Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper
In this paper, we present a novel and cost-effective lab-on-paper microfluidics platform for performing ELISA autonomously, with no user intervention beyond adding the sample. The platform utilizes two Bi-Material Cantilever Valves placed in a specially designed housing. The integration of these val...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/13/3/310 |
| _version_ | 1797613236942536704 |
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| author | Hojat Heidari-Bafroui Ashutosh Kumar Cameron Hahn Nicholas Scholz Amer Charbaji Nassim Rahmani Constantine Anagnostopoulos Mohammad Faghri |
| author_facet | Hojat Heidari-Bafroui Ashutosh Kumar Cameron Hahn Nicholas Scholz Amer Charbaji Nassim Rahmani Constantine Anagnostopoulos Mohammad Faghri |
| author_sort | Hojat Heidari-Bafroui |
| collection | DOAJ |
| description | In this paper, we present a novel and cost-effective lab-on-paper microfluidics platform for performing ELISA autonomously, with no user intervention beyond adding the sample. The platform utilizes two Bi-Material Cantilever Valves placed in a specially designed housing. The integration of these valves in a specific channel network forms a complete fluidic logic circuit for performing ELISA on paper. The housing also incorporates an innovative reagent storage and release mechanism that minimizes variability in the volume of reagents released into the reagent pads. The platform design was optimized to minimize variance in the time of fluid wicking from the reagent pad, using a randomized design of experiment. The platform adheres to the World Health Organization’s ASSURED principles. The optimized design was used to conduct an ELISA for detecting rabbit immunoglobulin G (IgG) in a buffer, with a limit of detection of 2.27 ng/mL and a limit of quantification of 8.33 ng/mL. This represents a 58% improvement over previous ELISA methods for detecting rabbit IgG in buffer using portable microfluidic technology. |
| first_indexed | 2024-03-11T06:52:03Z |
| format | Article |
| id | doaj.art-e6cb670be33a480087254900a15200f9 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T06:52:03Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-e6cb670be33a480087254900a15200f92023-11-17T09:53:34ZengMDPI AGBiosensors2079-63742023-02-0113331010.3390/bios13030310Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on PaperHojat Heidari-Bafroui0Ashutosh Kumar1Cameron Hahn2Nicholas Scholz3Amer Charbaji4Nassim Rahmani5Constantine Anagnostopoulos6Mohammad Faghri7Microfluidics Laboratory, Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, 2 East Alumni Avenue, Kingston, RI 02881, USAMicrofluidics Laboratory, Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, 2 East Alumni Avenue, Kingston, RI 02881, USAMicrofluidics Laboratory, Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, 2 East Alumni Avenue, Kingston, RI 02881, USAMicrofluidics Laboratory, Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, 2 East Alumni Avenue, Kingston, RI 02881, USAMicrofluidics Laboratory, Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, 2 East Alumni Avenue, Kingston, RI 02881, USAMicrofluidics Laboratory, Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, 2 East Alumni Avenue, Kingston, RI 02881, USAMicrofluidics Laboratory, Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, 2 East Alumni Avenue, Kingston, RI 02881, USAMicrofluidics Laboratory, Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, 2 East Alumni Avenue, Kingston, RI 02881, USAIn this paper, we present a novel and cost-effective lab-on-paper microfluidics platform for performing ELISA autonomously, with no user intervention beyond adding the sample. The platform utilizes two Bi-Material Cantilever Valves placed in a specially designed housing. The integration of these valves in a specific channel network forms a complete fluidic logic circuit for performing ELISA on paper. The housing also incorporates an innovative reagent storage and release mechanism that minimizes variability in the volume of reagents released into the reagent pads. The platform design was optimized to minimize variance in the time of fluid wicking from the reagent pad, using a randomized design of experiment. The platform adheres to the World Health Organization’s ASSURED principles. The optimized design was used to conduct an ELISA for detecting rabbit immunoglobulin G (IgG) in a buffer, with a limit of detection of 2.27 ng/mL and a limit of quantification of 8.33 ng/mL. This represents a 58% improvement over previous ELISA methods for detecting rabbit IgG in buffer using portable microfluidic technology.https://www.mdpi.com/2079-6374/13/3/310colorimetric assaypaper-based deviceslab-on-paperELISApoint-of-care diagnosticssensors |
| spellingShingle | Hojat Heidari-Bafroui Ashutosh Kumar Cameron Hahn Nicholas Scholz Amer Charbaji Nassim Rahmani Constantine Anagnostopoulos Mohammad Faghri Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper Biosensors colorimetric assay paper-based devices lab-on-paper ELISA point-of-care diagnostics sensors |
| title | Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper |
| title_full | Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper |
| title_fullStr | Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper |
| title_full_unstemmed | Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper |
| title_short | Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper |
| title_sort | development of a new lab on paper microfluidics platform using bi material cantilever actuators for elisa on paper |
| topic | colorimetric assay paper-based devices lab-on-paper ELISA point-of-care diagnostics sensors |
| url | https://www.mdpi.com/2079-6374/13/3/310 |
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