Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum
Some well-known Clostridiales species such as Clostridium difficile and C. perfringens are agents of high impact diseases worldwide. Nevertheless, other foreseen Clostridiales species have recently emerged such as Clostridium tertium and C. paraputrificum. Three fecal isolates were identified as Clo...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
|
| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2019.1637699 |
| _version_ | 1828855049214754816 |
|---|---|
| author | Marina Muñoz Daniel Restrepo-Montoya Nitin Kumar Gregorio Iraola Giovanny Herrera Dora I. Ríos-Chaparro Diana Díaz-Arévalo Manuel A. Patarroyo Trevor D. Lawley Juan David Ramírez |
| author_facet | Marina Muñoz Daniel Restrepo-Montoya Nitin Kumar Gregorio Iraola Giovanny Herrera Dora I. Ríos-Chaparro Diana Díaz-Arévalo Manuel A. Patarroyo Trevor D. Lawley Juan David Ramírez |
| author_sort | Marina Muñoz |
| collection | DOAJ |
| description | Some well-known Clostridiales species such as Clostridium difficile and C. perfringens are agents of high impact diseases worldwide. Nevertheless, other foreseen Clostridiales species have recently emerged such as Clostridium tertium and C. paraputrificum. Three fecal isolates were identified as Clostridium tertium (Gcol.A2 and Gcol.A43) and C. paraputrificum (Gcol.A11) during public health screening for C. difficile infections in Colombia. C. paraputrificum genomes were highly diverse and contained large numbers of accessory genes. Genetic diversity and accessory gene percentage were lower among the C. tertium genomes than in the C. paraputrificum genomes. C. difficile tcdA and tcdB toxins encoding homologous sequences and other potential virulence factors were also identified. EndoA interferase, a toxic component of the type II toxin-antitoxin system, was found among the C. tertium genomes. toxA was the only toxin encoding gene detected in Gcol.A43, the Colombian isolate with an experimentally-determined high cytotoxic effect. Gcol.A2 and Gcol.A43 had higher sporulation efficiencies than Gcol.A11 (84.5%, 83.8% and 57.0%, respectively), as supported by the greater number of proteins associated with sporulation pathways in the C. tertium genomes compared with the C. paraputrificum genomes (33.3 and 28.4 on average, respectively). This work allowed complete genome description of two clostridiales species revealing high levels of intra-taxa diversity, accessory genomes containing virulence-factors encoding genes (especially in C. paraputrificum), with proteins involved in sporulation processes more highly represented in C. tertium. These finding suggest the need to advance in the study of those species with potential importance at public health level. |
| first_indexed | 2024-12-13T00:47:18Z |
| format | Article |
| id | doaj.art-e6ce66bbde284ca29ab45590f44f6a2e |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-12-13T00:47:18Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-e6ce66bbde284ca29ab45590f44f6a2e2022-12-22T00:05:01ZengTaylor & Francis GroupVirulence2150-55942150-56082019-01-0110165767610.1080/21505594.2019.16376991637699Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificumMarina Muñoz0Daniel Restrepo-Montoya1Nitin Kumar2Gregorio Iraola3Giovanny Herrera4Dora I. Ríos-Chaparro5Diana Díaz-Arévalo6Manuel A. Patarroyo7Trevor D. Lawley8Juan David Ramírez9Universidad del RosarioUniversidad del RosarioWellcome Trust Sanger InstituteInstitut Pasteur MontevideoUniversidad del RosarioUniversidad del RosarioFundación Instituto de Inmunología de Colombia (FIDIC)Fundación Instituto de Inmunología de Colombia (FIDIC)Wellcome Trust Sanger InstituteUniversidad del RosarioSome well-known Clostridiales species such as Clostridium difficile and C. perfringens are agents of high impact diseases worldwide. Nevertheless, other foreseen Clostridiales species have recently emerged such as Clostridium tertium and C. paraputrificum. Three fecal isolates were identified as Clostridium tertium (Gcol.A2 and Gcol.A43) and C. paraputrificum (Gcol.A11) during public health screening for C. difficile infections in Colombia. C. paraputrificum genomes were highly diverse and contained large numbers of accessory genes. Genetic diversity and accessory gene percentage were lower among the C. tertium genomes than in the C. paraputrificum genomes. C. difficile tcdA and tcdB toxins encoding homologous sequences and other potential virulence factors were also identified. EndoA interferase, a toxic component of the type II toxin-antitoxin system, was found among the C. tertium genomes. toxA was the only toxin encoding gene detected in Gcol.A43, the Colombian isolate with an experimentally-determined high cytotoxic effect. Gcol.A2 and Gcol.A43 had higher sporulation efficiencies than Gcol.A11 (84.5%, 83.8% and 57.0%, respectively), as supported by the greater number of proteins associated with sporulation pathways in the C. tertium genomes compared with the C. paraputrificum genomes (33.3 and 28.4 on average, respectively). This work allowed complete genome description of two clostridiales species revealing high levels of intra-taxa diversity, accessory genomes containing virulence-factors encoding genes (especially in C. paraputrificum), with proteins involved in sporulation processes more highly represented in C. tertium. These finding suggest the need to advance in the study of those species with potential importance at public health level.http://dx.doi.org/10.1080/21505594.2019.1637699clostridium tertiumclostridium paraputrificumclostridial speciesgenetic diversityvirulence factors |
| spellingShingle | Marina Muñoz Daniel Restrepo-Montoya Nitin Kumar Gregorio Iraola Giovanny Herrera Dora I. Ríos-Chaparro Diana Díaz-Arévalo Manuel A. Patarroyo Trevor D. Lawley Juan David Ramírez Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum Virulence clostridium tertium clostridium paraputrificum clostridial species genetic diversity virulence factors |
| title | Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum |
| title_full | Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum |
| title_fullStr | Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum |
| title_full_unstemmed | Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum |
| title_short | Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum |
| title_sort | comparative genomics identifies potential virulence factors in clostridium tertium and c paraputrificum |
| topic | clostridium tertium clostridium paraputrificum clostridial species genetic diversity virulence factors |
| url | http://dx.doi.org/10.1080/21505594.2019.1637699 |
| work_keys_str_mv | AT marinamunoz comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum AT danielrestrepomontoya comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum AT nitinkumar comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum AT gregorioiraola comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum AT giovannyherrera comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum AT dorairioschaparro comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum AT dianadiazarevalo comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum AT manuelapatarroyo comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum AT trevordlawley comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum AT juandavidramirez comparativegenomicsidentifiespotentialvirulencefactorsinclostridiumtertiumandcparaputrificum |