Targeting motifs in frustule-associated proteins from the centric diatom Thalassiosira pseudonana
The frustule of diatoms has an exceptional structure composed of inorganic and organic molecules. In the organic fraction, protein families were identified whose members are expected to have a complex cellular targeting to their final location within the frustule. Here we investigated for frustule-t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1006072/full |
| _version_ | 1797989664540327936 |
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| author | Neri Fattorini Uwe G. Maier Uwe G. Maier |
| author_facet | Neri Fattorini Uwe G. Maier Uwe G. Maier |
| author_sort | Neri Fattorini |
| collection | DOAJ |
| description | The frustule of diatoms has an exceptional structure composed of inorganic and organic molecules. In the organic fraction, protein families were identified whose members are expected to have a complex cellular targeting to their final location within the frustule. Here we investigated for frustule-targeting signals two representatives of the cingulin family, the proteins CinY2 and CinW2; beside an already known, classical signal peptide, we have identified further regions involved in cellular targeting. By using these regions as a search criteria we were able to identify two new frustule proteins. In addition, we showed that the temporal regulation of the gene expression determines the final location of one cingulin. Our results therefore point to a sophisticated cellular and extracellular targeting of frustule components to build the fascinating frustule structure of a diatom. |
| first_indexed | 2024-04-11T08:24:00Z |
| format | Article |
| id | doaj.art-e6ca527dc71f4895bb7be8b2fd29bb30 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T08:24:00Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e6ca527dc71f4895bb7be8b2fd29bb302022-12-22T04:34:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10060721006072Targeting motifs in frustule-associated proteins from the centric diatom Thalassiosira pseudonanaNeri Fattorini0Uwe G. Maier1Uwe G. Maier2Department of Biology, Laboratory for Cell Biology Philipps-University Marburg, Marburg, GermanyDepartment of Biology, Laboratory for Cell Biology Philipps-University Marburg, Marburg, GermanyCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, GermanyThe frustule of diatoms has an exceptional structure composed of inorganic and organic molecules. In the organic fraction, protein families were identified whose members are expected to have a complex cellular targeting to their final location within the frustule. Here we investigated for frustule-targeting signals two representatives of the cingulin family, the proteins CinY2 and CinW2; beside an already known, classical signal peptide, we have identified further regions involved in cellular targeting. By using these regions as a search criteria we were able to identify two new frustule proteins. In addition, we showed that the temporal regulation of the gene expression determines the final location of one cingulin. Our results therefore point to a sophisticated cellular and extracellular targeting of frustule components to build the fascinating frustule structure of a diatom.https://www.frontiersin.org/articles/10.3389/fpls.2022.1006072/fullsilaffinscingulinsfrustulesilicapentalysine clusterslysine-enriched regions |
| spellingShingle | Neri Fattorini Uwe G. Maier Uwe G. Maier Targeting motifs in frustule-associated proteins from the centric diatom Thalassiosira pseudonana Frontiers in Plant Science silaffins cingulins frustule silica pentalysine clusters lysine-enriched regions |
| title | Targeting motifs in frustule-associated proteins from the centric diatom Thalassiosira pseudonana |
| title_full | Targeting motifs in frustule-associated proteins from the centric diatom Thalassiosira pseudonana |
| title_fullStr | Targeting motifs in frustule-associated proteins from the centric diatom Thalassiosira pseudonana |
| title_full_unstemmed | Targeting motifs in frustule-associated proteins from the centric diatom Thalassiosira pseudonana |
| title_short | Targeting motifs in frustule-associated proteins from the centric diatom Thalassiosira pseudonana |
| title_sort | targeting motifs in frustule associated proteins from the centric diatom thalassiosira pseudonana |
| topic | silaffins cingulins frustule silica pentalysine clusters lysine-enriched regions |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1006072/full |
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