Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues
The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source,...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2009-11-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022 |
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| author | Luiz Romulo Alberton Luciana Porto de Souza Vandenberghe Ricardo Assmann Ricardo Cancio Fendrich José Rodriguéz-León Carlos Ricardo Soccol |
| author_facet | Luiz Romulo Alberton Luciana Porto de Souza Vandenberghe Ricardo Assmann Ricardo Cancio Fendrich José Rodriguéz-León Carlos Ricardo Soccol |
| author_sort | Luiz Romulo Alberton |
| collection | DOAJ |
| description | The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the highest xylanase activity (423.9 U/g) was reached with: 70 % sugarcane bagasse, 20% Napier grass and 10% soybean meal, 90% of moisture, and 10(7)/g substrate.<br>A produção de xilanase por Streptomyces viridosporus T7A foi realizada em fermentação submersa (FSm) e fermentação no estado sólido (FES) utilizando resíduos e sub-produtos agroindustriais. Capim Napier, bagaço de cana e farelo de soja foram empregados como fonte de carbono, suporte/substrato e fonte nitrogênio, respectivamente. Em FSm, o capim Napier (1 % p/v) foi suplementado com farelo de soja (1,5 % p/v), hidroxietilcelulose (0,15 %) e vitaminas do complexo B (1,5 %) sendo que a produção de xilanase atingiu 23.41 U/mL. Em FES, o efeito dos seguintes parâmetros foi estudado: composição do substrato (bagaço de cana, Capim Napier e farelo de soja), umidade inicial, aeração e taxa de inoculação. A mais elevada produção de xilanase (423,9 U/g) foi atingida com 70% bagaço de cana, 20% de capim Napier e 10 % farelo de soja, 90 % de umidade inicial e 10(7) células/g substrato. |
| first_indexed | 2024-12-18T23:24:56Z |
| format | Article |
| id | doaj.art-e6ced81165764afcb8c3dd922da03d52 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-18T23:24:56Z |
| publishDate | 2009-11-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-e6ced81165764afcb8c3dd922da03d522022-12-21T20:47:50ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242009-11-0152spe17118010.1590/S1516-89132009000700022Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residuesLuiz Romulo AlbertonLuciana Porto de Souza VandenbergheRicardo AssmannRicardo Cancio FendrichJosé Rodriguéz-LeónCarlos Ricardo SoccolThe study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the highest xylanase activity (423.9 U/g) was reached with: 70 % sugarcane bagasse, 20% Napier grass and 10% soybean meal, 90% of moisture, and 10(7)/g substrate.<br>A produção de xilanase por Streptomyces viridosporus T7A foi realizada em fermentação submersa (FSm) e fermentação no estado sólido (FES) utilizando resíduos e sub-produtos agroindustriais. Capim Napier, bagaço de cana e farelo de soja foram empregados como fonte de carbono, suporte/substrato e fonte nitrogênio, respectivamente. Em FSm, o capim Napier (1 % p/v) foi suplementado com farelo de soja (1,5 % p/v), hidroxietilcelulose (0,15 %) e vitaminas do complexo B (1,5 %) sendo que a produção de xilanase atingiu 23.41 U/mL. Em FES, o efeito dos seguintes parâmetros foi estudado: composição do substrato (bagaço de cana, Capim Napier e farelo de soja), umidade inicial, aeração e taxa de inoculação. A mais elevada produção de xilanase (423,9 U/g) foi atingida com 70% bagaço de cana, 20% de capim Napier e 10 % farelo de soja, 90 % de umidade inicial e 10(7) células/g substrato.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022Streptomycesxylanasesubmerged fermentationsolid-state agro-industrial fermentationresidues |
| spellingShingle | Luiz Romulo Alberton Luciana Porto de Souza Vandenberghe Ricardo Assmann Ricardo Cancio Fendrich José Rodriguéz-León Carlos Ricardo Soccol Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues Brazilian Archives of Biology and Technology Streptomyces xylanase submerged fermentation solid-state agro-industrial fermentation residues |
| title | Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues |
| title_full | Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues |
| title_fullStr | Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues |
| title_full_unstemmed | Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues |
| title_short | Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues |
| title_sort | xylanase production by streptomyces viridosporus t7a in submerged and solid state fermentation using agro industrial residues |
| topic | Streptomyces xylanase submerged fermentation solid-state agro-industrial fermentation residues |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022 |
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