panic and international concern. In this study, we describe the enterovirus (EV) genotypes that were isolated during the outbreak in 2012 and the following year. From June 2012 to November 2013, 312 specimens were collected from hospitalized and ambulatory patients and tested by generic EV and specific EV-A71 reverse transcription PCR. EV-A71 was detected in 208 clinical specimens while other EVs were found in 32 patients. The VP1 gene and/or the complete genome were generated. Our phylogenetic sequencing analysis demonstrated that 80 EV-A71 strains belonged to the C4a subgenotype and 3 EV-A71 strains belonged to the B5 genotype. Furthermore, some lineages of EV-A71 were found to have appeared in Cambodia following separate introductions from neighboring countries. Nineteen EV A (CV-A6 and CV-A16), 9 EV B (EV-B83, CV-B3, CV-B2, CV-A9, E-31, E-2 and EV-B80) and 4 EV C (EV-C116, EV-C96, CV-A20 and Vaccine-related PV-3) strains were also detected. We found no molecular markers of disease severity. We report here that EV-A71 genotype C4 was the main etiological agent of a large outbreak of HFMD and particularly of severe forms associated with central nervous system infections. The role played by other EVs in the epidemic could not be clearly established. |
| first_indexed | 2024-03-11T23:09:52Z |
| format | Article |
| id | doaj.art-e6c5363df12c4990951c6ee105807955 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-11T23:09:52Z |
| publishDate | 2016-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-e6c5363df12c4990951c6ee1058079552023-09-21T12:27:55ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512016-01-01511910.1038/emi.2016.101Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in CambodiaVeasna Duong0Channa Mey1Marc Eloit2Huachen Zhu3Lucie Danet4Zhong Huang5Gang Zou6Arnaud Tarantola7Justine Cheval8Philippe Perot9Denis Laurent10Beat Richner11Santy Ky12Sothy Heng13Sok Touch14Ly Sovann15Rogier van Doorn16Thanh Tan Tran17Jeremy J Farrar18David E Wentworth19Suman R Das20Timothy B Stockwell21Jean-Claude Manuguerra22Francis Delpeyroux23Yi Guan24Ralf Altmeyer25Philippe Buchy26Pasteur Institute in Cambodia, Phnom Penh 12000, CambodiaPasteur Institute in Cambodia, Phnom Penh 12000, CambodiaPasteur Institute, Paris 75724, FranceState Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong, ChinaPasteur Institute in Cambodia, Phnom Penh 12000, CambodiaInstitut Pasteur in Shanghai, Shanghai 200025, ChinaInstitut Pasteur in Shanghai, Shanghai 200025, ChinaPasteur Institute in Cambodia, Phnom Penh 12000, CambodiaPathoQuest, Paris 75015, FrancePasteur Institute, Paris 75724, FranceKantha Bopha Hospital, Phnom Penh 12000, CambodiaKantha Bopha Hospital, Phnom Penh 12000, CambodiaKantha Bopha Hospital, Phnom Penh 12000, CambodiaKantha Bopha Hospital, Phnom Penh 12000, CambodiaMinistry of Health, Phnom Penh 12000, CambodiaMinistry of Health, Phnom Penh 12000, CambodiaOxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh P1Q5, VietnamOxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh P1Q5, VietnamOxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh P1Q5, VietnamJ. Craig Venter Institute, Rockville, MD 92037, USAJ. Craig Venter Institute, Rockville, MD 92037, USAJ. Craig Venter Institute, Rockville, MD 92037, USAPasteur Institute, Paris 75724, FrancePasteur Institute, Paris 75724, FranceState Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong, ChinaInstitut Pasteur in Shanghai, Shanghai 200025, ChinaPasteur Institute in Cambodia, Phnom Penh 12000, CambodiaHuman enterovirus 71 (EV-A71) causes hand, foot and mouth disease (HFMD). EV-A71 circulates in many countries and has caused large epidemics, especially in the Asia-Pacific region, since 1997. In April 2012, an undiagnosed fatal disease with neurological involvement and respiratory distress occurred in young children admitted to the Kantha Bopha Children’s Hospital in Phnom Penh, Cambodia. Most died within a day of hospital admission, causing public panic and international concern. In this study, we describe the enterovirus (EV) genotypes that were isolated during the outbreak in 2012 and the following year. From June 2012 to November 2013, 312 specimens were collected from hospitalized and ambulatory patients and tested by generic EV and specific EV-A71 reverse transcription PCR. EV-A71 was detected in 208 clinical specimens while other EVs were found in 32 patients. The VP1 gene and/or the complete genome were generated. Our phylogenetic sequencing analysis demonstrated that 80 EV-A71 strains belonged to the C4a subgenotype and 3 EV-A71 strains belonged to the B5 genotype. Furthermore, some lineages of EV-A71 were found to have appeared in Cambodia following separate introductions from neighboring countries. Nineteen EV A (CV-A6 and CV-A16), 9 EV B (EV-B83, CV-B3, CV-B2, CV-A9, E-31, E-2 and EV-B80) and 4 EV C (EV-C116, EV-C96, CV-A20 and Vaccine-related PV-3) strains were also detected. We found no molecular markers of disease severity. We report here that EV-A71 genotype C4 was the main etiological agent of a large outbreak of HFMD and particularly of severe forms associated with central nervous system infections. The role played by other EVs in the epidemic could not be clearly established.https://www.tandfonline.com/doi/10.1038/emi.2016.101Cambodiaepidemichand foot and mouth diseasehuman enterovirus 71severe disease |
| spellingShingle | Veasna Duong Channa Mey Marc Eloit Huachen Zhu Lucie Danet Zhong Huang Gang Zou Arnaud Tarantola Justine Cheval Philippe Perot Denis Laurent Beat Richner Santy Ky Sothy Heng Sok Touch Ly Sovann Rogier van Doorn Thanh Tan Tran Jeremy J Farrar David E Wentworth Suman R Das Timothy B Stockwell Jean-Claude Manuguerra Francis Delpeyroux Yi Guan Ralf Altmeyer Philippe Buchy Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia Emerging Microbes and Infections Cambodia epidemic hand foot and mouth disease human enterovirus 71 severe disease |
| title | Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia |
| title_full | Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia |
| title_fullStr | Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia |
| title_full_unstemmed | Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia |
| title_short | Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia |
| title_sort | molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand foot and mouth disease cases in cambodia |
| topic | Cambodia epidemic hand foot and mouth disease human enterovirus 71 severe disease |
| url | https://www.tandfonline.com/doi/10.1038/emi.2016.101 |
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