Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis
Previous studies have suggested that the Lactobacillus plantarum bacteria strain could be effective in ulcerative colitis (UC) management. However, its effects are strain-specific and the related mechanisms for its attenuating effects on UC remain unclear. This study aimed to elucidate the underlyin...
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.575173/full |
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| author | Peng Yu Chuxin Ke Jiaxin Guo Xiuling Zhang Bailiang Li Bailiang Li |
| author_facet | Peng Yu Chuxin Ke Jiaxin Guo Xiuling Zhang Bailiang Li Bailiang Li |
| author_sort | Peng Yu |
| collection | DOAJ |
| description | Previous studies have suggested that the Lactobacillus plantarum bacteria strain could be effective in ulcerative colitis (UC) management. However, its effects are strain-specific and the related mechanisms for its attenuating effects on UC remain unclear. This study aimed to elucidate the underlying mechanisms for the protective effect of L. plantarum on UC. Firstly, 15 L. plantarum strains were screened for potential probiotic characteristics with good tolerance to simulated human gastrointestinal transit and adhesion. Secondly, the inflammatory response of selected strains to the Caco-2 cells induced by lipopolysaccharide (LPS) was measured. Finally, an in vivo mouse model induced by dextran sulfate sodium (DSS) was used to assess the beneficial effects and likely action mechanisms the successfully screened in vitro strain, L. plantarum L15. In vitro results showed that L. plantarum L15 possessed the highest gastrointestinal transit tolerance, adhesion and reduction of pro-inflammatory abilities compared to the other screened strains. In vivo, high dose of L. plantarum L15 supplementation increased the body weight, colon length and anti-inflammatory cytokine production. Pro-inflammatory cytokine production, disease activity index (DAI) levels and myeloperoxidase (MPO) parameters decreased using this strain. In addition, L. plantarum L15 alleviated the histopathological changes in colon, modulated the gut microbiota, and decreased LPS secretion. The activities of this strain down-regulated the expression of TLR4 and MyD88 genes as well as genes associated with NF-κB signaling pathway. Our findings present L. plantarum L15 as a new probiotic, with promising application for UC management. |
| first_indexed | 2024-12-14T22:06:05Z |
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| language | English |
| last_indexed | 2024-12-14T22:06:05Z |
| publishDate | 2020-10-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-e6cee7802d4f49ad9219be789a4c56862022-12-21T22:45:52ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-10-011110.3389/fimmu.2020.575173575173Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota DysbiosisPeng Yu0Chuxin Ke1Jiaxin Guo2Xiuling Zhang3Bailiang Li4Bailiang Li5College of Food Science, Northeast Agricultural University, Harbin, ChinaCollege of Food Science, Northeast Agricultural University, Harbin, ChinaCollege of Food Science, Northeast Agricultural University, Harbin, ChinaCollege of Food Science, Northeast Agricultural University, Harbin, ChinaCollege of Food Science, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, ChinaPrevious studies have suggested that the Lactobacillus plantarum bacteria strain could be effective in ulcerative colitis (UC) management. However, its effects are strain-specific and the related mechanisms for its attenuating effects on UC remain unclear. This study aimed to elucidate the underlying mechanisms for the protective effect of L. plantarum on UC. Firstly, 15 L. plantarum strains were screened for potential probiotic characteristics with good tolerance to simulated human gastrointestinal transit and adhesion. Secondly, the inflammatory response of selected strains to the Caco-2 cells induced by lipopolysaccharide (LPS) was measured. Finally, an in vivo mouse model induced by dextran sulfate sodium (DSS) was used to assess the beneficial effects and likely action mechanisms the successfully screened in vitro strain, L. plantarum L15. In vitro results showed that L. plantarum L15 possessed the highest gastrointestinal transit tolerance, adhesion and reduction of pro-inflammatory abilities compared to the other screened strains. In vivo, high dose of L. plantarum L15 supplementation increased the body weight, colon length and anti-inflammatory cytokine production. Pro-inflammatory cytokine production, disease activity index (DAI) levels and myeloperoxidase (MPO) parameters decreased using this strain. In addition, L. plantarum L15 alleviated the histopathological changes in colon, modulated the gut microbiota, and decreased LPS secretion. The activities of this strain down-regulated the expression of TLR4 and MyD88 genes as well as genes associated with NF-κB signaling pathway. Our findings present L. plantarum L15 as a new probiotic, with promising application for UC management.https://www.frontiersin.org/article/10.3389/fimmu.2020.575173/fullLactobacillus plantarumDSS-colitisgut microbiotalipopolysaccharide (LPS)NF-κB signaling |
| spellingShingle | Peng Yu Chuxin Ke Jiaxin Guo Xiuling Zhang Bailiang Li Bailiang Li Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis Frontiers in Immunology Lactobacillus plantarum DSS-colitis gut microbiota lipopolysaccharide (LPS) NF-κB signaling |
| title | Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis |
| title_full | Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis |
| title_fullStr | Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis |
| title_full_unstemmed | Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis |
| title_short | Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis |
| title_sort | lactobacillus plantarum l15 alleviates colitis by inhibiting lps mediated nf κb activation and ameliorates dss induced gut microbiota dysbiosis |
| topic | Lactobacillus plantarum DSS-colitis gut microbiota lipopolysaccharide (LPS) NF-κB signaling |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2020.575173/full |
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