Stress Response of <i>Mesosutterella multiformis</i> Mediated by Nitrate Reduction
Bacterial stress responses are closely associated with the survival and colonization of anaerobes in the human gut. <i>Mesosutterella multiformis</i> JCM 32464<sup>T</sup> is a novel member of the family <i>Sutterellaceae</i>, an asaccharolytic bacterium. We previ...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/12/2003 |
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| author | Nao Ikeyama Moriya Ohkuma Mitsuo Sakamoto |
| author_facet | Nao Ikeyama Moriya Ohkuma Mitsuo Sakamoto |
| author_sort | Nao Ikeyama |
| collection | DOAJ |
| description | Bacterial stress responses are closely associated with the survival and colonization of anaerobes in the human gut. <i>Mesosutterella multiformis</i> JCM 32464<sup>T</sup> is a novel member of the family <i>Sutterellaceae</i>, an asaccharolytic bacterium. We previously demonstrated energy generation via heme biosynthesis, which is coupled with nitrate reductase. Here, physiological and morphological changes in <i>M. multiformis</i> induced by exposure to nitrate were investigated. The ability of <i>M. multiformis</i> to reduce nitrate was determined using a colorimetric assay. A unique morphology was observed during nitrate reduction under anaerobic conditions. The association between nitrate concentration and cell size or cellular fatty acid composition was evaluated. Nitrate-induced responses of <i>M. multiformis</i> were compared to those of related species. An increase in cellular filamentation and the ratio of saturated: unsaturated fatty acids was mediated specifically by nitrate. This indicates a decrease in cell fluidity and low leakage. Furthermore, a similar response was not observed in other related species cultured in the presence of nitrate. Hence, the nitrate-induced stress response in new anaerobes such as <i>M. multiformis</i> was demonstrated. The response could also be involved in the conservation of menaquinones and the maximization of nitrate reduction. |
| first_indexed | 2024-03-10T14:03:29Z |
| format | Article |
| id | doaj.art-e6d1fffbf2424a6aab02d9e9af875cce |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:03:29Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-e6d1fffbf2424a6aab02d9e9af875cce2023-11-21T00:54:49ZengMDPI AGMicroorganisms2076-26072020-12-01812200310.3390/microorganisms8122003Stress Response of <i>Mesosutterella multiformis</i> Mediated by Nitrate ReductionNao Ikeyama0Moriya Ohkuma1Mitsuo Sakamoto2Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba 305-0074, Ibaraki, JapanMicrobe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba 305-0074, Ibaraki, JapanMicrobe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba 305-0074, Ibaraki, JapanBacterial stress responses are closely associated with the survival and colonization of anaerobes in the human gut. <i>Mesosutterella multiformis</i> JCM 32464<sup>T</sup> is a novel member of the family <i>Sutterellaceae</i>, an asaccharolytic bacterium. We previously demonstrated energy generation via heme biosynthesis, which is coupled with nitrate reductase. Here, physiological and morphological changes in <i>M. multiformis</i> induced by exposure to nitrate were investigated. The ability of <i>M. multiformis</i> to reduce nitrate was determined using a colorimetric assay. A unique morphology was observed during nitrate reduction under anaerobic conditions. The association between nitrate concentration and cell size or cellular fatty acid composition was evaluated. Nitrate-induced responses of <i>M. multiformis</i> were compared to those of related species. An increase in cellular filamentation and the ratio of saturated: unsaturated fatty acids was mediated specifically by nitrate. This indicates a decrease in cell fluidity and low leakage. Furthermore, a similar response was not observed in other related species cultured in the presence of nitrate. Hence, the nitrate-induced stress response in new anaerobes such as <i>M. multiformis</i> was demonstrated. The response could also be involved in the conservation of menaquinones and the maximization of nitrate reduction.https://www.mdpi.com/2076-2607/8/12/2003<i>Mesosutterella multiformis</i>the family <i>Sutterellaceae</i>nitrate reductionnitratenitritefilament formation |
| spellingShingle | Nao Ikeyama Moriya Ohkuma Mitsuo Sakamoto Stress Response of <i>Mesosutterella multiformis</i> Mediated by Nitrate Reduction Microorganisms <i>Mesosutterella multiformis</i> the family <i>Sutterellaceae</i> nitrate reduction nitrate nitrite filament formation |
| title | Stress Response of <i>Mesosutterella multiformis</i> Mediated by Nitrate Reduction |
| title_full | Stress Response of <i>Mesosutterella multiformis</i> Mediated by Nitrate Reduction |
| title_fullStr | Stress Response of <i>Mesosutterella multiformis</i> Mediated by Nitrate Reduction |
| title_full_unstemmed | Stress Response of <i>Mesosutterella multiformis</i> Mediated by Nitrate Reduction |
| title_short | Stress Response of <i>Mesosutterella multiformis</i> Mediated by Nitrate Reduction |
| title_sort | stress response of i mesosutterella multiformis i mediated by nitrate reduction |
| topic | <i>Mesosutterella multiformis</i> the family <i>Sutterellaceae</i> nitrate reduction nitrate nitrite filament formation |
| url | https://www.mdpi.com/2076-2607/8/12/2003 |
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